LOCUS BBa_K2066120 4061 bp DNA linear 27-Dec-2024 DEFINITION Synthetic Enhancer Project: 3X TetO Binding Cassette(52S) + NRII + sfGFP on UNS ACCESSION BBa_K2066120 VERSION BBa_K2066120.1 FEATURES Location/Qualifiers misc_feature 1..40 /label=BBa_K2066018 misc_feature 41..87 /label=BBa_K2066543 misc_feature 88..236 /label=BBa_K2066531 promoter 237..266 /label=BBa_K2066544 misc_feature 267..288 /label=BBa_K2066545 misc_feature 289..431 /label=BBa_K2066547 CDS 433..1926 /label=BBa_K2066546 misc_feature 1927..2646 /label=BBa_K2066509 stem_loop 2658..2701 /label=stem_loop misc_feature 2647..2726 /label=BBa_B0010 terminator 2647..2726 /label=BBa_B0010 stem_loop 2742..2761 /label=T7 TE misc_feature 2768..2768 /label=stop polyA_site 2762..2775 /label=polya misc_feature 2735..2775 /label=BBa_B0012 terminator 2735..2775 /label=BBa_B0012 terminator 2647..2775 /label=BBa_B0015 misc_feature 2776..2795 /label=BBa_K2066539 misc_feature 2796..2835 /label=BBa_K2066018 misc_feature 2796..2835 /label=BBa_K2066018 promoter 2796..2862 /label=BBa_K2066126 CDS 2863..3912 /label=BBa_K2066538 stem_loop 3924..3967 /label=stem_loop misc_feature 3913..3992 /label=BBa_B0010 terminator 3913..3992 /label=BBa_B0010 stem_loop 4008..4027 /label=T7 TE misc_feature 4034..4034 /label=stop polyA_site 4028..4041 /label=polya misc_feature 4001..4041 /label=BBa_B0012 terminator 4001..4041 /label=BBa_B0012 terminator 3913..4041 /label=BBa_B0015 misc_feature 4042..4061 /label=BBa_K2066540 ORIGIN 1 gctgggagtt cgtagacgga aacaaacgca gaatccaagc tcacaattgc accaacatgg 61 tgcttaatgt ttccattgaa gcactatatt ggtgcaacat tgtcttcgac gctagctctc 121 tatcactgat agggatgtcg gaggctcgcg gactctatca atgatagagt ctcgagtctg 181 gccggatctc tatcactgat agggagctag cgtccccatg ataacgcctt ttaggggcaa 241 tttaaaagtt ggcacagatt tcgctttatc ttttttaatt gtgagcgctc acaattaagc 301 ttggcgaagg tggcaccggg cttggcttat ccatcgctcg taatttgatt gatcagcatt 361 caggcaaaat tgaatttacc agttggccag ggcataccga gttctcggtt tacctgccta 421 tcaggaaata aacgtttatg caacgaggga tagtctgggt agtcgatgac gatagttcca 481 tccgttgggt gcttgaacgt gcgctcgctg gggcaggttt aacctgtacg acgtttgaga 541 acggcgcaga agtgctggag gcgctggcga gcaaaacgcc ggatgtgctg ctttcagata 601 tccgtatgcc gggaatggac gggctggcgc tgctcaagca gattaaacag cgccatccaa 661 tgcttccggt catcattatg accgcacatt ccgatctgga tgctgccgtc agcgcctatc 721 aacaaggggc gtttgattat ctgcccaaac cgtttgatat cgacgaagca gtggcgctgg 781 ttgagcgcgc tatcagtcat taccaggaac agcagcagcc gcgtaatgtt cagcttaacg 841 gcccaacgac cgatatcatc ggcgaagcgc cagccatgca ggacgtgttc cgtattatcg 901 gtcggctttc gcgttcttct attagcgtgc tgattaacgg cgaatccggc accggtaaag 961 aactggtcgc tcatgccctg catcgccaca gtccgcgcgc caaagcgccg tttatcgcgc 1021 tgaatatggc agctatccca aaagatttga tcgaatcaga actgtttggc cacgagaaag 1081 gcgcgtttac tggcgcgaat accattcgtc aggggcgttt tgaacaggcc gatggcggta 1141 cattattcct cgacgaaatt ggtgatatgc cgctggatgt gcagacgcgt ttgctgcgcg 1201 tgctggcaga cggtcagttt taccgcgttg gcggctatgc gccggtgaaa gtggatgtgc 1261 ggattatcgc tgccactcac cagaatctcg aacagcgagt gcaggaaggt aagttccgtg 1321 aggatctgtt ccaccgcctg aacgttatcc gcgttcatct gccgccgctg cgcgaacgtc 1381 gggaagatat tccccgtctg