BBa_K2066539 1 BBa_K2066539 UNS 3.1 2016-10-13T11:00:00Z 2016-10-19T02:36:08Z temp temp false false _2534_ 31541 27645 9 false tme false Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, &#65532;Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss BBa_K2066126 1 BBa_K2066126 Synthetic Enhancer Project: NRII2302 Upstream Regulatory Region with Promoter 2016-10-13T11:00:00Z 2016-10-19T05:55:12Z temp temp false false _2534_ 31541 27645 9 false temp false Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, &#65532;Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss annotation2500695 1 BBa_K2066018 range2500695 1 1 40 BBa_K2066543 1 BBa_K2066543 Synthetic Enhancer Project: Enhancer/NRI binding sites 2016-10-13T11:00:00Z 2016-10-19T04:20:03Z mep tmept false false _2534_ 31541 27645 9 false temp false Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, &#65532;Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K2066546 1 BBa_K2066546 Synthetic Enhancer Project: glnG (NRI) 2016-10-13T11:00:00Z 2016-10-19T05:07:26Z mept tempt false false _2534_ 31541 27645 9 false metp false Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, &#65532;Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss BBa_K2066547 1 BBa_K2066547 Synthetic Enhancer Project: Spacer between Promoter and RBS 2016-10-13T11:00:00Z 2016-10-19T05:11:55Z petm ptem false false _2534_ 31541 27645 9 false ep false Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, &#65532;Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K2066518 1 BBa_K2066518 mCherry (with start codon) 2016-10-07T11:00:00Z 2016-10-08T10:50:28Z Modified from the mCherry used in K2066028 and K2066029 to include the start codon. This modified mCherry is used in the construction of K2066040 and K2066041. false false _2534_ 31544 31544 9 false The start codon was removed in K2066028 and K2066029 to follow the design from Synthetic Analog Computation in Living Cells (Daniel et al.) where the codon bias was being optimized. false Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss BBa_K2066538 1 BBa_K2066538 Synthetic Enhancer Project: NRII2302 Mutant Coding 2016-10-13T11:00:00Z 2016-10-19T05:51:49Z te temp false false _2534_ 31541 27645 9 false mptem false Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, &#65532;Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss BBa_K2066540 1 BBa_K2066540 UNS 3.2 2016-10-13T11:00:00Z 2016-10-19T06:23:08Z tem temp false false _2534_ 31544 27645 9 false p false Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss BBa_K2066018 1 BBa_K2066018 UNS 2 Sequence, from Torella et al., 2013 2016-07-11T11:00:00Z 2016-10-19T05:41:43Z Torella, J. P., Boehm, C. R., Lienert, F., Chen, J. H., Way, J. C., & Silver, P. A. (2013). Rapid construction of insulated genetic circuits via synthetic sequence-guided isothermal assembly. Nucleic acids research, gkt860. This is Unique Nucleotide Sequence 2, (UNS 2), from Torella et al., 2013. The William and Mary iGEM team has adopted this as our standard prefix; as such, all of our parts will have this sequence