BBa_K2066121
type: ComponentDefinition
persistent identity: BBa K2066121
display id: BBa_K2066121
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: 2X TetO Binding Cassette(55aS) + NRII + TetR + sfGFP on UNS
created: 2016-10-13T11:00:00Z
modified: 2016-10-28T11:23:08Z
mutable provenance: To be completed
owned by: james
owned by: myers
mutable description: To be completed
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 31547
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: To be completed
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K2066541
component: BBa_K2066126
component: BBa_K2066539
component: BBa_B0015
component: BBa_K2066509
component: BBa_K2066540
component: BBa_K2066538
component: BBa_K2066532
component: BBa_K2066543
component: BBa_K2066542
component: BBa_I739101
component: BBa_K2066546
component: BBa_K2066547
component: BBa_K2066545
component: BBa_K2066544
sequence annotation: BBa_K2066541
sequence annotation: BBa_I739101
sequence annotation: BBa_K2066542
sequence annotation: BBa_K2066543
sequence annotation: BBa_K2066532
sequence annotation: BBa_K2066544
sequence annotation: BBa_K2066545
sequence annotation: BBa_K2066547
sequence annotation: BBa_K2066546
sequence annotation: BBa_K2066509
sequence annotation: BBa_B0015
sequence annotation: BBa_K2066539
sequence annotation: BBa_K2066126
sequence annotation: BBa_K2066538
sequence annotation: BBa_K2066540
sequence: https://synbiohub.org/public/igem/BBa_K2066121_sequence/1

BBa_K2066541
type: Component
persistent identity: component2523387
display id: component2523387
version: 1
top level: BBa_K2066121
definition: BBa_K2066541
access: public

BBa_K2066126
type: Component
persistent identity: component2523411
display id: component2523411
version: 1
top level: BBa_K2066121
definition: BBa_K2066126
access: public

BBa_K2066539
type: Component
persistent identity: component2523409
display id: component2523409
version: 1
top level: BBa_K2066121
definition: BBa_K2066539
access: public

BBa_B0015
type: Component
persistent identity: component2523408
display id: component2523408
version: 1
top level: BBa_K2066121
definition: BBa_B0015
access: public

BBa_K2066509
type: Component
persistent identity: component2523401
display id: component2523401
version: 1
top level: BBa_K2066121
definition: BBa_K2066509
access: public

BBa_K2066540
type: Component
persistent identity: component2523420
display id: component2523420
version: 1
top level: BBa_K2066121
definition: BBa_K2066540
access: public

BBa_B0015
type: Component
persistent identity: component2523419
display id: component2523419
version: 1
top level: BBa_K2066121
definition: BBa_B0015
access: public

BBa_K2066538
type: Component
persistent identity: component2523412
display id: component2523412
version: 1
top level: BBa_K2066121
definition: BBa_K2066538
access: public

BBa_K2066532
type: Component
persistent identity: component2523396
display id: component2523396
version: 1
top level: BBa_K2066121
definition: BBa_K2066532
access: public

BBa_K2066543
type: Component
persistent identity: component2523395
display id: component2523395
version: 1
top level: BBa_K2066121
definition: BBa_K2066543
access: public

BBa_K2066542
type: Component
persistent identity: component2523394
display id: component2523394
version: 1
top level: BBa_K2066121
definition: BBa_K2066542
access: public

BBa_I739101
type: Component
persistent identity: component2523393
display id: component2523393
version: 1
top level: BBa_K2066121
definition: BBa_I739101
access: public

BBa_K2066546
type: Component
persistent identity: component2523400
display id: component2523400
version: 1
top level: BBa_K2066121
definition: BBa_K2066546
access: public

BBa_K2066547
type: Component
persistent identity: component2523399
display id: component2523399
version: 1
top level: BBa_K2066121
definition: BBa_K2066547
access: public

BBa_K2066545
type: Component
persistent identity: component2523398
display id: component2523398
version: 1
top level: BBa_K2066121
definition: BBa_K2066545
access: public

BBa_K2066544
type: Component
persistent identity: component2523397
display id: component2523397
version: 1
top level: BBa_K2066121
definition: BBa_K2066544
access: public

BBa_K2066541
type: SequenceAnnotation
persistent identity: annotation2523387
display id: annotation2523387
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523387/range2523387/1
component: BBa_K2066541

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type: Range
persistent identity: range2523387
display id: range2523387
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 1
end: 20
orientation: inline

