LOCUS BBa_K2066119 4000 bp DNA linear 22-Nov-2024 DEFINITION Synthetic Enhancer Project: 2X TetO Binding Cassette(52S) + NRII + sfGFP on UNS ACCESSION BBa_K2066119 VERSION BBa_K2066119.1 FEATURES Location/Qualifiers misc_feature 1..40 /label=BBa_K2066018 misc_feature 41..87 /label=BBa_K2066543 misc_feature 88..175 /label=BBa_K2066532 promoter 176..205 /label=BBa_K2066544 misc_feature 206..227 /label=BBa_K2066545 misc_feature 228..370 /label=BBa_K2066547 CDS 372..1865 /label=BBa_K2066546 misc_feature 1866..2585 /label=BBa_K2066509 stem_loop 2597..2640 /label=stem_loop misc_feature 2586..2665 /label=BBa_B0010 terminator 2586..2665 /label=BBa_B0010 stem_loop 2681..2700 /label=T7 TE misc_feature 2707..2707 /label=stop polyA_site 2701..2714 /label=polya misc_feature 2674..2714 /label=BBa_B0012 terminator 2674..2714 /label=BBa_B0012 terminator 2586..2714 /label=BBa_B0015 misc_feature 2715..2734 /label=BBa_K2066539 misc_feature 2735..2774 /label=BBa_K2066018 misc_feature 2735..2774 /label=BBa_K2066018 promoter 2735..2801 /label=BBa_K2066126 CDS 2802..3851 /label=BBa_K2066538 stem_loop 3863..3906 /label=stem_loop misc_feature 3852..3931 /label=BBa_B0010 terminator 3852..3931 /label=BBa_B0010 stem_loop 3947..3966 /label=T7 TE misc_feature 3973..3973 /label=stop polyA_site 3967..3980 /label=polya misc_feature 3940..3980 /label=BBa_B0012 terminator 3940..3980 /label=BBa_B0012 terminator 3852..3980 /label=BBa_B0015 misc_feature 3981..4000 /label=BBa_K2066540 ORIGIN 1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc tcacaattgc accaacatgg 61 tgcttaatgt ttccattgaa gcactatatt ggtgcaacat tgtcttcgac gctagcagcg 121 cgcggagatt ctagcccctt ttcgaggaac gtccccatga taacgccttt taggggcaat 181 ttaaaagttg gcacagattt cgctttatct tttttaattg tgagcgctca caattaagct 241 tggcgaaggt ggcaccgggc ttggcttatc catcgctcgt aatttgattg atcagcattc 301 aggcaaaatt gaatttacca gttggccagg gcataccgag ttctcggttt acctgcctat 361 caggaaataa acgtttatgc aacgagggat agtctgggta gtcgatgacg atagttccat 421 ccgttgggtg cttgaacgtg cgctcgctgg ggcaggttta acctgtacga cgtttgagaa 481 cggcgcagaa gtgctggagg cgctggcgag caaaacgccg gatgtgctgc tttcagatat 541 ccgtatgccg ggaatggacg ggctggcgct gctcaagcag attaaacagc gccatccaat 601 gcttccggtc atcattatga ccgcacattc cgatctggat gctgccgtca gcgcctatca 661 acaaggggcg tttgattatc tgcccaaacc gtttgatatc gacgaagcag tggcgctggt 721 tgagcgcgct atcagtcatt accaggaaca gcagcagccg cgtaatgttc agcttaacgg 781 cccaacgacc gatatcatcg gcgaagcgcc agccatgcag gacgtgttcc gtattatcgg 841 tcggctttcg cgttcttcta ttagcgtgct gattaacggc gaatccggca ccggtaaaga 901 actggtcgct catgccctgc atcgccacag tccgcgcgcc aaagcgccgt ttatcgcgct 961 gaatatggca gctatcccaa aagatttgat cgaatcagaa ctgtttggcc acgagaaagg 1021 cgcgtttact ggcgcgaata ccattcgtca ggggcgtttt gaacaggccg atggcggtac 1081 attattcctc gacgaaattg gtgatatgcc gctggatgtg cagacgcgtt tgctgcgcgt 1141 gctggcagac ggtcagtttt accgcgttgg cggctatgcg ccggtgaaag tggatgtgcg 1201 gattatcgct gccactcacc agaatctcga acagcgagtg caggaaggta agttccgtga 1261 ggatctgttc caccgcctga acgttatccg cgttcatctg ccgccgctgc gcgaacgtcg 1321 ggaagatatt ccccgtctgg cgcgccattt tttacaggtt gccgcgcgcg