LOCUS       BBa_K2066111            2404 bp    DNA     linear       22-Dec-2024
DEFINITION  pLacO1 sfGFP + LacI (weak)
ACCESSION   BBa_K2066111
VERSION     BBa_K2066111.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     promoter        41..118
                     /label=BBa_K2066508
     misc_feature    119..199
                     /label=BBa_K2066506
     RBS             200..211
                     /label=BBa_K2066510
     misc_feature    212..931
                     /label=BBa_K2066509
     stem_loop       943..986
                     /label=stem_loop
     misc_feature    932..1011
                     /label=BBa_B0010
     terminator      932..1011
                     /label=BBa_B0010
     stem_loop       1027..1046
                     /label=T7 TE
     misc_feature    1053..1053
                     /label=stop
     polyA_site      1047..1060
                     /label=polya
     misc_feature    1020..1060
                     /label=BBa_B0012
     terminator      1020..1060
                     /label=BBa_B0012
     terminator      932..1060
                     /label=BBa_B0015
     misc_feature    1061..1080
                     /label=BBa_K2066507
     promoter        1081..1115
                     /label=BBa_J23101
     misc_feature    1116..1123
                     /label=BBa_K2066529
     misc_feature    1130..1133
                     /label=conserved
     RBS             1124..1137
                     /label=BBa_B0031
     misc_feature    1138..1143
                     /label=BBa_K2066530
     CDS             1144..2235
                     /label=BBa_K2066524
     stem_loop       2247..2290
                     /label=stem_loop
     misc_feature    2236..2315
                     /label=BBa_B0010
     terminator      2236..2315
                     /label=BBa_B0010
     stem_loop       2331..2350
                     /label=T7 TE
     misc_feature    2357..2357
                     /label=stop
     polyA_site      2351..2364
                     /label=polya
     misc_feature    2324..2364
                     /label=BBa_B0012
     terminator      2324..2364
                     /label=BBa_B0012
     terminator      2236..2364
                     /label=BBa_B0015
     misc_feature    2365..2404
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc ggcaaatatt ctgaaatgag
       61 ctgataaatg tgagcggata acattgacat tgtgagcgga taacaagata ctgagcacag
      121 ctgtcaccgg atgtgctttc cggtctgatg agtccgtgag gacgaaacag cctctacaaa
      181 taattttgtt taaactagaa ggaggaaaaa aatgcgtaaa ggcgaagagc tgttcactgg
      241 tgtcgtccct attctggtgg aactggatgg tgatgtcaac ggtcataagt tttccgtgcg
      301 tggcgagggt gaaggtgacg caactaatgg taaactgacg ctgaagttca tctgtactac
      361 tggtaaactg ccggtacctt ggccgactct ggtaacgacg ctgacttatg gtgttcagtg
      421 ctttgctcgt tatccggacc atatgaagca gcatgacttc ttcaagtccg ccatgccgga
      481 aggctatgtg caggaacgca cgatttcctt taaggatgac ggcacgtaca aaacgcgtgc
      541 ggaagtgaaa tttgaaggcg ataccctggt aaaccgcatt gagctgaaag gcattgactt
      601 taaagaagac ggcaatatcc tgggccataa gctggaatac aattttaaca gccacaatgt
      661 gtacattacc gcagataaac aaaaaaatgg cattaaagcg aatttcaaaa ttcgccacaa
      721 cgtggaggat ggcagcgtgc agctggctga tcactaccag caaaacactc caatcggtga
      781 tggtcctgtt ctgctgccag acaatcacta tctgagcacg caaagcgttc tgtctaaaga
      841 tccgaacgag aaacgcgatc atatggttct gctggagttc gtaaccgcag cgggcatcac
      901 gcatggtatg gatgaactgt acaaatgatg accaggcatc aaataaaacg aaaggctcag
      961 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1021 cacactggct caccttcggg tgggcctttc tgcgtttata ctcgttcgct gccacctaag
     1081 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaacctac
     1141 tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct
     1201 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa
     1261 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg
     1321 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg
     1381 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg
     1441 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg
     1501 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg
     1561 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc
     1621 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca
     1681 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg
     1741 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg
     1801 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc
     1861 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc
     1921 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc
     1981 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg
     2041 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat
     2101 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc
     2161 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     2221 gaaagcgggc agtgaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     2281 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     2341 cgggtgggcc tttctgcgtt tatagcactg aaggtcctca atcgcactgg aaacatcaag
     2401 gtcg
//