BBa_I739101
type: SequenceAnnotation
persistent identity: annotation2523393
display id: annotation2523393
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523393/range2523393/1
component: BBa_I739101

https://synbiohub.org/public/igem/BBa_K2066121/annotation2523393/range2523393/1
type: Range
persistent identity: range2523393
display id: range2523393
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 21
end: 103
orientation: inline

BBa_K2066542
type: SequenceAnnotation
persistent identity: annotation2523394
display id: annotation2523394
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523394/range2523394/1
component: BBa_K2066542

https://synbiohub.org/public/igem/BBa_K2066121/annotation2523394/range2523394/1
type: Range
persistent identity: range2523394
display id: range2523394
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 104
end: 123
orientation: inline

BBa_K2066543
type: SequenceAnnotation
persistent identity: annotation2523395
display id: annotation2523395
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523395/range2523395/1
component: BBa_K2066543

https://synbiohub.org/public/igem/BBa_K2066121/annotation2523395/range2523395/1
type: Range
persistent identity: range2523395
display id: range2523395
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 124
end: 170
orientation: inline

BBa_K2066532
type: SequenceAnnotation
persistent identity: annotation2523396
display id: annotation2523396
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523396/range2523396/1
component: BBa_K2066532

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type: Range
persistent identity: range2523396
display id: range2523396
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 171
end: 258
orientation: inline

BBa_K2066544
type: SequenceAnnotation
persistent identity: annotation2523397
display id: annotation2523397
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523397/range2523397/1
component: BBa_K2066544

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type: Range
persistent identity: range2523397
display id: range2523397
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 259
end: 288
orientation: inline

BBa_K2066545
type: SequenceAnnotation
persistent identity: annotation2523398
display id: annotation2523398
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523398/range2523398/1
component: BBa_K2066545

https://synbiohub.org/public/igem/BBa_K2066121/annotation2523398/range2523398/1
type: Range
persistent identity: range2523398
display id: range2523398
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 289
end: 310
orientation: inline

BBa_K2066547
type: SequenceAnnotation
persistent identity: annotation2523399
display id: annotation2523399
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523399/range2523399/1
component: BBa_K2066547

https://synbiohub.org/public/igem/BBa_K2066121/annotation2523399/range2523399/1
type: Range
persistent identity: range2523399
display id: range2523399
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 311
end: 453
orientation: inline

BBa_K2066546
type: SequenceAnnotation
persistent identity: annotation2523400
display id: annotation2523400
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523400/range2523400/1
component: BBa_K2066546

https://synbiohub.org/public/igem/BBa_K2066121/annotation2523400/range2523400/1
type: Range
persistent identity: range2523400
display id: range2523400
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 455
end: 1948
orientation: inline

BBa_K2066509
type: SequenceAnnotation
persistent identity: annotation2523401
display id: annotation2523401
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523401/range2523401/1
component: BBa_K2066509

https://synbiohub.org/public/igem/BBa_K2066121/annotation2523401/range2523401/1
type: Range
persistent identity: range2523401
display id: range2523401
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 1949
end: 2668
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2523408
display id: annotation2523408
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523408/range2523408/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2066121/annotation2523408/range2523408/1
type: Range
persistent identity: range2523408
display id: range2523408
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 2669
end: 2797
orientation: inline

BBa_K2066539
type: SequenceAnnotation
persistent identity: annotation2523409
display id: annotation2523409
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523409/range2523409/1
component: BBa_K2066539

https://synbiohub.org/public/igem/BBa_K2066121/annotation2523409/range2523409/1
type: Range
persistent identity: range2523409
display id: range2523409
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 2798
end: 2817
orientation: inline

BBa_K2066126
type: SequenceAnnotation
persistent identity: annotation2523411
display id: annotation2523411
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523411/range2523411/1
component: BBa_K2066126

https://synbiohub.org/public/igem/BBa_K2066121/annotation2523411/range2523411/1
type: Range
persistent identity: range2523411
display id: range2523411
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 2818
end: 2884
orientation: inline

BBa_K2066538
type: SequenceAnnotation
persistent identity: annotation2523412
display id: annotation2523412
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523412/range2523412/1
component: BBa_K2066538

https://synbiohub.org/public/igem/BBa_K2066121/annotation2523412/range2523412/1
type: Range
persistent identity: range2523412
display id: range2523412
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 2885
end: 3934
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2523419
display id: annotation2523419
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523419/range2523419/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2066121/annotation2523419/range2523419/1
type: Range
persistent identity: range2523419
display id: range2523419
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 3935
end: 4063
orientation: inline

