BBa_K2066114
type: ComponentDefinition
persistent identity: BBa K2066114
display id: BBa_K2066114
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer: 3x TetO Binding Cassette (52s) + NRII on UNS
created: 2016-10-13T11:00:00Z
modified: 2016-10-28T08:24:09Z
mutable provenance: To be completed
owned by: james
owned by: myers
mutable description: To be completed
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 31547
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: To be completed
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K2066018
component: BBa_K2066531
component: BBa_K2066543
component: BBa_K2066545
component: BBa_K2066544
component: BBa_K2066546
component: BBa_K2066547
component: BBa_B0015
component: BBa_K2066518
component: BBa_K2066539
component: BBa_K2066538
component: BBa_K2066126
component: BBa_K2066540
sequence annotation: BBa_K2066018
sequence annotation: BBa_K2066543
sequence annotation: BBa_K2066531
sequence annotation: BBa_K2066544
sequence annotation: BBa_K2066545
sequence annotation: BBa_K2066547
sequence annotation: BBa_K2066546
sequence annotation: BBa_K2066518
sequence annotation: BBa_B0015
sequence annotation: BBa_K2066539
sequence annotation: BBa_K2066126
sequence annotation: BBa_K2066538
sequence annotation: BBa_K2066540
sequence: https://synbiohub.org/public/igem/BBa_K2066114_sequence/1

BBa_K2066018
type: Component
persistent identity: component2523564
display id: component2523564
version: 1
top level: BBa_K2066114
definition: BBa_K2066018
access: public

BBa_K2066531
type: Component
persistent identity: component2523566
display id: component2523566
version: 1
top level: BBa_K2066114
definition: BBa_K2066531
access: public

BBa_K2066543
type: Component
persistent identity: component2523565
display id: component2523565
version: 1
top level: BBa_K2066114
definition: BBa_K2066543
access: public

BBa_K2066545
type: Component
persistent identity: component2523568
display id: component2523568
version: 1
top level: BBa_K2066114
definition: BBa_K2066545
access: public

BBa_K2066544
type: Component
persistent identity: component2523567
display id: component2523567
version: 1
top level: BBa_K2066114
definition: BBa_K2066544
access: public

BBa_K2066546
type: Component
persistent identity: component2523570
display id: component2523570
version: 1
top level: BBa_K2066114
definition: BBa_K2066546
access: public

BBa_K2066547
type: Component
persistent identity: component2523569
display id: component2523569
version: 1
top level: BBa_K2066114
definition: BBa_K2066547
access: public

BBa_B0015
type: Component
persistent identity: component2523578
display id: component2523578
version: 1
top level: BBa_K2066114
definition: BBa_B0015
access: public

BBa_K2066518
type: Component
persistent identity: component2523571
display id: component2523571
version: 1
top level: BBa_K2066114
definition: BBa_K2066518
access: public

BBa_K2066018
type: Component
persistent identity: component2523580
display id: component2523580
version: 1
top level: BBa_K2066114
definition: BBa_K2066018
access: public

BBa_K2066539
type: Component
persistent identity: component2523579
display id: component2523579
version: 1
top level: BBa_K2066114
definition: BBa_K2066539
access: public

BBa_K2066538
type: Component
persistent identity: component2523582
display id: component2523582
version: 1
top level: BBa_K2066114
definition: BBa_K2066538
access: public

BBa_K2066126
type: Component
persistent identity: component2523581
display id: component2523581
version: 1
top level: BBa_K2066114
definition: BBa_K2066126
access: public

BBa_K2066540
type: Component
persistent identity: component2523590
display id: component2523590
version: 1
top level: BBa_K2066114
definition: BBa_K2066540
access: public

BBa_B0015
type: Component
persistent identity: component2523589
display id: component2523589
version: 1
top level: BBa_K2066114
definition: BBa_B0015
access: public

BBa_K2066018
type: SequenceAnnotation
persistent identity: annotation2523564
display id: annotation2523564
version: 1
top level: BBa_K2066114
location: https://synbiohub.org/public/igem/BBa_K2066114/annotation2523564/range2523564/1
component: BBa_K2066018

