##gff-version 3
##sequence-region BBa_K2066121 1 4083
BBa_K2066121	.	sequence_feature	311	453	.	+	0	ID=BBa_K2066547;Name=BBa_K2066547
BBa_K2066121	.	promoter	2818	2884	.	+	0	ID=BBa_K2066126;Name=BBa_K2066126
BBa_K2066121	.	engineered_region	2818	2857	.	+	0	ID=annotation2500695;Name=BBa_K2066018;Parent=BBa_K2066126
BBa_K2066121	.	engineered_region	1949	2668	.	+	0	ID=BBa_K2066509;Name=BBa_K2066509
BBa_K2066121	.	sequence_feature	104	123	.	+	0	ID=BBa_K2066542;Name=BBa_K2066542
BBa_K2066121	.	terminator	3935	4063	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2066121	.	terminator	3935	4014	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2066121	.	engineered_region	3935	4014	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2066121	.	stem_loop	3946	3989	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2066121	.	terminator	4023	4063	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2066121	.	engineered_region	4023	4063	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2066121	.	stop_codon	4056	4056	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2066121	.	polyA_site	4050	4063	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2066121	.	stem_loop	4030	4049	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2066121	.	promoter	259	288	.	+	0	ID=BBa_K2066544;Name=BBa_K2066544
BBa_K2066121	.	CDS	2885	3934	.	+	0	ID=BBa_K2066538;Name=BBa_K2066538
BBa_K2066121	.	sequence_feature	289	310	.	+	0	ID=BBa_K2066545;Name=BBa_K2066545
BBa_K2066121	.	sequence_feature	1	20	.	+	0	ID=BBa_K2066541;Name=BBa_K2066541
BBa_K2066121	.	sequence_feature	4064	4083	.	+	0	ID=BBa_K2066540;Name=BBa_K2066540
BBa_K2066121	.	CDS	455	1948	.	+	0	ID=BBa_K2066546;Name=BBa_K2066546
BBa_K2066121	.	sequence_feature	2798	2817	.	+	0	ID=BBa_K2066539;Name=BBa_K2066539
BBa_K2066121	.	sequence_feature	171	258	.	+	0	ID=BBa_K2066532;Name=BBa_K2066532
BBa_K2066121	.	promoter	21	103	.	+	0	ID=BBa_I739101;Name=BBa_I739101
BBa_K2066121	.	operator	64	103	.	+	0	ID=annotation1946257;Name=R0040 (2x TetR 1);Parent=BBa_I739101
BBa_K2066121	.	non_covalent_binding_site	84	103	.	+	0	ID=annotation1946101;Name=TetR 1;Parent=BBa_I739101
BBa_K2066121	.	promoter	21	55	.	+	0	ID=annotation1946100;Name=J23100;Parent=BBa_I739101
BBa_K2066121	.	non_covalent_binding_site	64	83	.	+	0	ID=annotation1946102;Name=TetR 1;Parent=BBa_I739101
BBa_K2066121	.	sequence_feature	56	63	.	+	0	ID=annotation1946099;Name=Scar;Parent=BBa_I739101
BBa_K2066121	.	sequence_feature	124	170	.	+	0	ID=BBa_K2066543;Name=BBa_K2066543
BBa_K2066121	.	terminator	2669	2797	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2066121	.	terminator	2669	2748	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2066121	.	engineered_region	2669	2748	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2066121	.	stem_loop	2680	2723	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2066121	.	terminator	2757	2797	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2066121	.	engineered_region	2757	2797	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2066121	.	stop_codon	2790	2790	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2066121	.	polyA_site	2784	2797	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2066121	.	stem_loop	2764	2783	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
>BBa_K2066121
gctgggagttcgtagacggattgacggctagctcagtcctaggtacagtgctagctactagagtccctatcagtgatag
agattccctatcagtgatagagataacaaacgcagaatccaagctcacaattgcaccaacatggtgcttaatgtttcca
ttgaagcactatattggtgcaacattgtcttcgacgctagcagcgcgcggagattctagccccttttcgaggaacgtcc
ccatgataacgccttttaggggcaatttaaaagttggcacagatttcgctttatcttttttaattgtgagcgctcacaa
ttaagcttggcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattga
atttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataaacgtttatgcaacgagggata
gtctgggtagtcgatgacgatagttccatccgttgggtgcttgaacgtgcgctcgctggggcaggtttaacctgtacga
cgtttgagaacggcgcagaagtgctggaggcgctggcgagcaaaacgccggatgtgctgctttcagatatccgtatgcc
gggaatggacgggctggcgctgctcaagcagattaaacagcgccatccaatgcttccggtcatcattatgaccgcacat
tccgatctggatgctgccgtcagcgcctatcaacaaggggcgtttgattatctgcccaaaccgtttgatatcgacgaag
cagtggcgctggttgagcgcgctatcagtcattaccaggaacagcagcagccgcgtaatgttcagcttaacggcccaac
gaccgatatcatcggcgaagcgccagccatgcaggacgtgttccgtattatcggtcggctttcgcgttcttctattagc
gtgctgattaacggcgaatccggcaccggtaaagaactggtcgctcatgccctgcatcgccacagtccgcgcgccaaag
cgccgtttatcgcgctgaatatggcagctatcccaaaagatttgatcgaatcagaactgtttggccacgagaaaggcgc
gtttactggcgcgaataccattcgtcaggggcgttttgaacaggccgatggcggtacattattcctcgacgaaattggt
gatatgccgctggatgtgcagacgcgtttgctgcgcgtgctggcagacggtcagttttaccgcgttggcggctatgcgc
cggtgaaagtggatgtgcggattatcgctgccactcaccagaatctcgaacagcgagtgcaggaaggtaagttccgtga
ggatctgttccaccgcctgaacgttatccgcgttcatctgccgccgctgcgcgaacgtcgggaagatattccccgtctg
gcgcgccattttttacaggttgccgcgcgcgaactgggcgtagaagcgaagttactgcatccggaaaccgaagctgctc
tgacgcgtctggcgtggccaggcaacgtgcgccagctggaaaacacctgccgctggctaacggtgatggccgccgggca
ggaagtgttgattcaggatttgcccggcgaactgtttgaatcaacggttgcggagagtacttcgcaaatgcaaccggac
agctgggcgacgcttcttgcgcagtgggcagacagagcgctgcgttccggtcatcaaaatctgctttccgaagcgcagc
cagagctggagcggacgttactgacgaccgcgttgcgacatacgcaggggcataaacaggaagcggcgcggctactcgg
ctggggccgcaacaccctgacgcgtaagttaaaagagctggggatggagtgattcacagcttgtgtgtaaagattgatt
attgagcgcaaattgctggtattttacgctttactgttccaggaggaaaaaaatgcgtaaaggcgaagagctgttcact
ggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtg
acgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaac
gacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatg
ccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttg
aaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagct
ggaatacaattttaacagccacaatgtgtacattaccgcagataaacaaaaaaatggcattaaagcgaatttcaaaatt
cgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttc
tgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttct
gctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatgaccaggcatcaaataaaac
gaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactgg
ctcaccttcgggtgggcctttctgcgtttatagcactgaaggtcctcaatcgggtaggccggagcaggtgagtcgctct
ccaacgtgaagtttgtcagctatctgtagcccatctctgcatggcaacaggcacgcagcccgatgctgggcagatcctc
aactcgctgattaacagtattttgttaatcgatgacaacctggcgatccattacgccaaccctgccgcgcaacaactgc
tcgcccaaagctcccgcaaattgtttggtacaccgttaccggaactgttgagctacttctcattaaatatcgagctgat
gcaagaaagtctggaggcggggcaaggttttaccgataacgaagtgacgctggtcatcgacgggcgctcgcatatcctt
tctgtgacggcccagcgtatgccggacggcatgatcctgctggagatggctccgatggataaccagcgccgcttaagtc
aggaacagctacagcacgcccagcaggttactgcccgtgatttagtgcgcggcctggcacatgagattaaaaatccgct
tggcggtttacgtggcgcggcgcagctgctcagcaaagcgttacctgacccatcactactcgaatataccaaagtgatt
atcgaacaggcggaccggctgcgaaatctggtcgaccgtctgttggggccgcagctgcccggtacgcgcgttaccgaaa
gtattcacaaagtggctgaacgcgtggtaacgctggtgtcgatggaactgccggacaacgtgcggttgattcgtgatta
cgatcccagcctaccggaactggcgcacgacccggatcaaattgaacaggtcttgctgaatattgtgcgcaatgcgcta
caggcgctggggccggaaggcggtgaaatcattctgcgtacccgcaccgcgtttcaactgaccttacacggcgagcgct
accggctggcggcgcggattgatgtggaagataacgggccgggcattccgcctcatttgcaggatacgctgttttaccc
gatggtcagcggccgcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaa
attgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataaccaggcatcaaataaa
acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact
ggctcaccttcgggtgggcctttctgcgtttatacactggaaacatcaaggtcg