BBa_K2066110
type: ComponentDefinition
persistent identity: BBa K2066110
display id: BBa_K2066110
version: 1
was generated by: iGEM to SBOL conversion
description: pLacO1 sfGFP + LacI
created: 2016-10-13T11:00:00Z
modified: 2016-10-28T09:01:44Z
mutable provenance: To be completed
owned by: james
owned by: myers
mutable description: To be completed
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 31547
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: To be completed
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K2066507
component: BBa_B0015
component: BBa_B0034
component: BBa_J23101
component: BBa_K2066524
component: BBa_K2066019
component: BBa_K2066018
component: BBa_K2066506
component: BBa_K2066508
component: BBa_K2066509
component: BBa_K2066510
sequence annotation: BBa_K2066018
sequence annotation: BBa_K2066508
sequence annotation: BBa_K2066506
sequence annotation: BBa_K2066510
sequence annotation: BBa_K2066509
sequence annotation: BBa_B0015
sequence annotation: BBa_K2066507
sequence annotation: BBa_J23101
sequence annotation: BBa_B0034
sequence annotation: BBa_K2066524
sequence annotation: BBa_K2066019
sequence: https://synbiohub.org/public/igem/BBa_K2066110_sequence/1

BBa_K2066507
type: Component
persistent identity: component2530759
display id: component2530759
version: 1
top level: BBa_K2066110
definition: BBa_K2066507
access: public

BBa_B0015
type: Component
persistent identity: component2530758
display id: component2530758
version: 1
top level: BBa_K2066110
definition: BBa_B0015
access: public

BBa_B0034
type: Component
persistent identity: component2530762
display id: component2530762
version: 1
top level: BBa_K2066110
definition: BBa_B0034
access: public

BBa_J23101
type: Component
persistent identity: component2530760
display id: component2530760
version: 1
top level: BBa_K2066110
definition: BBa_J23101
access: public

BBa_B0015
type: Component
persistent identity: component2530770
display id: component2530770
version: 1
top level: BBa_K2066110
definition: BBa_B0015
access: public

BBa_K2066524
type: Component
persistent identity: component2530763
display id: component2530763
version: 1
top level: BBa_K2066110
definition: BBa_K2066524
access: public

BBa_K2066019
type: Component
persistent identity: component2530771
display id: component2530771
version: 1
top level: BBa_K2066110
definition: BBa_K2066019
access: public

BBa_K2066018
type: Component
persistent identity: component2530747
display id: component2530747
version: 1
top level: BBa_K2066110
definition: BBa_K2066018
access: public

BBa_K2066506
type: Component
persistent identity: component2530749
display id: component2530749
version: 1
top level: BBa_K2066110
definition: BBa_K2066506
access: public

BBa_K2066508
type: Component
persistent identity: component2530748
display id: component2530748
version: 1
top level: BBa_K2066110
definition: BBa_K2066508
access: public

BBa_K2066509
type: Component
persistent identity: component2530751
display id: component2530751
version: 1
top level: BBa_K2066110
definition: BBa_K2066509
access: public

BBa_K2066510
type: Component
persistent identity: component2530750
display id: component2530750
version: 1
top level: BBa_K2066110
definition: BBa_K2066510
access: public

BBa_K2066018
type: SequenceAnnotation
persistent identity: annotation2530747
display id: annotation2530747
version: 1
top level: BBa_K2066110
location: https://synbiohub.org/public/igem/BBa_K2066110/annotation2530747/range2530747/1
component: BBa_K2066018

https://synbiohub.org/public/igem/BBa_K2066110/annotation2530747/range2530747/1
type: Range
persistent identity: range2530747
display id: range2530747
version: 1
top level: BBa_K2066110
direction: direction/reverse
start: 1
end: 40
orientation: inline

BBa_K2066508
type: SequenceAnnotation
persistent identity: annotation2530748
display id: annotation2530748
version: 1
top level: BBa_K2066110
location: https://synbiohub.org/public/igem/BBa_K2066110/annotation2530748/range2530748/1
component: BBa_K2066508

https://synbiohub.org/public/igem/BBa_K2066110/annotation2530748/range2530748/1
type: Range
persistent identity: range2530748
display id: range2530748
version: 1
top level: BBa_K2066110
direction: direction/reverse
start: 41
end: 118
orientation: inline