BBa_K2066540
type: SequenceAnnotation
persistent identity: annotation2523420
display id: annotation2523420
version: 1
top level: BBa_K2066121
location: https://synbiohub.org/public/igem/BBa_K2066121/annotation2523420/range2523420/1
component: BBa_K2066540

https://synbiohub.org/public/igem/BBa_K2066121/annotation2523420/range2523420/1
type: Range
persistent identity: range2523420
display id: range2523420
version: 1
top level: BBa_K2066121
direction: direction/reverse
start: 4064
end: 4083
orientation: inline

BBa_I739101
type: ComponentDefinition
persistent identity: BBa I739101
display id: BBa_I739101
version: 1
was generated by: iGEM to SBOL conversion
description: Double Promoter (constitutive / TetR, negative)
created: 2007-10-11T11:00:00Z
modified: 2015-08-31T04:08:01Z
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
mutable description: sdfasdf
discontinued: false
dominant: false
experience: experience/None
group u list: _124_
m user id: 0
owner id: 2183
owning group id: 62
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: asdf
star: false
creator: Stefan Luzi
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: J23100
sequence annotation: Scar
sequence annotation: TetR 1
sequence annotation: R0040 (2x TetR 1)
sequence: https://synbiohub.org/public/igem/BBa_I739101_sequence/1

J23100
type: SequenceAnnotation
persistent identity: annotation1946100
display id: annotation1946100
version: 1
top level: BBa_I739101
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946100/range1946100/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I739101/annotation1946100/range1946100/1
type: Range
persistent identity: range1946100
display id: range1946100
version: 1
top level: BBa_I739101
direction: direction/forward
start: 1
end: 35
orientation: inline

Scar
type: SequenceAnnotation
persistent identity: annotation1946099
display id: annotation1946099
version: 1
top level: BBa_I739101
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946099/range1946099/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_I739101/annotation1946099/range1946099/1
type: Range
persistent identity: range1946099
display id: range1946099
version: 1
top level: BBa_I739101
direction: direction/forward
start: 36
end: 43
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946102
display id: annotation1946102
version: 1
top level: BBa_I739101
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946102/range1946102/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I739101/annotation1946102/range1946102/1
type: Range
persistent identity: range1946102
display id: range1946102
version: 1
top level: BBa_I739101
direction: direction/forward
start: 44
end: 63
orientation: inline

R0040 (2x TetR 1)
type: SequenceAnnotation
persistent identity: annotation1946257
display id: annotation1946257
version: 1
top level: BBa_I739101
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946257/range1946257/1
role: feature/operator
role: SO:0000057

https://synbiohub.org/public/igem/BBa_I739101/annotation1946257/range1946257/1
type: Range
persistent identity: range1946257
display id: range1946257
version: 1
top level: BBa_I739101
direction: direction/forward
start: 44
end: 83
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1946101
display id: annotation1946101
version: 1
top level: BBa_I739101
location: https://synbiohub.org/public/igem/BBa_I739101/annotation1946101/range1946101/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I739101/annotation1946101/range1946101/1
type: Range
persistent identity: range1946101
display id: range1946101
version: 1
top level: BBa_I739101
direction: direction/forward
start: 64
end: 83
orientation: inline

BBa_K2066541
type: ComponentDefinition
persistent identity: BBa K2066541
display id: BBa_K2066541
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 2.1
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T06:25:46Z
mutable provenance: petme
owned by: james
owned by: myers
mutable description: tm
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: pt
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K2066541_sequence/1

BBa_K2066542
type: ComponentDefinition
persistent identity: BBa K2066542
display id: BBa_K2066542
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 2.2
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T06:27:20Z
mutable provenance: pet
owned by: james
owned by: myers
mutable description: ptme
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: mep
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066542_sequence/1

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_K2066509
type: ComponentDefinition
persistent identity: BBa K2066509
display id: BBa_K2066509
version: 1
was generated by: iGEM to SBOL conversion
description: sfGFP
created: 2016-08-30T11:00:00Z
modified: 2016-10-19T02:54:25Z
mutable provenance: The sequence for this sfGFP reporter gene is modified from Lou et al. Supplement section V. This is the sequence of superfolder GFP BBa_I746916, but with four codon modifications to match WM16_015: at position 441, G->T. At 446, C->T. At 495, T->C. At 562, C->A.
owned by: james
owned by: myers
mutable description: The part is a reporter used for K2066014.
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 31541
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Design inspired by Lou et. al. 2012
star: false
creator: Likhitha Kolla
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence: https://synbiohub.org/public/igem/BBa_K2066509_sequence/1

