LOCUS       BBa_K2066113            2748 bp    DNA     linear       16-Jun-2024
DEFINITION  Synthetic Enhancer with 2X TetO cassette (55as) on UNS backbone
ACCESSION   BBa_K2066113
VERSION     BBa_K2066113.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     misc_feature    41..87
                     /label=BBa_K2066543
     misc_feature    88..175
                     /label=BBa_K2066532
     promoter        176..205
                     /label=BBa_K2066544
     misc_feature    206..227
                     /label=BBa_K2066545
     misc_feature    228..370
                     /label=BBa_K2066547
     CDS             372..1865
                     /label=BBa_K2066546
     misc_feature    1866..2579
                     /label=BBa_K2066518
     stem_loop       2591..2634
                     /label=stem_loop
     misc_feature    2580..2659
                     /label=BBa_B0010
     terminator      2580..2659
                     /label=BBa_B0010
     stem_loop       2675..2694
                     /label=T7 TE
     misc_feature    2701..2701
                     /label=stop
     polyA_site      2695..2708
                     /label=polya
     misc_feature    2668..2708
                     /label=BBa_B0012
     terminator      2668..2708
                     /label=BBa_B0012
     terminator      2580..2708
                     /label=BBa_B0015
     misc_feature    2709..2748
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc tcacaattgc accaacatgg
       61 tgcttaatgt ttccattgaa gcactatatt ggtgcaacat tgtcttcgac gctagcagcg
      121 cgcggagatt ctagcccctt ttcgaggaac gtccccatga taacgccttt taggggcaat
      181 ttaaaagttg gcacagattt cgctttatct tttttaattg tgagcgctca caattaagct
      241 tggcgaaggt ggcaccgggc ttggcttatc catcgctcgt aatttgattg atcagcattc
      301 aggcaaaatt gaatttacca gttggccagg gcataccgag ttctcggttt acctgcctat
      361 caggaaataa acgtttatgc aacgagggat agtctgggta gtcgatgacg atagttccat
      421 ccgttgggtg cttgaacgtg cgctcgctgg ggcaggttta acctgtacga cgtttgagaa
      481 cggcgcagaa gtgctggagg cgctggcgag caaaacgccg gatgtgctgc tttcagatat
      541 ccgtatgccg ggaatggacg ggctggcgct gctcaagcag attaaacagc gccatccaat
      601 gcttccggtc atcattatga ccgcacattc cgatctggat gctgccgtca gcgcctatca
      661 acaaggggcg tttgattatc tgcccaaacc gtttgatatc gacgaagcag tggcgctggt
      721 tgagcgcgct atcagtcatt accaggaaca gcagcagccg cgtaatgttc agcttaacgg
      781 cccaacgacc gatatcatcg gcgaagcgcc agccatgcag gacgtgttcc gtattatcgg
      841 tcggctttcg cgttcttcta ttagcgtgct gattaacggc gaatccggca ccggtaaaga
      901 actggtcgct catgccctgc atcgccacag tccgcgcgcc aaagcgccgt ttatcgcgct
      961 gaatatggca gctatcccaa aagatttgat cgaatcagaa ctgtttggcc acgagaaagg
     1021 cgcgtttact ggcgcgaata ccattcgtca ggggcgtttt gaacaggccg atggcggtac
     1081 attattcctc gacgaaattg gtgatatgcc gctggatgtg cagacgcgtt tgctgcgcgt
     1141 gctggcagac ggtcagtttt accgcgttgg cggctatgcg ccggtgaaag tggatgtgcg
     1201 gattatcgct gccactcacc agaatctcga acagcgagtg caggaaggta agttccgtga
     1261 ggatctgttc caccgcctga acgttatccg cgttcatctg ccgccgctgc gcgaacgtcg
     1321 ggaagatatt ccccgtctgg cgcgccattt tttacaggtt gccgcgcgcg aactgggcgt
     1381 agaagcgaag ttactgcatc cggaaaccga agctgctctg acgcgtctgg cgtggccagg
     1441 caacgtgcgc cagctggaaa acacctgccg ctggctaacg gtgatggccg ccgggcagga
     1501 agtgttgatt caggatttgc ccggcgaact gtttgaatca acggttgcgg agagtacttc
     1561 gcaaatgcaa ccggacagct gggcgacgct tcttgcgcag tgggcagaca gagcgctgcg
     1621 ttccggtcat caaaatctgc tttccgaagc gcagccagag ctggagcgga cgttactgac
     1681 gaccgcgttg cgacatacgc aggggcataa acaggaagcg gcgcggctac tcggctgggg
     1741 ccgcaacacc ctgacgcgta agttaaaaga gctggggatg gagtgattca cagcttgtgt
     1801 gtaaagattg attattgagc gcaaattgct ggtattttac gctttactgt tccaggagga
     1861 aaaaaatggt gagcaagggc gaagaagata acatggccat catcaaggag ttcatgcgct
     1921 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     1981 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     2041 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     2101 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     2161 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccttgc
     2221 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     2281 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     2341 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     2401 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     2461 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     2521 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaataac
     2581 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2641 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2701 cgtttatagc actgaaggtc ctcaatcgca ctggaaacat caaggtcg
//