##gff-version 3
##sequence-region BBa_K2066109 1 1922
BBa_K2066109	.	ribosome_entry_site	1041	1054	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_K2066109	.	conserved_region	1047	1050	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K2066109	.	terminator	1754	1882	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2066109	.	terminator	1842	1882	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2066109	.	engineered_region	1842	1882	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2066109	.	stem_loop	1849	1868	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2066109	.	polyA_site	1869	1882	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2066109	.	stop_codon	1875	1875	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2066109	.	terminator	1754	1833	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2066109	.	engineered_region	1754	1833	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2066109	.	stem_loop	1765	1808	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2066109	.	sequence_feature	978	997	.	+	0	ID=BBa_K2066507;Name=BBa_K2066507
BBa_K2066109	.	sequence_feature	1033	1040	.	+	0	ID=BBa_K2066529;Name=BBa_K2066529
BBa_K2066109	.	terminator	849	977	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2066109	.	terminator	937	977	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2066109	.	engineered_region	937	977	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2066109	.	stem_loop	944	963	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2066109	.	polyA_site	964	977	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2066109	.	stop_codon	970	970	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2066109	.	terminator	849	928	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2066109	.	engineered_region	849	928	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2066109	.	stem_loop	860	903	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2066109	.	sequence_feature	1	40	.	+	0	ID=BBa_K2066018;Name=BBa_K2066018
BBa_K2066109	.	promoter	41	94	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K2066109	.	promoter	83	88	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K2066109	.	promoter	60	65	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K2066109	.	engineered_region	41	94	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K2066109	.	non_covalent_binding_site	66	84	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K2066109	.	non_covalent_binding_site	41	59	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K2066109	.	sequence_feature	115	120	.	+	0	ID=BBa_K2066530;Name=BBa_K2066530
BBa_K2066109	.	sequence_feature	95	102	.	+	0	ID=BBa_K2066529;Name=BBa_K2066529
BBa_K2066109	.	sequence_feature	1746	1753	.	+	0	ID=BBa_K2066529;Name=BBa_K2066529
BBa_K2066109	.	sequence_feature	1055	1060	.	+	0	ID=BBa_K2066530;Name=BBa_K2066530
BBa_K2066109	.	CDS	121	840	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K2066109	.	CDS	121	840	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K2066109	.	promoter	998	1032	.	+	0	ID=BBa_J23105;Name=BBa_J23105
BBa_K2066109	.	ribosome_entry_site	103	114	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K2066109	.	conserved_region	107	110	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2066109	.	CDS	1061	1745	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_K2066109	.	CDS	1064	1680	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K2066109	.	engineered_tag	1721	1745	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K2066109	.	tag	1681	1714	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K2066109	.	sequence_feature	1883	1922	.	+	0	ID=BBa_K2066019;Name=BBa_K2066019
BBa_K2066109	.	sequence_feature	841	848	.	+	0	ID=BBa_K2066529;Name=BBa_K2066529
>BBa_K2066109
gctgggagttcgtagacggaaacaaacgcagaatccaagctccctatcagtgatagagattgacatccctatcagtgat
agagatactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtccc
aattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatac
ggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggtt
atggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggtta
tgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacc
cttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaact
ataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacat
tgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagac
aaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttg
taacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatactcgttcgctgccacctaagtttacggctagctcagtcctaggtactatg
ctagctactagagtcacacaggaaacctactagatgtccagattagataaaagtaaagtgattaacagcgcattagagc
tgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgta
ttggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgc
cctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcg
atggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatg
ccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaa
gatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagcta
tcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaaca
acttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgt
agatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagcactgaaggtcct
caatcgcactggaaacatcaaggtcg