LOCUS       BBa_K2066115            2754 bp    DNA     linear       27-Sep-2024
DEFINITION  Synthetic Enhancer Project: 2x TetO Binding Cassette (55as) + 
            sfGFP on UNS
ACCESSION   BBa_K2066115
VERSION     BBa_K2066115.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     misc_feature    41..87
                     /label=BBa_K2066543
     misc_feature    88..175
                     /label=BBa_K2066532
     promoter        176..205
                     /label=BBa_K2066544
     misc_feature    206..227
                     /label=BBa_K2066545
     misc_feature    228..370
                     /label=BBa_K2066547
     CDS             372..1865
                     /label=BBa_K2066546
     misc_feature    1866..2585
                     /label=BBa_K2066509
     stem_loop       2597..2640
                     /label=stem_loop
     misc_feature    2586..2665
                     /label=BBa_B0010
     terminator      2586..2665
                     /label=BBa_B0010
     stem_loop       2681..2700
                     /label=T7 TE
     misc_feature    2707..2707
                     /label=stop
     polyA_site      2701..2714
                     /label=polya
     misc_feature    2674..2714
                     /label=BBa_B0012
     terminator      2674..2714
                     /label=BBa_B0012
     terminator      2586..2714
                     /label=BBa_B0015
     misc_feature    2715..2754
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc tcacaattgc accaacatgg
       61 tgcttaatgt ttccattgaa gcactatatt ggtgcaacat tgtcttcgac gctagcagcg
      121 cgcggagatt ctagcccctt ttcgaggaac gtccccatga taacgccttt taggggcaat
      181 ttaaaagttg gcacagattt cgctttatct tttttaattg tgagcgctca caattaagct
      241 tggcgaaggt ggcaccgggc ttggcttatc catcgctcgt aatttgattg atcagcattc
      301 aggcaaaatt gaatttacca gttggccagg gcataccgag ttctcggttt acctgcctat
      361 caggaaataa acgtttatgc aacgagggat agtctgggta gtcgatgacg atagttccat
      421 ccgttgggtg cttgaacgtg cgctcgctgg ggcaggttta acctgtacga cgtttgagaa
      481 cggcgcagaa gtgctggagg cgctggcgag caaaacgccg gatgtgctgc tttcagatat
      541 ccgtatgccg ggaatggacg ggctggcgct gctcaagcag attaaacagc gccatccaat
      601 gcttccggtc atcattatga ccgcacattc cgatctggat gctgccgtca gcgcctatca
      661 acaaggggcg tttgattatc tgcccaaacc gtttgatatc gacgaagcag tggcgctggt
      721 tgagcgcgct atcagtcatt accaggaaca gcagcagccg cgtaatgttc agcttaacgg
      781 cccaacgacc gatatcatcg gcgaagcgcc agccatgcag gacgtgttcc gtattatcgg
      841 tcggctttcg cgttcttcta ttagcgtgct gattaacggc gaatccggca ccggtaaaga
      901 actggtcgct catgccctgc atcgccacag tccgcgcgcc aaagcgccgt ttatcgcgct
      961 gaatatggca gctatcccaa aagatttgat cgaatcagaa ctgtttggcc acgagaaagg
     1021 cgcgtttact ggcgcgaata ccattcgtca ggggcgtttt gaacaggccg atggcggtac
     1081 attattcctc gacgaaattg gtgatatgcc gctggatgtg cagacgcgtt tgctgcgcgt
     1141 gctggcagac ggtcagtttt accgcgttgg cggctatgcg ccggtgaaag tggatgtgcg
     1201 gattatcgct gccactcacc agaatctcga acagcgagtg caggaaggta agttccgtga
     1261 ggatctgttc caccgcctga acgttatccg cgttcatctg ccgccgctgc gcgaacgtcg
     1321 ggaagatatt ccccgtctgg cgcgccattt tttacaggtt gccgcgcgcg aactgggcgt
     1381 agaagcgaag ttactgcatc cggaaaccga agctgctctg acgcgtctgg cgtggccagg
     1441 caacgtgcgc cagctggaaa acacctgccg ctggctaacg gtgatggccg ccgggcagga
     1501 agtgttgatt caggatttgc ccggcgaact gtttgaatca acggttgcgg agagtacttc
     1561 gcaaatgcaa ccggacagct gggcgacgct tcttgcgcag tgggcagaca gagcgctgcg
     1621 ttccggtcat caaaatctgc tttccgaagc gcagccagag ctggagcgga cgttactgac
     1681 gaccgcgttg cgacatacgc aggggcataa acaggaagcg gcgcggctac tcggctgggg
     1741 ccgcaacacc ctgacgcgta agttaaaaga gctggggatg gagtgattca cagcttgtgt
     1801 gtaaagattg attattgagc gcaaattgct ggtattttac gctttactgt tccaggagga
     1861 aaaaaatgcg taaaggcgaa gagctgttca ctggtgtcgt ccctattctg gtggaactgg
     1921 atggtgatgt caacggtcat aagttttccg tgcgtggcga gggtgaaggt gacgcaacta
     1981 atggtaaact gacgctgaag ttcatctgta ctactggtaa actgccggta ccttggccga
     2041 ctctggtaac gacgctgact tatggtgttc agtgctttgc tcgttatccg gaccatatga
     2101 agcagcatga cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt
     2161 cctttaagga tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc
     2221 tggtaaaccg cattgagctg aaaggcattg actttaaaga agacggcaat atcctgggcc
     2281 ataagctgga atacaatttt aacagccaca atgtgtacat taccgcagat aaacaaaaaa
     2341 atggcattaa agcgaatttc aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg
     2401 ctgatcacta ccagcaaaac actccaatcg gtgatggtcc tgttctgctg ccagacaatc
     2461 actatctgag cacgcaaagc gttctgtcta aagatccgaa cgagaaacgc gatcatatgg
     2521 ttctgctgga gttcgtaacc gcagcgggca tcacgcatgg tatggatgaa ctgtacaaat
     2581 gatgaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     2641 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     2701 tttctgcgtt tatagcactg aaggtcctca atcgcactgg aaacatcaag gtcg
//