LOCUS       BBa_K2066116            2815 bp    DNA     linear       28-Jun-2024
DEFINITION  Synthetic Enhancer Project: 3x TetO Binding Cassette (52s) + sfGFP 
            on UNS
ACCESSION   BBa_K2066116
VERSION     BBa_K2066116.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_K2066018
     misc_feature    41..87
                     /label=BBa_K2066543
     misc_feature    88..236
                     /label=BBa_K2066531
     promoter        237..266
                     /label=BBa_K2066544
     misc_feature    267..288
                     /label=BBa_K2066545
     misc_feature    289..431
                     /label=BBa_K2066547
     CDS             433..1926
                     /label=BBa_K2066546
     misc_feature    1927..2646
                     /label=BBa_K2066509
     stem_loop       2658..2701
                     /label=stem_loop
     misc_feature    2647..2726
                     /label=BBa_B0010
     terminator      2647..2726
                     /label=BBa_B0010
     stem_loop       2742..2761
                     /label=T7 TE
     misc_feature    2768..2768
                     /label=stop
     polyA_site      2762..2775
                     /label=polya
     misc_feature    2735..2775
                     /label=BBa_B0012
     terminator      2735..2775
                     /label=BBa_B0012
     terminator      2647..2775
                     /label=BBa_B0015
     misc_feature    2776..2815
                     /label=BBa_K2066019
ORIGIN
        1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc tcacaattgc accaacatgg
       61 tgcttaatgt ttccattgaa gcactatatt ggtgcaacat tgtcttcgac gctagctctc
      121 tatcactgat agggatgtcg gaggctcgcg gactctatca atgatagagt ctcgagtctg
      181 gccggatctc tatcactgat agggagctag cgtccccatg ataacgcctt ttaggggcaa
      241 tttaaaagtt ggcacagatt tcgctttatc ttttttaatt gtgagcgctc acaattaagc
      301 ttggcgaagg tggcaccggg cttggcttat ccatcgctcg taatttgatt gatcagcatt
      361 caggcaaaat tgaatttacc agttggccag ggcataccga gttctcggtt tacctgccta
      421 tcaggaaata aacgtttatg caacgaggga tagtctgggt agtcgatgac gatagttcca
      481 tccgttgggt gcttgaacgt gcgctcgctg gggcaggttt aacctgtacg acgtttgaga
      541 acggcgcaga agtgctggag gcgctggcga gcaaaacgcc ggatgtgctg ctttcagata
      601 tccgtatgcc gggaatggac gggctggcgc tgctcaagca gattaaacag cgccatccaa
      661 tgcttccggt catcattatg accgcacatt ccgatctgga tgctgccgtc agcgcctatc
      721 aacaaggggc gtttgattat ctgcccaaac cgtttgatat cgacgaagca gtggcgctgg
      781 ttgagcgcgc tatcagtcat taccaggaac agcagcagcc gcgtaatgtt cagcttaacg
      841 gcccaacgac cgatatcatc ggcgaagcgc cagccatgca ggacgtgttc cgtattatcg
      901 gtcggctttc gcgttcttct attagcgtgc tgattaacgg cgaatccggc accggtaaag
      961 aactggtcgc tcatgccctg catcgccaca gtccgcgcgc caaagcgccg tttatcgcgc
     1021 tgaatatggc agctatccca aaagatttga tcgaatcaga actgtttggc cacgagaaag
     1081 gcgcgtttac tggcgcgaat accattcgtc aggggcgttt tgaacaggcc gatggcggta
     1141 cattattcct cgacgaaatt ggtgatatgc cgctggatgt gcagacgcgt ttgctgcgcg
     1201 tgctggcaga cggtcagttt taccgcgttg gcggctatgc gccggtgaaa gtggatgtgc
     1261 ggattatcgc tgccactcac cagaatctcg aacagcgagt gcaggaaggt aagttccgtg
     1321 aggatctgtt ccaccgcctg aacgttatcc gcgttcatct gccgccgctg cgcgaacgtc
     1381 gggaagatat tccccgtctg gcgcgccatt ttttacaggt tgccgcgcgc gaactgggcg
     1441 tagaagcgaa gttactgcat ccggaaaccg aagctgctct gacgcgtctg gcgtggccag
     1501 gcaacgtgcg ccagctggaa aacacctgcc gctggctaac ggtgatggcc gccgggcagg
     1561 aagtgttgat tcaggatttg cccggcgaac tgtttgaatc aacggttgcg gagagtactt
     1621 cgcaaatgca accggacagc tgggcgacgc ttcttgcgca gtgggcagac agagcgctgc
     1681 gttccggtca tcaaaatctg ctttccgaag cgcagccaga gctggagcgg acgttactga
     1741 cgaccgcgtt gcgacatacg caggggcata aacaggaagc ggcgcggcta ctcggctggg
     1801 gccgcaacac cctgacgcgt aagttaaaag agctggggat ggagtgattc acagcttgtg
     1861 tgtaaagatt gattattgag cgcaaattgc tggtatttta cgctttactg ttccaggagg
     1921 aaaaaaatgc gtaaaggcga agagctgttc actggtgtcg tccctattct ggtggaactg
     1981 gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg tgacgcaact
     2041 aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt accttggccg
     2101 actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg
     2161 aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga acgcacgatt
     2221 tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc
     2281 ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa tatcctgggc
     2341 cataagctgg aatacaattt taacagccac aatgtgtaca ttaccgcaga taaacaaaaa
     2401 aatggcatta aagcgaattt caaaattcgc cacaacgtgg aggatggcag cgtgcagctg
     2461 gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct gccagacaat
     2521 cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg cgatcatatg
     2581 gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga actgtacaaa
     2641 tgatgaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     2701 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2761 ctttctgcgt ttatagcact gaaggtcctc aatcgcactg gaaacatcaa ggtcg
//