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type: Range
persistent identity: range2523564
display id: range2523564
version: 1
top level: BBa_K2066114
direction: direction/reverse
start: 1
end: 40
orientation: inline

BBa_K2066543
type: SequenceAnnotation
persistent identity: annotation2523565
display id: annotation2523565
version: 1
top level: BBa_K2066114
location: https://synbiohub.org/public/igem/BBa_K2066114/annotation2523565/range2523565/1
component: BBa_K2066543

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type: Range
persistent identity: range2523565
display id: range2523565
version: 1
top level: BBa_K2066114
direction: direction/reverse
start: 41
end: 87
orientation: inline

BBa_K2066531
type: SequenceAnnotation
persistent identity: annotation2523566
display id: annotation2523566
version: 1
top level: BBa_K2066114
location: https://synbiohub.org/public/igem/BBa_K2066114/annotation2523566/range2523566/1
component: BBa_K2066531

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type: Range
persistent identity: range2523566
display id: range2523566
version: 1
top level: BBa_K2066114
direction: direction/reverse
start: 88
end: 236
orientation: inline

BBa_K2066544
type: SequenceAnnotation
persistent identity: annotation2523567
display id: annotation2523567
version: 1
top level: BBa_K2066114
location: https://synbiohub.org/public/igem/BBa_K2066114/annotation2523567/range2523567/1
component: BBa_K2066544

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type: Range
persistent identity: range2523567
display id: range2523567
version: 1
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direction: direction/reverse
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orientation: inline

BBa_K2066545
type: SequenceAnnotation
persistent identity: annotation2523568
display id: annotation2523568
version: 1
top level: BBa_K2066114
location: https://synbiohub.org/public/igem/BBa_K2066114/annotation2523568/range2523568/1
component: BBa_K2066545

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type: Range
persistent identity: range2523568
display id: range2523568
version: 1
top level: BBa_K2066114
direction: direction/reverse
start: 267
end: 288
orientation: inline

BBa_K2066547
type: SequenceAnnotation
persistent identity: annotation2523569
display id: annotation2523569
version: 1
top level: BBa_K2066114
location: https://synbiohub.org/public/igem/BBa_K2066114/annotation2523569/range2523569/1
component: BBa_K2066547

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type: Range
persistent identity: range2523569
display id: range2523569
version: 1
top level: BBa_K2066114
direction: direction/reverse
start: 289
end: 431
orientation: inline

BBa_K2066546
type: SequenceAnnotation
persistent identity: annotation2523570
display id: annotation2523570
version: 1
top level: BBa_K2066114
location: https://synbiohub.org/public/igem/BBa_K2066114/annotation2523570/range2523570/1
component: BBa_K2066546

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type: Range
persistent identity: range2523570
display id: range2523570
version: 1
top level: BBa_K2066114
direction: direction/reverse
start: 433
end: 1926
orientation: inline

BBa_K2066518
type: SequenceAnnotation
persistent identity: annotation2523571
display id: annotation2523571
version: 1
top level: BBa_K2066114
location: https://synbiohub.org/public/igem/BBa_K2066114/annotation2523571/range2523571/1
component: BBa_K2066518

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type: Range
persistent identity: range2523571
display id: range2523571
version: 1
top level: BBa_K2066114
direction: direction/reverse
start: 1927
end: 2640
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2523578
display id: annotation2523578
version: 1
top level: BBa_K2066114
location: https://synbiohub.org/public/igem/BBa_K2066114/annotation2523578/range2523578/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2066114/annotation2523578/range2523578/1
type: Range
persistent identity: range2523578
display id: range2523578
version: 1
top level: BBa_K2066114
direction: direction/reverse
start: 2641
end: 2769
orientation: inline

BBa_K2066539
type: SequenceAnnotation
persistent identity: annotation2523579
display id: annotation2523579
version: 1
top level: BBa_K2066114
location: https://synbiohub.org/public/igem/BBa_K2066114/annotation2523579/range2523579/1
component: BBa_K2066539

https://synbiohub.org/public/igem/BBa_K2066114/annotation2523579/range2523579/1
type: Range
persistent identity: range2523579
display id: range2523579
version: 1
top level: BBa_K2066114
direction: direction/reverse
start: 2770
end: 2789
orientation: inline