BBa_K2066126
type: ComponentDefinition
persistent identity: BBa K2066126
display id: BBa_K2066126
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: NRII2302 Upstream Regulatory Region with Promoter
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:55:12Z
mutable provenance: temp
owned by: james
owned by: myers
mutable description: temp
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: temp
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_K2066018
sequence: https://synbiohub.org/public/igem/BBa_K2066126_sequence/1

BBa_K2066018
type: SequenceAnnotation
persistent identity: annotation2500695
display id: annotation2500695
version: 1
top level: BBa_K2066126
location: https://synbiohub.org/public/igem/BBa_K2066126/annotation2500695/range2500695/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K2066126/annotation2500695/range2500695/1
type: Range
persistent identity: range2500695
display id: range2500695
version: 1
top level: BBa_K2066126
direction: direction/reverse
start: 1
end: 40
orientation: inline

BBa_K2066532
type: ComponentDefinition
persistent identity: BBa K2066532
display id: BBa_K2066532
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: 2X TetO Binding Cassette (55AS)
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:15:03Z
mutable provenance: .
owned by: james
owned by: myers
mutable description: /
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: .
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066532_sequence/1

BBa_K2066538
type: ComponentDefinition
persistent identity: BBa K2066538
display id: BBa_K2066538
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: NRII2302 Mutant Coding
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:51:49Z
mutable provenance: te
owned by: james
owned by: myers
mutable description: temp
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: mptem
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K2066538_sequence/1

BBa_K2066539
type: ComponentDefinition
persistent identity: BBa K2066539
display id: BBa_K2066539
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 3.1
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T02:36:08Z
mutable provenance: temp
owned by: james
owned by: myers
mutable description: temp
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: tme
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066539_sequence/1

BBa_K2066540
type: ComponentDefinition
persistent identity: BBa K2066540
display id: BBa_K2066540
version: 1
was generated by: iGEM to SBOL conversion
description: UNS 3.2
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T06:23:08Z
mutable provenance: tem
owned by: james
owned by: myers
mutable description: temp
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31544
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: p
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066540_sequence/1

BBa_K2066543
type: ComponentDefinition
persistent identity: BBa K2066543
display id: BBa_K2066543
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: Enhancer/NRI binding sites
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T04:20:03Z
mutable provenance: mep
owned by: james
owned by: myers
mutable description: tmept
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: temp
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K2066543_sequence/1

BBa_K2066544
type: ComponentDefinition
persistent identity: BBa K2066544
display id: BBa_K2066544
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: glnAp2 promoter
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T04:48:44Z
mutable provenance: temp
owned by: james
owned by: myers
mutable description: temp
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: temp
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K2066544_sequence/1

BBa_K2066545
type: ComponentDefinition
persistent identity: BBa K2066545
display id: BBa_K2066545
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: LacI binding site (LacO)
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:05:36Z
mutable provenance: temp
owned by: james
owned by: myers
mutable description: temp
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: tem
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence: https://synbiohub.org/public/igem/BBa_K2066545_sequence/1

BBa_K2066546
type: ComponentDefinition
persistent identity: BBa K2066546
display id: BBa_K2066546
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: glnG (NRI)
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:07:26Z
mutable provenance: mept
owned by: james
owned by: myers
mutable description: tempt
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: metp
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_K2066546_sequence/1

BBa_K2066547
type: ComponentDefinition
persistent identity: BBa K2066547
display id: BBa_K2066547
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic Enhancer Project: Spacer between Promoter and RBS
created: 2016-10-13T11:00:00Z
modified: 2016-10-19T05:11:55Z
mutable provenance: petm
owned by: james
owned by: myers
mutable description: ptem
discontinued: false
dominant: false
experience: experience/None
group u list: _2534_
m user id: 31541
owner id: 27645
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: ep
star: false
creator: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_K2066547_sequence/1