BBa_K2066506
type: SequenceAnnotation
persistent identity: annotation2530749
display id: annotation2530749
version: 1
top level: BBa_K2066110
location: https://synbiohub.org/public/igem/BBa_K2066110/annotation2530749/range2530749/1
component: BBa_K2066506

https://synbiohub.org/public/igem/BBa_K2066110/annotation2530749/range2530749/1
type: Range
persistent identity: range2530749
display id: range2530749
version: 1
top level: BBa_K2066110
direction: direction/reverse
start: 119
end: 199
orientation: inline

BBa_K2066510
type: SequenceAnnotation
persistent identity: annotation2530750
display id: annotation2530750
version: 1
top level: BBa_K2066110
location: https://synbiohub.org/public/igem/BBa_K2066110/annotation2530750/range2530750/1
component: BBa_K2066510

https://synbiohub.org/public/igem/BBa_K2066110/annotation2530750/range2530750/1
type: Range
persistent identity: range2530750
display id: range2530750
version: 1
top level: BBa_K2066110
direction: direction/reverse
start: 200
end: 211
orientation: inline

BBa_K2066509
type: SequenceAnnotation
persistent identity: annotation2530751
display id: annotation2530751
version: 1
top level: BBa_K2066110
location: https://synbiohub.org/public/igem/BBa_K2066110/annotation2530751/range2530751/1
component: BBa_K2066509

https://synbiohub.org/public/igem/BBa_K2066110/annotation2530751/range2530751/1
type: Range
persistent identity: range2530751
display id: range2530751
version: 1
top level: BBa_K2066110
direction: direction/reverse
start: 212
end: 931
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2530758
display id: annotation2530758
version: 1
top level: BBa_K2066110
location: https://synbiohub.org/public/igem/BBa_K2066110/annotation2530758/range2530758/1
component: BBa_B0015

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type: Range
persistent identity: range2530758
display id: range2530758
version: 1
top level: BBa_K2066110
direction: direction/reverse
start: 932
end: 1060
orientation: inline

BBa_K2066507
type: SequenceAnnotation
persistent identity: annotation2530759
display id: annotation2530759
version: 1
top level: BBa_K2066110
location: https://synbiohub.org/public/igem/BBa_K2066110/annotation2530759/range2530759/1
component: BBa_K2066507

https://synbiohub.org/public/igem/BBa_K2066110/annotation2530759/range2530759/1
type: Range
persistent identity: range2530759
display id: range2530759
version: 1
top level: BBa_K2066110
direction: direction/reverse
start: 1061
end: 1080
orientation: inline

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2530760
display id: annotation2530760
version: 1
top level: BBa_K2066110
location: https://synbiohub.org/public/igem/BBa_K2066110/annotation2530760/range2530760/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K2066110/annotation2530760/range2530760/1
type: Range
persistent identity: range2530760
display id: range2530760
version: 1
top level: BBa_K2066110
direction: direction/reverse
start: 1081
end: 1115
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2530762
display id: annotation2530762
version: 1
top level: BBa_K2066110
location: https://synbiohub.org/public/igem/BBa_K2066110/annotation2530762/range2530762/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K2066110/annotation2530762/range2530762/1
type: Range
persistent identity: range2530762
display id: range2530762
version: 1
top level: BBa_K2066110
direction: direction/reverse
start: 1116
end: 1127
orientation: inline

BBa_K2066524
type: SequenceAnnotation
persistent identity: annotation2530763
display id: annotation2530763
version: 1
top level: BBa_K2066110
location: https://synbiohub.org/public/igem/BBa_K2066110/annotation2530763/range2530763/1
component: BBa_K2066524

https://synbiohub.org/public/igem/BBa_K2066110/annotation2530763/range2530763/1
type: Range
persistent identity: range2530763
display id: range2530763
version: 1
top level: BBa_K2066110
direction: direction/reverse
start: 1128
end: 2219
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2530770
display id: annotation2530770
version: 1
top level: BBa_K2066110
location: https://synbiohub.org/public/igem/BBa_K2066110/annotation2530770/range2530770/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2066110/annotation2530770/range2530770/1
type: Range
persistent identity: range2530770
display id: range2530770
version: 1
top level: BBa_K2066110
direction: direction/reverse
start: 2220
end: 2348
orientation: inline

BBa_K2066019
type: SequenceAnnotation
persistent identity: annotation2530771
display id: annotation2530771
version: 1
top level: BBa_K2066110
location: https://synbiohub.org/public/igem/BBa_K2066110/annotation2530771/range2530771/1
component: BBa_K2066019

https://synbiohub.org/public/igem/BBa_K2066110/annotation2530771/range2530771/1
type: Range
persistent identity: range2530771
display id: range2530771
version: 1
top level: BBa_K2066110
direction: direction/reverse
start: 2349
end: 2388
orientation: inline