BBa_K2066126
type: SequenceAnnotation
persistent identity: annotation2523581
display id: annotation2523581
version: 1
top level: BBa_K2066114
location: https://synbiohub.org/public/igem/BBa_K2066114/annotation2523581/range2523581/1
component: BBa_K2066126

https://synbiohub.org/public/igem/BBa_K2066114/annotation2523581/range2523581/1
type: Range
persistent identity: range2523581
display id: range2523581
version: 1
top level: BBa_K2066114
direction: direction/reverse
start: 2790
end: 2856
orientation: inline

BBa_K2066018
type: SequenceAnnotation
persistent identity: annotation2523580
display id: annotation2523580
version: 1
top level: BBa_K2066114
location: https://synbiohub.org/public/igem/BBa_K2066114/annotation2523580/range2523580/1
component: BBa_K2066018

https://synbiohub.org/public/igem/BBa_K2066114/annotation2523580/range2523580/1
type: Range
persistent identity: range2523580
display id: range2523580
version: 1
top level: BBa_K2066114
direction: direction/reverse
start: 2790
end: 2829
orientation: inline

BBa_K2066538
type: SequenceAnnotation
persistent identity: annotation2523582
display id: annotation2523582
version: 1
top level: BBa_K2066114
location: https://synbiohub.org/public/igem/BBa_K2066114/annotation2523582/range2523582/1
component: BBa_K2066538

https://synbiohub.org/public/igem/BBa_K2066114/annotation2523582/range2523582/1
type: Range
persistent identity: range2523582
display id: range2523582
version: 1
top level: BBa_K2066114
direction: direction/reverse
start: 2857
end: 3906
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2523589
display id: annotation2523589
version: 1
top level: BBa_K2066114
location: https://synbiohub.org/public/igem/BBa_K2066114/annotation2523589/range2523589/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2066114/annotation2523589/range2523589/1
type: Range
persistent identity: range2523589
display id: range2523589
version: 1
top level: BBa_K2066114
direction: direction/reverse
start: 3907
end: 4035
orientation: inline

BBa_K2066540
type: SequenceAnnotation
persistent identity: annotation2523590
display id: annotation2523590
version: 1
top level: BBa_K2066114
location: https://synbiohub.org/public/igem/BBa_K2066114/annotation2523590/range2523590/1
component: BBa_K2066540

https://synbiohub.org/public/igem/BBa_K2066114/annotation2523590/range2523590/1
type: Range
persistent identity: range2523590
display id: range2523590
version: 1
top level: BBa_K2066114
direction: direction/reverse
start: 4036
end: 4055
orientation: inline

BBa_K2066018
type: ComponentDefinition
persistent identity: BBa K2066018
display id: BBa_K2066018
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 2 Sequence, from Torella et al., 2013
created: 2016-07-11T11:00:00Z
modified: 2016-10-19T05:41:43Z
mutable provenance: Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860.
owned by: james
owned by: myers
mutable description: This is Unique Nucleotide Sequence 2, (UNS 2), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27446
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: UNS 2 was chosen because it works well with UNS 3 and it is in accordance with the BioBrick standard.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K2066018_sequence/1

BBa_K2066531
type: ComponentDefinition
persistent identity: BBa K2066531
display id: BBa_K2066531
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: 3X TetO Binding Cassette(52S)
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:28:59Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: .
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066531_sequence/1

BBa_K2066543
type: ComponentDefinition
persistent identity: BBa K2066543
display id: BBa_K2066543
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: Enhancer/NRI binding sites
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T04:20:03Z
mutable provenance: mep
owned by: james
owned by: myers
mutable description: tmept
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: temp
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066543_sequence/1

BBa_K2066544
type: ComponentDefinition
persistent identity: BBa K2066544
display id: BBa_K2066544
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: glnAp2 promoter
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T04:48:44Z
mutable provenance: temp
owned by: james
owned by: myers
mutable description: temp
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: temp
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K2066544_sequence/1