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K2066121_sequence/1
type: Sequence
persistent identity: BBa K2066121 sequence
display id: BBa_K2066121_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacggattgacggctagctcagtcctaggtacagtgctagctactagagtccctatcagtgatagagattccctatcagtgatagagataacaaacgcagaatccaagctcacaattgcaccaacatggtgcttaatgtttccattgaagcactatattggtgcaacattgtcttcgacgctagcagcgcgcggagattctagccccttttcgaggaacgtccccatgataacgccttttaggggcaatttaaaagttggcacagatttcgctttatcttttttaattgtgagcgctcacaattaagcttggcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataaacgtttatgcaacgagggatagtctgggtagtcgatgacgatagttccatccgttgggtgcttgaacgtgcgctcgctggggcaggtttaacctgtacgacgtttgagaacggcgcagaagtgctggaggcgctggcgagcaaaacgccggatgtgctgctttcagatatccgtatgccgggaatggacgggctggcgctgctcaagcagattaaacagcgccatccaatgcttccggtcatcattatgaccgcacattccgatctggatgctgccgtcagcgcctatcaacaaggggcgtttgattatctgcccaaaccgtttgatatcgacgaagcagtggcgctggttgagcgcgctatcagtcattaccaggaacagcagcagccgcgtaatgttcagcttaacggcccaacgaccgatatcatcggcgaagcgccagccatgcaggacgtgttccgtattatcggtcggctttcgcgttcttctattagcgtgctgattaacggcgaatccggcaccggtaaagaactggtcgctcatgccctgcatcgccacagtccgcgcgccaaagcgccgtttatcgcgctgaatatggcagctatcccaaaagatttgatcgaatcagaactgtttggccacgagaaaggcgcgtttactggcgcgaataccattcgtcaggggcgttttgaacaggccgatggcggtacattattcctcgacgaaattggtgatatgccgctggatgtgcagacgcgtttgctgcgcgtgctggcagacggtcagttttaccgcgttggcggctatgcgccggtgaaagtggatgtgcggattatcgctgccactcaccagaatctcgaacagcgagtgcaggaaggtaagttccgtgaggatctgttccaccgcctgaacgttatccgcgttcatctgccgccgctgcgcgaacgtcgggaagatattccccgtctggcgcgccattttttacaggttgccgcgcgcgaactgggcgtagaagcgaagttactgcatccggaaaccgaagctgctctgacgcgtctggcgtggccaggcaacgtgcgccagctggaaaacacctgccgctggctaacggtgatggccgccgggcaggaagtgttgattcaggatttgcccggcgaactgtttgaatcaacggttgcggagagtacttcgcaaatgcaaccggacagctgggcgacgcttcttgcgcagtgggcagacagagcgctgcgttccggtcatcaaaatctgctttccgaagcgcagccagagctggagcggacgttactgacgaccgcgttgcgacatacgcaggggcataaacaggaagcggcgcggctactcggctggggccgcaacaccctgacgcgtaagttaaaagagctggggatggagtgattcacagcttgtgtgtaaagattgattattgagcgcaaattgctggtattttacgctttactgttccaggaggaaaaaaatgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I739101_sequence/1
type: Sequence
persistent identity: BBa I739101 sequence
display id: BBa_I739101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacggctagctcagtcctaggtacagtgctagctactagagtccctatcagtgatagagattccctatcagtgatagagat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066541_sequence/1
type: Sequence
persistent identity: BBa K2066541 sequence
display id: BBa_K2066541_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgggagttcgtagacgga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066542_sequence/1
type: Sequence
persistent identity: BBa K2066542 sequence
display id: BBa_K2066542_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aacaaacgcagaatccaagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066509_sequence/1
type: Sequence
persistent identity: BBa K2066509 sequence
display id: BBa_K2066509_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggcgaagagctgttcactggtgtcgtccctattctggtggaactggatggtgatgtcaacggtcataagttttccgtgcgtggcgagggtgaaggtgacgcaactaatggtaaactgacgctgaagttcatctgtactactggtaaactgccggtaccttggccgactctggtaacgacgctgacttatggtgttcagtgctttgctcgttatccggaccatatgaagcagcatgacttcttcaagtccgccatgccggaaggctatgtgcaggaacgcacgatttcctttaaggatgacggcacgtacaaaacgcgtgcggaagtgaaatttgaaggcgataccctggtaaaccgcattgagctgaaaggcattgactttaaagaagacggcaatatcctgggccataagctggaatacaattttaacagccacaatgtgtacattaccgcagataaacaaaaaaatggcattaaagcgaatttcaaaattcgccacaacgtggaggatggcagcgtgcagctggctgatcactaccagcaaaacactccaatcggtgatggtcctgttctgctgccagacaatcactatctgagcacgcaaagcgttctgtctaaagatccgaacgagaaacgcgatcatatggttctgctggagttcgtaaccgcagcgggcatcacgcatggtatggatgaactgtacaaatgatga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066126_sequence/1
type: Sequence