immediately following the BioBrick prefix. We took this measure in order to allow easier Gibson Assembly cloning of our parts. Primer sequences which can be used to clone with the UNS 2/3 standard can be found on our wiki. false false _2534_ 31544 27446 9 false UNS 2 was chosen because it works well with UNS 3 and it is in accordance with the BioBrick standard. false Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss BBa_K2066117 1 BBa_K2066117 Synthetic Enhancer: 2x TetO Binding Cassette + NRII on UNS 2016-10-13T11:00:00Z 2016-10-28T09:57:29Z To be completed To be completed false false _2534_ 31541 31547 9 false To be completed false Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, &#65532;Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss component2523539 1 BBa_K2066532 component2523562 1 BBa_B0015 component2523552 1 BBa_K2066539 component2523540 1 BBa_K2066544 component2523555 1 BBa_K2066538 component2523543 1 BBa_K2066546 component2523563 1 BBa_K2066540 component2523554 1 BBa_K2066126 component2523542 1 BBa_K2066547 component2523538 1 BBa_K2066543 component2523537 1 BBa_K2066018 component2523544 1 BBa_K2066518 component2523541 1 BBa_K2066545 component2523551 1 BBa_B0015 component2523553 1 BBa_K2066018 annotation2523553 1 BBa_K2066018 range2523553 1 2729 2768 annotation2523543 1 BBa_K2066546 range2523543 1 372 1865 annotation2523537 1 BBa_K2066018 range2523537 1 1 40 annotation2523542 1 BBa_K2066547 range2523542 1 228 370 annotation2523562 1 BBa_B0015 range2523562 1 3846 3974 annotation2523540 1 BBa_K2066544 range2523540 1 176 205 annotation2523539 1 BBa_K2066532 range2523539 1 88 175 annotation2523541 1 BBa_K2066545 range2523541 1 206 227 annotation2523544 1 BBa_K2066518 range2523544 1 1866 2579 annotation2523551 1 BBa_B0015 range2523551 1 2580 2708 annotation2523552 1 BBa_K2066539 range2523552 1 2709 2728 annotation2523554 1 BBa_K2066126 range2523554 1 2729 2795 annotation2523555 1 BBa_K2066538 range2523555 1 2796 3845 annotation2523538 1 BBa_K2066543 range2523538 1 41 87 annotation2523563 1 BBa_K2066540 range2523563 1 3975 3994 BBa_K2066544 1 BBa_K2066544 Synthetic Enhancer Project: glnAp2 promoter 2016-10-13T11:00:00Z 2016-10-19T04:48:44Z temp temp false false _2534_ 31541 27645 9 false temp false Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, &#65532;Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K2066532 1 BBa_K2066532 Synthetic Enhancer Project: 2X TetO Binding Cassette (55AS) 2016-10-13T11:00:00Z 2016-10-19T05:15:03Z . / false false _2534_ 31541 27645 9 false . false Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, &#65532;Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss BBa_K2066545 1 BBa_K2066545 Synthetic Enhancer Project: LacI binding site (LacO) 2016-10-13T11:00:00Z 2016-10-19T05:05:36Z temp temp false false _2534_ 31541 27645 9 false tem false Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss BBa_K2066532_sequence 1 attggtgcaacattgtcttcgacgctagcagcgcgcggagattctagccccttttcgaggaacgtccccatgataacgccttttaggg BBa_K2066539_sequence 1 gcactgaaggtcctcaatcg BBa_K2066543_sequence 