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K2066518
type: ComponentDefinition
persistent identity: BBa K2066518
display id: BBa_K2066518
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry (with start codon)
created: 2016-10-07T11:00:00Z
modified: 2016-10-08T10:50:28Z
owned by: james
owned by: myers
mutable description: Modified from the mCherry used in K2066028 and K2066029 to include the start codon. This modified mCherry is used in the construction of K2066040 and K2066041.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 31544
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The start codon was removed in K2066028 and K2066029 to follow the design from Synthetic Analog Computation in Living Cells (Daniel et al.) where the codon bias was being optimized.
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence: https://synbiohub.org/public/igem/BBa_K2066518_sequence/1

BBa_K2066126
type: ComponentDefinition
persistent identity: BBa K2066126
display id: BBa_K2066126
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: NRII2302 Upstream Regulatory Region with Promoter
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:55:12Z
mutable provenance: temp
owned by: james
owned by: myers
mutable description: temp
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: temp
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_K2066018
sequence: https://synbiohub.org/public/igem/BBa_K2066126_sequence/1

BBa_K2066018
type: SequenceAnnotation
persistent identity: annotation2500695
display id: annotation2500695
version: 1
top level: BBa_K2066126
location: https://synbiohub.org/public/igem/BBa_K2066126/annotation2500695/range2500695/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K2066126/annotation2500695/range2500695/1
type: Range
persistent identity: range2500695
display id: range2500695
version: 1
top level: BBa_K2066126
direction: direction/reverse
start: 1
end: 40
orientation: inline

BBa_K2066538
type: ComponentDefinition
persistent identity: BBa K2066538
display id: BBa_K2066538
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: NRII2302 Mutant Coding
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:51:49Z
mutable provenance: te
owned by: james
owned by: myers
mutable description: temp
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: mptem
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K2066538_sequence/1

BBa_K2066539
type: ComponentDefinition
persistent identity: BBa K2066539
display id: BBa_K2066539
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 3.1
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T02:36:08Z
mutable provenance: temp
owned by: james
owned by: myers
mutable description: temp
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: tme
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066539_sequence/1

BBa_K2066540
type: ComponentDefinition
persistent identity: BBa K2066540
display id: BBa_K2066540
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 3.2
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T06:23:08Z
mutable provenance: tem
owned by: james
owned by: myers
mutable description: temp
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: p
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066540_sequence/1

BBa_K2066545
type: ComponentDefinition
persistent identity: BBa K2066545
display id: BBa_K2066545
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: LacI binding site (LacO)
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:05:36Z
mutable provenance: temp
owned by: james
owned by: myers
mutable description: temp
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: tem
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K2066545_sequence/1

BBa_K2066546
type: ComponentDefinition
persistent identity: BBa K2066546
display id: BBa_K2066546
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: glnG (NRI)
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:07:26Z
mutable provenance: mept
owned by: james
owned by: myers
mutable description: tempt
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: metp
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2066546_sequence/1