BBa_K2066018
type: ComponentDefinition
persistent identity: BBa K2066018
display id: BBa_K2066018
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 2 Sequence, from Torella et al., 2013
created: 2016-07-11T11:00:00Z
modified: 2016-10-19T05:41:43Z
mutable provenance: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
owned by: james
owned by: myers
mutable description: This is Unique Nucleotide Sequence 2, (UNS 2), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: UNS 2 was chosen because it works well with UNS 3 and it is in accordance with the BioBrick standard.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K2066018_sequence/1

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K2066506
type: ComponentDefinition
persistent identity: BBa K2066506
display id: BBa_K2066506
version: 1
was generated by: iGEM to SBOL conversion
description: RiboJ (Ribozyme Insulator) Lou et. al. 2012
created: 2016-08-30T11:00:00Z
modified: 2016-10-12T12:27:33Z
mutable provenance: Part sequence is from Lou et al. 2012, Supplemental Section V (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???).
owned by: james
owned by: myers
mutable description: RiboJ is the sequence for a ribozyme studied in Lou et. al 2012 ("Ribozyme-based insulator parts buffer synthetic circuits from genetic context"). WM iGEM 2016 used this sequence between the promoter and ribosome sequence. One of our goals for using this part is moving it onto a Biobrick backbone. Furthermore, In Lou et. al, this ribozyme sequence was said to act as an insulator which generalizes protein expression levels for a given promoter. We used RiboJ to collect data for our Ribozyme characterization project as well as our ribosome and promoter characterization projects.
discontinued: false
dominant: false
group u list: _2534_
m user id: 27446
owner id: 31541
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: We designed this part to use as an insulator and also move this riboJ sequence onto a Biobrick backbone.
star: false
creator: Likhitha Kolla
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_K2066506_sequence/1

BBa_K2066507
type: ComponentDefinition
persistent identity: BBa K2066507
display id: BBa_K2066507
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 6.1 (Torella et. al 2013)
created: 2016-08-30T11:00:00Z
modified: 2016-10-19T06:10:34Z
mutable provenance: UNS 6.1 sequence derived from (Torella et. al 2013) "Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly".
owned by: james
owned by: myers
mutable description: This part is used as a spacer. WM iGEM 2016 used this part as a spacer to connect two separate composite parts onto the same plasmid. For example, UNS 6.1 was used as a spacer to connect K2066022(TetR on UNS) and K2066023(pTET GFP on UNS) to make K2066053.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 31541
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Used this part as a spacer.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K2066507_sequence/1

BBa_K2066508
type: ComponentDefinition
persistent identity: BBa K2066508
display id: BBa_K2066508
version: 1
was generated by: iGEM to SBOL conversion
description: Modified pLacO-1 Promoter (Lou et. al 2012)
created: 2016-08-30T11:00:00Z
modified: 2016-08-31T09:01:04Z
mutable provenance: Part sequence inspired by Lou et al. 2012 (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???)
owned by: james
owned by: myers
mutable description: This promoter sequence is modified from section V of Supplementary Material of Lou et al. The Supplementary sequence contains 98bp of the end of BioBrick backbone pSB1C3, followed by an EcoRI site and an XbaI site, then 20bp of the beginning of pTac (as described in fig. S1), before beginning the sequence of plLacO-1 (as described in fig. S1). Here we use only the 20bp of pTac followed by the plLacO-1 sequence as described in fig. S1. WM iGEM 2016 used this part for our Ribozyme Characterization project.
discontinued: false
dominant: false
group u list: _2534_
m user id: 31541
owner id: 31541
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Design inspired by Lou et al. 2012 (???Ribozyme-based insulator parts buffer synthetic circuits from genetic context???) so that constitutive LacI repressor can bind to it.
star: false
creator: Likhitha Kolla
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_K2066508_sequence/1