1 tcacaattgcaccaacatggtgcttaatgtttccattgaagcactat BBa_K2066545_sequence 1 tatcttttttaattgtgagcgc BBa_K2066540_sequence 1 cactggaaacatcaaggtcg BBa_K2066544_sequence 1 gcaatttaaaagttggcacagatttcgctt BBa_K2066018_sequence 1 gctgggagttcgtagacggaaacaaacgcagaatccaagc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K2066518_sequence 1 atggtgagcaagggcgaagaagataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa BBa_K2066126_sequence 1 ggtaggccggagcaggtgagtcgctctccaacgtgaagtttgtcagctatctgtagcccatctctgc BBa_K2066546_sequence 1 cgtttatgcaacgagggatagtctgggtagtcgatgacgatagttccatccgttgggtgcttgaacgtgcgctcgctggggcaggtttaacctgtacgacgtttgagaacggcgcagaagtgctggaggcgctggcgagcaaaacgccggatgtgctgctttcagatatccgtatgccgggaatggacgggctggcgctgctcaagcagattaaacagcgccatccaatgcttccggtcatcattatgaccgcacattccgatctggatgctgccgtcagcgcctatcaacaaggggcgtttgattatctgcccaaaccgtttgatatcgacgaagcagtggcgctggttgagcgcgctatcagtcattaccaggaacagcagcagccgcgtaatgttcagcttaacggcccaacgaccgatatcatcggcgaagcgccagccatgcaggacgtgttccgtattatcggtcggctttcgcgttcttctattagcgtgctgattaacggcgaatccggcaccggtaaagaactggtcgctcatgccctgcatcgccacagtccgcgcgccaaagcgccgtttatcgcgctgaatatggcagctatcccaaaagatttgatcgaatcagaactgtttggccacgagaaaggcgcgtttactggcgcgaataccattcgtcaggggcgttttgaacaggccgatggcggtacattattcctcgacgaaattggtgatatgccgctggatgtgcagacgcgtttgctgcgcgtgctggcagacggtcagttttaccgcgttggcggctatgcgccggtgaaagtggatgtgcggattatcgctgccactcaccagaatctcgaacagcgagtgcaggaaggtaagttccgtgaggatctgttccaccgcctgaacgttatccgcgttcatctgccgccgctgcgcgaacgtcgggaagatattccccgtctggcgcgccattttttacaggttgccgcgcgcgaactgggcgtagaagcgaagttactgcatccggaaaccgaagctgctctgacgcgtctggcgtggccaggcaacgtgcgccagctggaaaacacctgccgctggctaacggtgatggccgccgggcaggaagtgttgattcaggatttgcccggcgaactgtttgaatcaacggttgcggagagtacttcgcaaatgcaaccggacagctgggcgacgcttcttgcgcagtgggcagacagagcgctgcgttccggtcatcaaaatctgctttccgaagcgcagccagagctggagcggacgttactgacgaccgcgttgcgacatacgcaggggcataaacaggaagcggcgcggctactcggctggggccgcaacaccctgacgcgtaagttaaaagagctggggatggagtgattcacagcttgtgtgtaaagattgattattgagcgcaaattgctggtattttacgctttactgttccaggaggaaaaaa BBa_K2066538_sequence 1 atggcaacaggcacgcagcccgatgctgggcagatcctcaactcgctgattaacagtattttgttaatcgatgacaacctggcgatccattacgccaaccctgccgcgcaacaactgctcgcccaaagctcccgcaaattgtttggtacaccgttaccggaactgttgagctacttctcattaaatatcgagctgatgcaagaaagtctggaggcggggcaaggttttaccgataacgaagtgacgctggtcatcgacgggcgctcgcatatcctttctgtgacggcccagcgtatgccggacggcatgatcctgctggagatggctccgatggataaccagcgccgcttaagtcaggaacagctacagcacgcccagcaggttactgcccgtgatttagtgcgcggcctggcacatgagattaaaaatccgcttggcggtttacgtggcgcggcgcagctgctcagcaaagcgttacctgacccatcactactcgaatataccaaagtgattatcgaacaggcggaccggctgcgaaatctggtcgaccgtctgttggggccgcagctgcccggtacgcgcgttaccgaaagtattcacaaagtggctgaacgcgtggtaacgctggtgtcgatggaactgccggacaacgtgcggttgattcgtgattacgatcccagcctaccggaactggcgcacgacccggatcaaattgaacaggtcttgctgaatattgtgcgcaatgcgctacaggcgctggggccggaaggcggtgaaatcattctgcgtacccgcaccgcgtttcaactgaccttacacggcgagcgctaccggctggcggcgcggattgatgtggaagataacgggccgggcattccgcctcatttgcaggatacgctgttttacccgatggtcagcggccgcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2066117_sequence 1 