BBa_K2066547
type: ComponentDefinition
persistent identity: BBa K2066547
display id: BBa_K2066547
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: Spacer between Promoter and RBS
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:11:55Z
mutable provenance: petm
owned by: james
owned by: myers
mutable description: ptem
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: ep
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066547_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K2066114_sequence/1
type: Sequence
persistent identity: BBa K2066114 sequence
display id: BBa_K2066114_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacggaaacaaacgcagaatccaagctcacaattgcaccaacatggtgcttaatgtttccattgaagcactatattggtgcaacattgtcttcgacgctagctctctatcactgatagggatgtcggaggctcgcggactctatcaatgatagagtctcgagtctggccggatctctatcactgatagggagctagcgtccccatgataacgccttttaggggcaatttaaaagttggcacagatttcgctttatcttttttaattgtgagcgctcacaattaagcttggcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataaacgtttatgcaacgagggatagtctgggtagtcgatgacgatagttccatccgttgggtgcttgaacgtgcgctcgctggggcaggtttaacctgtacgacgtttgagaacggcgcagaagtgctggaggcgctggcgagcaaaacgccggatgtgctgctttcagatatccgtatgccgggaatggacgggctggcgctgctcaagcagattaaacagcgccatccaatgcttccggtcatcattatgaccgcacattccgatctggatgctgccgtcagcgcctatcaacaaggggcgtttgattatctgcccaaaccgtttgatatcgacgaagcagtggcgctggttgagcgcgctatcagtcattaccaggaacagcagcagccgcgtaatgttcagcttaacggcccaacgaccgatatcatcggcgaagcgccagccatgcaggacgtgttccgtattatcggtcggctttcgcgttcttctattagcgtgctgattaacggcgaatccggcaccggtaaagaactggtcgctcatgccctgcatcgccacagtccgcgcgccaaagcgccgtttatcgcgctgaatatggcagctatcccaaaagatttgatcgaatcagaactgtttggccacgagaaaggcgcgtttactggcgcgaataccattcgtcaggggcgttttgaacaggccgatggcggtacattattcctcgacgaaattggtgatatgccgctggatgtgcagacgcgtttgctgcgcgtgctggcagacggtcagttttaccgcgttggcggctatgcgccggtgaaagtggatgtgcggattatcgctgccactcaccagaatctcgaacagcgagtgcaggaaggtaagttccgtgaggatctgttccaccgcctgaacgttatccgcgttcatctgccgccgctgcgcgaacgtcgggaagatattccccgtctggcgcgccattttttacaggttgccgcgcgcgaactgggcgtagaagcgaagttactgcatccggaaaccgaagctgctctgacgcgtctggcgtggccaggcaacgtgcgccagctggaaaacacctgccgctggctaacggtgatggccgccgggcaggaagtgttgattcaggatttgcccggcgaactgtttgaatcaacggttgcggagagtacttcgcaaatgcaaccggacagctgggcgacgcttcttgcgcagtgggcagacagagcgctgcgttccggtcatcaaaatctgctttccgaagcgcagccagagctggagcggacgttactgacgaccgcgttgcgacatacgcaggggcataaacaggaagcggcgcggctactcggctggggccgcaacaccctgacgcgtaagttaaaagagctggggatggagtgattcacagcttgtgtgtaaagattgattattgagcgcaaattgctggtattttacgctttactgttccaggaggaaaaaaatggtgagcaagggcgaagaagataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggaggg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066018_sequence/1
type: Sequence
persistent identity: BBa K2066018 sequence
display id: BBa_K2066018_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacggaaacaaacgcagaatccaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066518_sequence/1
type: Sequence
persistent identity: BBa K2066518 sequence
display id: BBa_K2066518_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtgagcaagggcgaagaagataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066126_sequence/1
type: Sequence
persistent identity: BBa K2066126 sequence
display id: BBa_K2066126_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtaggccggagcaggtgagtcgctctccaacgtgaagtttgtcagctatctgtagcccatctctgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066531_sequence/1
type: Sequence
persistent identity: BBa K2066531 sequence
display id: BBa_K2066531_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attggtgcaacattgtcttcgacgctagctctctatcactgatagggatgtcggaggctcgcggactctatcaatgatagagtctcgagtctggccggatctctatcactgatagggagctagcgtccccatgataacgccttttaggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066538_sequence/1
type: Sequence
persistent identity: BBa K2066538 sequence