gcgcgccatt ttttacaggt tgccgcgcgc gaactgggcg 1441 tagaagcgaa gttactgcat ccggaaaccg aagctgctct gacgcgtctg gcgtggccag 1501 gcaacgtgcg ccagctggaa aacacctgcc gctggctaac ggtgatggcc gccgggcagg 1561 aagtgttgat tcaggatttg cccggcgaac tgtttgaatc aacggttgcg gagagtactt 1621 cgcaaatgca accggacagc tgggcgacgc ttcttgcgca gtgggcagac agagcgctgc 1681 gttccggtca tcaaaatctg ctttccgaag cgcagccaga gctggagcgg acgttactga 1741 cgaccgcgtt gcgacatacg caggggcata aacaggaagc ggcgcggcta ctcggctggg 1801 gccgcaacac cctgacgcgt aagttaaaag agctggggat ggagtgattc acagcttgtg 1861 tgtaaagatt gattattgag cgcaaattgc tggtatttta cgctttactg ttccaggagg 1921 aaaaaaatgc gtaaaggcga agagctgttc actggtgtcg tccctattct ggtggaactg 1981 gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg tgacgcaact 2041 aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt accttggccg 2101 actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg 2161 aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga acgcacgatt 2221 tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc 2281 ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa tatcctgggc 2341 cataagctgg aatacaattt taacagccac aatgtgtaca ttaccgcaga taaacaaaaa 2401 aatggcatta aagcgaattt caaaattcgc cacaacgtgg aggatggcag cgtgcagctg 2461 gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct gccagacaat 2521 cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg cgatcatatg 2581 gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga actgtacaaa 2641 tgatgaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta 2701 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc 2761 ctttctgcgt ttatagcact gaaggtcctc aatcgggtag gccggagcag gtgagtcgct 2821 ctccaacgtg aagtttgtca gctatctgta gcccatctct gcatggcaac aggcacgcag 2881 cccgatgctg ggcagatcct caactcgctg attaacagta ttttgttaat cgatgacaac 2941 ctggcgatcc attacgccaa ccctgccgcg caacaactgc tcgcccaaag ctcccgcaaa 3001 ttgtttggta caccgttacc ggaactgttg agctacttct cattaaatat cgagctgatg 3061 caagaaagtc tggaggcggg gcaaggtttt accgataacg aagtgacgct ggtcatcgac 3121 gggcgctcgc atatcctttc tgtgacggcc cagcgtatgc cggacggcat gatcctgctg 3181 gagatggctc cgatggataa ccagcgccgc ttaagtcagg aacagctaca gcacgcccag 3241 caggttactg cccgtgattt agtgcgcggc ctggcacatg agattaaaaa tccgcttggc 3301 ggtttacgtg gcgcggcgca gctgctcagc aaagcgttac ctgacccatc actactcgaa 3361 tataccaaag tgattatcga acaggcggac cggctgcgaa atctggtcga ccgtctgttg 3421 gggccgcagc tgcccggtac gcgcgttacc gaaagtattc acaaagtggc tgaacgcgtg 3481 gtaacgctgg tgtcgatgga actgccggac aacgtgcggt tgattcgtga ttacgatccc 3541 agcctaccgg aactggcgca cgacccggat caaattgaac aggtcttgct gaatattgtg 3601 cgcaatgcgc tacaggcgct ggggccggaa ggcggtgaaa tcattctgcg tacccgcacc 3661 gcgtttcaac tgaccttaca cggcgagcgc taccggctgg cggcgcggat tgatgtggaa 3721 gataacgggc cgggcattcc gcctcatttg caggatacgc tgttttaccc gatggtcagc 3781 ggccgcgaag gtggcaccgg gcttggctta tccatcgctc gtaatttgat tgatcagcat 3841 tcaggcaaaa ttgaatttac cagttggcca gggcataccg agttctcggt ttacctgcct 3901 atcaggaaat aaccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 3961 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 4021 gtgggccttt ctgcgtttat acactggaaa catcaaggtc g //