aactgggcgt 1381 agaagcgaag ttactgcatc cggaaaccga agctgctctg acgcgtctgg cgtggccagg 1441 caacgtgcgc cagctggaaa acacctgccg ctggctaacg gtgatggccg ccgggcagga 1501 agtgttgatt caggatttgc ccggcgaact gtttgaatca acggttgcgg agagtacttc 1561 gcaaatgcaa ccggacagct gggcgacgct tcttgcgcag tgggcagaca gagcgctgcg 1621 ttccggtcat caaaatctgc tttccgaagc gcagccagag ctggagcgga cgttactgac 1681 gaccgcgttg cgacatacgc aggggcataa acaggaagcg gcgcggctac tcggctgggg 1741 ccgcaacacc ctgacgcgta agttaaaaga gctggggatg gagtgattca cagcttgtgt 1801 gtaaagattg attattgagc gcaaattgct ggtattttac gctttactgt tccaggagga 1861 aaaaaatgcg taaaggcgaa gagctgttca ctggtgtcgt ccctattctg gtggaactgg 1921 atggtgatgt caacggtcat aagttttccg tgcgtggcga gggtgaaggt gacgcaacta 1981 atggtaaact gacgctgaag ttcatctgta ctactggtaa actgccggta ccttggccga 2041 ctctggtaac gacgctgact tatggtgttc agtgctttgc tcgttatccg gaccatatga 2101 agcagcatga cttcttcaag tccgccatgc cggaaggcta tgtgcaggaa cgcacgattt 2161 cctttaagga tgacggcacg tacaaaacgc gtgcggaagt gaaatttgaa ggcgataccc 2221 tggtaaaccg cattgagctg aaaggcattg actttaaaga agacggcaat atcctgggcc 2281 ataagctgga atacaatttt aacagccaca atgtgtacat taccgcagat aaacaaaaaa 2341 atggcattaa agcgaatttc aaaattcgcc acaacgtgga ggatggcagc gtgcagctgg 2401 ctgatcacta ccagcaaaac actccaatcg gtgatggtcc tgttctgctg ccagacaatc 2461 actatctgag cacgcaaagc gttctgtcta aagatccgaa cgagaaacgc gatcatatgg 2521 ttctgctgga gttcgtaacc gcagcgggca tcacgcatgg tatggatgaa ctgtacaaat 2581 gatgaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 2641 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc 2701 tttctgcgtt tatagcactg aaggtcctca atcgggtagg ccggagcagg tgagtcgctc 2761 tccaacgtga agtttgtcag ctatctgtag cccatctctg catggcaaca ggcacgcagc 2821 ccgatgctgg gcagatcctc aactcgctga ttaacagtat tttgttaatc gatgacaacc 2881 tggcgatcca ttacgccaac cctgccgcgc aacaactgct cgcccaaagc tcccgcaaat 2941 tgtttggtac accgttaccg gaactgttga gctacttctc attaaatatc gagctgatgc 3001 aagaaagtct ggaggcgggg caaggtttta ccgataacga agtgacgctg gtcatcgacg 3061 ggcgctcgca tatcctttct gtgacggccc agcgtatgcc ggacggcatg atcctgctgg 3121 agatggctcc gatggataac cagcgccgct taagtcagga acagctacag cacgcccagc 3181 aggttactgc ccgtgattta gtgcgcggcc tggcacatga gattaaaaat ccgcttggcg 3241 gtttacgtgg cgcggcgcag ctgctcagca aagcgttacc tgacccatca ctactcgaat 3301 ataccaaagt gattatcgaa caggcggacc ggctgcgaaa tctggtcgac cgtctgttgg 3361 ggccgcagct gcccggtacg cgcgttaccg aaagtattca caaagtggct gaacgcgtgg 3421 taacgctggt gtcgatggaa ctgccggaca acgtgcggtt gattcgtgat tacgatccca 3481 gcctaccgga actggcgcac gacccggatc aaattgaaca ggtcttgctg aatattgtgc 3541 gcaatgcgct acaggcgctg gggccggaag gcggtgaaat cattctgcgt acccgcaccg 3601 cgtttcaact gaccttacac ggcgagcgct accggctggc ggcgcggatt gatgtggaag 3661 ataacgggcc gggcattccg cctcatttgc aggatacgct gttttacccg atggtcagcg 3721 gccgcgaagg tggcaccggg cttggcttat ccatcgctcg taatttgatt gatcagcatt 3781 caggcaaaat tgaatttacc agttggccag ggcataccga gttctcggtt tacctgccta 3841 tcaggaaata accaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 3901 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 3961 tgggcctttc tgcgtttata cactggaaac atcaaggtcg //