display id: BBa_K2066538_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcaacaggcacgcagcccgatgctgggcagatcctcaactcgctgattaacagtattttgttaatcgatgacaacctggcgatccattacgccaaccctgccgcgcaacaactgctcgcccaaagctcccgcaaattgtttggtacaccgttaccggaactgttgagctacttctcattaaatatcgagctgatgcaagaaagtctggaggcggggcaaggttttaccgataacgaagtgacgctggtcatcgacgggcgctcgcatatcctttctgtgacggcccagcgtatgccggacggcatgatcctgctggagatggctccgatggataaccagcgccgcttaagtcaggaacagctacagcacgcccagcaggttactgcccgtgatttagtgcgcggcctggcacatgagattaaaaatccgcttggcggtttacgtggcgcggcgcagctgctcagcaaagcgttacctgacccatcactactcgaatataccaaagtgattatcgaacaggcggaccggctgcgaaatctggtcgaccgtctgttggggccgcagctgcccggtacgcgcgttaccgaaagtattcacaaagtggctgaacgcgtggtaacgctggtgtcgatggaactgccggacaacgtgcggttgattcgtgattacgatcccagcctaccggaactggcgcacgacccggatcaaattgaacaggtcttgctgaatattgtgcgcaatgcgctacaggcgctggggccggaaggcggtgaaatcattctgcgtacccgcaccgcgtttcaactgaccttacacggcgagcgctaccggctggcggcgcggattgatgtggaagataacgggccgggcattccgcctcatttgcaggatacgctgttttacccgatggtcagcggccgcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066539_sequence/1
type: Sequence
persistent identity: BBa K2066539 sequence
display id: BBa_K2066539_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcactgaaggtcctcaatcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066540_sequence/1
type: Sequence
persistent identity: BBa K2066540 sequence
display id: BBa_K2066540_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cactggaaacatcaaggtcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066543_sequence/1
type: Sequence
persistent identity: BBa K2066543 sequence
display id: BBa_K2066543_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacaattgcaccaacatggtgcttaatgtttccattgaagcactat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066544_sequence/1
type: Sequence
persistent identity: BBa K2066544 sequence
display id: BBa_K2066544_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaatttaaaagttggcacagatttcgctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066545_sequence/1
type: Sequence
persistent identity: BBa K2066545 sequence
display id: BBa_K2066545_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tatcttttttaattgtgagcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066546_sequence/1
type: Sequence
persistent identity: BBa K2066546 sequence
display id: BBa_K2066546_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtttatgcaacgagggatagtctgggtagtcgatgacgatagttccatccgttgggtgcttgaacgtgcgctcgctggggcaggtttaacctgtacgacgtttgagaacggcgcagaagtgctggaggcgctggcgagcaaaacgccggatgtgctgctttcagatatccgtatgccgggaatggacgggctggcgctgctcaagcagattaaacagcgccatccaatgcttccggtcatcattatgaccgcacattccgatctggatgctgccgtcagcgcctatcaacaaggggcgtttgattatctgcccaaaccgtttgatatcgacgaagcagtggcgctggttgagcgcgctatcagtcattaccaggaacagcagcagccgcgtaatgttcagcttaacggcccaacgaccgatatcatcggcgaagcgccagccatgcaggacgtgttccgtattatcggtcggctttcgcgttcttctattagcgtgctgattaacggcgaatccggcaccggtaaagaactggtcgctcatgccctgcatcgccacagtccgcgcgccaaagcgccgtttatcgcgctgaatatggcagctatcccaaaagatttgatcgaatcagaactgtttggccacgagaaaggcgcgtttactggcgcgaataccattcgtcaggggcgttttgaacaggccgatggcggtacattattcctcgacgaaattggtgatatgccgctggatgtgcagacgcgtttgctgcgcgtgctggcagacggtcagttttaccgcgttggcggctatgcgccggtgaaagtggatgtgcggattatcgctgccactcaccagaatctcgaacagcgagtgcaggaaggtaagttccgtgaggatctgttccaccgcctgaacgttatccgcgttcatctgccgccgctgcgcgaacgtcgggaagatattccccgtctggcgcgccattttttacaggttgccgcgcgcgaactgggcgtagaagcgaagttactgcatccggaaaccgaagctgctctgacgcgtctggcgtggccaggcaacgtgcgccagctggaaaacacctgccgctggctaacggtgatggccgccgggcaggaagtgttgattcaggatttgcccggcgaactgtttgaatcaacggttgcggagagtacttcgcaaatgcaaccggacagctgggcgacgcttcttgcgcagtgggcagacagagcgctgcgttccggtcatcaaaatctgctttccgaagcgcagccagagctggagcggacgttactgacgaccgcgttgcgacatacgcaggggcataaacaggaagcggcgcggctactcggctggggccgcaacaccctgacgcgtaagttaaaagagctggggatggagtgattcacagcttgtgtgtaaagattgattattgagcgcaaattgctggtattttacgctttactgttccaggaggaaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066547_sequence/1
type: Sequence
persistent identity: BBa K2066547 sequence
display id: BBa_K2066547_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacaattaagcttggcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z