LOCUS       BBa_K2066110            2388 bp    DNA     linear       17-May-2024
DEFINITION  pLacO1 sfGFP + LacI
ACCESSION   BBa_K2066110
VERSION     BBa_K2066110.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     promoter        41..118
                     /label=BBa_K2066508
     misc_feature    119..199
                     /label=BBa_K2066506
     RBS             200..211
                     /label=BBa_K2066510
     misc_feature    212..931
                     /label=BBa_K2066509
     stem_loop       943..986
                     /label=stem_loop
     misc_feature    932..1011
                     /label=BBa_B0010
     terminator      932..1011
                     /label=BBa_B0010
     stem_loop       1027..1046
                     /label=T7 TE
     misc_feature    1053..1053
                     /label=stop
     polyA_site      1047..1060
                     /label=polya
     misc_feature    1020..1060
                     /label=BBa_B0012
     terminator      1020..1060
                     /label=BBa_B0012
     terminator      932..1060
                     /label=BBa_B0015
     misc_feature    1061..1080
                     /label=BBa_K2066507
     promoter        1081..1115
                     /label=BBa_J23101
     misc_feature    1120..1123
                     /label=conserved
     RBS             1116..1127
                     /label=BBa_B0034
     CDS             1128..2219
                     /label=BBa_K2066524
     stem_loop       2231..2274
                     /label=stem_loop
     misc_feature    2220..2299
                     /label=BBa_B0010
     terminator      2220..2299
                     /label=BBa_B0010
     stem_loop       2315..2334
                     /label=T7 TE
     misc_feature    2341..2341
                     /label=stop
     polyA_site      2335..2348
                     /label=polya
     misc_feature    2308..2348
                     /label=BBa_B0012
     terminator      2308..2348
                     /label=BBa_B0012
     terminator      2220..2348
                     /label=BBa_B0015
     misc_feature    2349..2388
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc ggcaaatatt ctgaaatgag
       61 ctgataaatg tgagcggata acattgacat tgtgagcgga taacaagata ctgagcacag
      121 ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag cctctacaaa
      181 taattttgtt taaactagaa ggaggaaaaa aatgcgtaaa ggcgaagagc tgttcactgg
      241 tgtcgtccct attctggtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg
      301 tggcgagggt gaaggtgacg caactaatgg taaactgacg ctgaagttca tctgtactac
      361 tggtaaactg ccggtacctt ggccgactct ggtaacgacg ctgacttatg gtgttcagtg
      421 ctttgctcgt tatccggacc atatgaagca gcatgacttc ttcaagtccg ccatgccgga
      481 aggctatgtg caggaacgca cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc
      541 ggaagtgaaa tttgaaggcg ataccctggt aaaccgcatt gagctgaaag gcattgactt
      601 taaagaagac ggcaatatcc tgggccataa gctggaatac aattttaaca gccacaatgt
      661 gtacattacc gcagataaac aaaaaaatgg cattaaagcg aatttcaaaa ttcgccacaa
      721 cgtggaggat ggcagcgtgc agctggctga tcactaccag caaaacactc caatcggtga
      781 tggtcctgtt ctgctgccag acaatcacta tctgagcacg caaagcgttc tgtctaaaga
      841 tccgaacgag aaacgcgatc atatggttct gctggagttc gtaaccgcag cgggcatcac
      901 gcatggtatg gatgaactgt acaaatgatg accaggcatc aaataaaacg aaaggctcag
      961 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1021 cacactggct caccttcggg tgggcctttc tgcgtttata ctcgttcgct gccacctaag
     1081 tttacagcta gctcagtcct aggtattatg ctagcaaaga ggagaaaatg gtgaatgtga
     1141 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc
     1201 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga
     1261 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
     1321 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
     1381 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
     1441 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
     1501 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
     1561 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
     1621 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
     1681 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
     1741 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
     1801 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga
     1861 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
     1921 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
     1981 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
     2041 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
     2101 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt
     2161 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgac
     2221 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2281 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2341 cgtttatagc actgaaggtc ctcaatcgca ctggaaacat caaggtcg
//