gctgggagttcgtagacggaaacaaacgcagaatccaagctcacaattgcaccaacatggtgcttaatgtttccattgaagcactatattggtgcaacattgtcttcgacgctagcagcgcgcggagattctagccccttttcgaggaacgtccccatgataacgccttttaggggcaatttaaaagttggcacagatttcgctttatcttttttaattgtgagcgctcacaattaagcttggcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataaacgtttatgcaacgagggatagtctgggtagtcgatgacgatagttccatccgttgggtgcttgaacgtgcgctcgctggggcaggtttaacctgtacgacgtttgagaacggcgcagaagtgctggaggcgctggcgagcaaaacgccggatgtgctgctttcagatatccgtatgccgggaatggacgggctggcgctgctcaagcagattaaacagcgccatccaatgcttccggtcatcattatgaccgcacattccgatctggatgctgccgtcagcgcctatcaacaaggggcgtttgattatctgcccaaaccgtttgatatcgacgaagcagtggcgctggttgagcgcgctatcagtcattaccaggaacagcagcagccgcgtaatgttcagcttaacggcccaacgaccgatatcatcggcgaagcgccagccatgcaggacgtgttccgtattatcggtcggctttcgcgttcttctattagcgtgctgattaacggcgaatccggcaccggtaaagaactggtcgctcatgccctgcatcgccacagtccgcgcgccaaagcgccgtttatcgcgctgaatatggcagctatcccaaaagatttgatcgaatcagaactgtttggccacgagaaaggcgcgtttactggcgcgaataccattcgtcaggggcgttttgaacaggccgatggcggtacattattcctcgacgaaattggtgatatgccgctggatgtgcagacgcgtttgctgcgcgtgctggcagacggtcagttttaccgcgttggcggctatgcgccggtgaaagtggatgtgcggattatcgctgccactcaccagaatctcgaacagcgagtgcaggaaggtaagttccgtgaggatctgttccaccgcctgaacgttatccgcgttcatctgccgccgctgcgcgaacgtcgggaagatattccccgtctggcgcgccattttttacaggttgccgcgcgcgaactgggcgtagaagcgaagttactgcatccggaaaccgaagctgctctgacgcgtctggcgtggccaggcaacgtgcgccagctggaaaacacctgccgctggctaacggtgatggccgccgggcaggaagtgttgattcaggatttgcccggcgaactgtttgaatcaacggttgcggagagtacttcgcaaatgcaaccggacagctgggcgacgcttcttgcgcagtgggcagacagagcgctgcgttccggtcatcaaaatctgctttccgaagcgcagccagagctggagcggacgttactgacgaccgcgttgcgacatacgcaggggcataaacaggaagcggcgcggctactcggctggggccgcaacaccctgacgcgtaagttaaaagagctggggatggagtgattcacagcttgtgtgtaaagattgattattgagcgcaaattgctggtattttacgctttactgttccaggaggaaaaaaatggtgagcaagggcgaagaagataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagcactgaaggtcctcaatcgggtaggccggagcaggtgagtcgctctccaacgtgaagtttgtcagctatctgtagcccatctctgcatggcaacaggcacgcagcccgatgctgggcagatcctcaactcgctgattaacagtattttgttaatcgatgacaacctggcgatccattacgccaaccctgccgcgcaacaactgctcgcccaaagctcccgcaaattgtttggtacaccgttaccggaactgttgagctacttctcattaaatatcgagctgatgcaagaaagtctggaggcggggcaaggttttaccgataacgaagtgacgctggtcatcgacgggcgctcgcatatcctttctgtgacggcccagcgtatgccggacggcatgatcctgctggagatggctccgatggataaccagcgccgcttaagtcaggaacagctacagcacgcccagcaggttactgcccgtgatttagtgcgcggcctggcacatgagattaaaaatccgcttggcggtttacgtggcgcggcgcagctgctcagcaaagcgttacctgacccatcactactcgaatataccaaagtgattatcgaacaggcggaccggctgcgaaatctggtcgaccgtctgttggggccgcagctgcccggtacgcgcgttaccgaaagtattcacaaagtggctgaacgcgtggtaacgctggtgtcgatggaactgccggacaacgtgcggttgattcgtgattacgatcccagcctaccggaactggcgcacgacccggatcaaattgaacaggtcttgctgaatattgtgcgcaatgcgctacaggcgctggggccggaaggcggtgaaatcattctgcgtacccgcaccgcgtttcaactgaccttacacggcgagcgctaccggctggcggcgcggattgatgtggaagataacgggccgggcattccgcctcatttgcaggatacgctgttttacccgatggtcagcggccgcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacactggaaacatcaaggtcg BBa_K2066547_sequence 1 tcacaattaagcttggcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataa BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z