BBa_K2066509
type: ComponentDefinition
persistent identity: BBa K2066509
display id: BBa_K2066509
version: 1
was generated by: iGEM to SBOL conversion
description: sfGFP
created: 2016-08-30T11:00:00Z
modified: 2016-10-19T02:54:25Z
mutable provenance: The sequence for this sfGFP reporter gene is modified from Lou et al. Supplement section V. This is the sequence of superfolder GFP BBa_I746916, but with four codon modifications to match WM16_015: at position 441, G->T. At 446, C->T. At 495, T->C. At 562, C->A.
owned by: james
owned by: myers
mutable description: The part is a reporter used for K2066014.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 31541
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Design inspired by Lou et. al. 2012
star: false
creator: Likhitha Kolla
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence: https://synbiohub.org/public/igem/BBa_K2066509_sequence/1

BBa_K2066510
type: ComponentDefinition
persistent identity: BBa K2066510
display id: BBa_K2066510
version: 1
was generated by: iGEM to SBOL conversion
description: Strong RBS from Lou et. al 2012
created: 2016-08-30T11:00:00Z
modified: 2016-08-31T09:32:28Z
mutable provenance: Sequence is from from Lou et al. Supplement section V.
owned by: james
owned by: myers
mutable description: This part is a strong RBS from Lou et. al 2012 "Ribozyme-based insulator parts buffer synthetic circuits from genetic context". The RBS is used to make some of 2016 WM iGEM Ribozyme Characterization project parts.
discontinued: false
dominant: false
group u list: _2534_
m user id: 31541
owner id: 31541
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: RBS sequence from Lou et. al 2012
star: false
creator: Likhitha Kolla
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K2066510_sequence/1

BBa_K2066019
type: ComponentDefinition
persistent identity: BBa K2066019
display id: BBa_K2066019
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 3 Sequence, from Torella et al., 2013
created: 2016-07-11T11:00:00Z
modified: 2016-10-19T05:43:00Z
mutable provenance: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
owned by: james
owned by: myers
mutable description: This is Unique Nucleotide Sequence 3, (UNS 3), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki. The sequence for this part came from the following paper: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860. A huge thanks to all the researchers involved in its original creation!
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: This UNS sequence was chosen to serve as the 3' primer in our standard because it works well with UNS 2 and it adheres to the BioBrick standards.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066019_sequence/1

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

BBa_K2066524
type: ComponentDefinition
persistent identity: BBa K2066524
display id: BBa_K2066524
version: 1
was generated by: iGEM to SBOL conversion
description: LacI no LVA
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T10:09:36Z
mutable provenance: Derived from BBa_C0012.
owned by: james
owned by: myers
mutable description: This is a modified LacI repressor (C0012) with the LVA tail removed. This basic part contains no RBS.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31526
owner id: 31526
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Translated the C0012 sequence and selected everything before the coding sequence for LVA, which is AANDENYALVA. I then added the stop codon tga to the end of the truncated C0012 sequence
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2066524_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K2066110_sequence/1
type: Sequence
persistent identity: BBa K2066110 sequence
display id: BBa_K2066110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacggaaacaaacgcagaatccaagcggcaaatattctgaaatgagctgataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcacagctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaaactagaaggaggaaaaaaatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtgtacattaccgcagataaacaaaaaaatggcattaaagcgaatttcaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatactcgttcgctgccacctaagtttacagctagctcagtcctaggtattatgctagcaaagaggagaaaatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccacc…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066018_sequence/1
type: Sequence
persistent identity: BBa K2066018 sequence
display id: BBa_K2066018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacggaaacaaacgcagaatccaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066506_sequence/1
type: Sequence
persistent identity: BBa K2066506 sequence
display id: BBa_K2066506_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctgtcaccggatgtgctttccggtctgatgagtccgtgaggacgaaacagcctctacaaataattttgtttaaactaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066507_sequence/1
type: Sequence
persistent identity: BBa K2066507 sequence
display id: BBa_K2066507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcgttcgctgccacctaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066508_sequence/1
type: Sequence
persistent identity: BBa K2066508 sequence
display id: BBa_K2066508_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcaaatattctgaaatgagctgataaatgtgagcggataacattgacattgtgagcggataacaagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066019_sequence/1
type: Sequence
persistent identity: BBa K2066019 sequence
display id: BBa_K2066019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcactgaaggtcctcaatcgcactggaaacatcaaggtcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066509_sequence/1
type: Sequence
persistent identity: BBa K2066509 sequence
display id: BBa_K2066509_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtgtacattaccgcagataaacaaaaaaatggcattaaagcgaatttcaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066510_sequence/1
type: Sequence
persistent identity: BBa K2066510 sequence
display id: BBa_K2066510_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aggaggaaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066524_sequence/1
type: Sequence
persistent identity: BBa K2066524 sequence
display id: BBa_K2066524_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z