persistent identity: BBa K2066126 sequence
display id: BBa_K2066126_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggtaggccggagcaggtgagtcgctctccaacgtgaagtttgtcagctatctgtagcccatctctgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066532_sequence/1
type: Sequence
persistent identity: BBa K2066532 sequence
display id: BBa_K2066532_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attggtgcaacattgtcttcgacgctagcagcgcgcggagattctagccccttttcgaggaacgtccccatgataacgccttttaggg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066538_sequence/1
type: Sequence
persistent identity: BBa K2066538 sequence
display id: BBa_K2066538_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcaacaggcacgcagcccgatgctgggcagatcctcaactcgctgattaacagtattttgttaatcgatgacaacctggcgatccattacgccaaccctgccgcgcaacaactgctcgcccaaagctcccgcaaattgtttggtacaccgttaccggaactgttgagctacttctcattaaatatcgagctgatgcaagaaagtctggaggcggggcaaggttttaccgataacgaagtgacgctggtcatcgacgggcgctcgcatatcctttctgtgacggcccagcgtatgccggacggcatgatcctgctggagatggctccgatggataaccagcgccgcttaagtcaggaacagctacagcacgcccagcaggttactgcccgtgatttagtgcgcggcctggcacatgagattaaaaatccgcttggcggtttacgtggcgcggcgcagctgctcagcaaagcgttacctgacccatcactactcgaatataccaaagtgattatcgaacaggcggaccggctgcgaaatctggtcgaccgtctgttggggccgcagctgcccggtacgcgcgttaccgaaagtattcacaaagtggctgaacgcgtggtaacgctggtgtcgatggaactgccggacaacgtgcggttgattcgtgattacgatcccagcctaccggaactggcgcacgacccggatcaaattgaacaggtcttgctgaatattgtgcgcaatgcgctacaggcgctggggccggaaggcggtgaaatcattctgcgtacccgcaccgcgtttcaactgaccttacacggcgagcgctaccggctggcggcgcggattgatgtggaagataacgggccgggcattccgcctcatttgcaggatacgctgttttacccgatggtcagcggccgcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066539_sequence/1
type: Sequence
persistent identity: BBa K2066539 sequence
display id: BBa_K2066539_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcactgaaggtcctcaatcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066540_sequence/1
type: Sequence
persistent identity: BBa K2066540 sequence
display id: BBa_K2066540_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cactggaaacatcaaggtcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066543_sequence/1
type: Sequence
persistent identity: BBa K2066543 sequence
display id: BBa_K2066543_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacaattgcaccaacatggtgcttaatgtttccattgaagcactat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066544_sequence/1
type: Sequence
persistent identity: BBa K2066544 sequence
display id: BBa_K2066544_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaatttaaaagttggcacagatttcgctt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066545_sequence/1
type: Sequence
persistent identity: BBa K2066545 sequence
display id: BBa_K2066545_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tatcttttttaattgtgagcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066546_sequence/1
type: Sequence
persistent identity: BBa K2066546 sequence
display id: BBa_K2066546_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cgtttatgcaacgagggatagtctgggtagtcgatgacgatagttccatccgttgggtgcttgaacgtgcgctcgctggggcaggtttaacctgtacgacgtttgagaacggcgcagaagtgctggaggcgctggcgagcaaaacgccggatgtgctgctttcagatatccgtatgccgggaatggacgggctggcgctgctcaagcagattaaacagcgccatccaatgcttccggtcatcattatgaccgcacattccgatctggatgctgccgtcagcgcctatcaacaaggggcgtttgattatctgcccaaaccgtttgatatcgacgaagcagtggcgctggttgagcgcgctatcagtcattaccaggaacagcagcagccgcgtaatgttcagcttaacggcccaacgaccgatatcatcggcgaagcgccagccatgcaggacgtgttccgtattatcggtcggctttcgcgttcttctattagcgtgctgattaacggcgaatccggcaccggtaaagaactggtcgctcatgccctgcatcgccacagtccgcgcgccaaagcgccgtttatcgcgctgaatatggcagctatcccaaaagatttgatcgaatcagaactgtttggccacgagaaaggcgcgtttactggcgcgaataccattcgtcaggggcgttttgaacaggccgatggcggtacattattcctcgacgaaattggtgatatgccgctggatgtgcagacgcgtttgctgcgcgtgctggcagacggtcagttttaccgcgttggcggctatgcgccggtgaaagtggatgtgcggattatcgctgccactcaccagaatctcgaacagcgagtgcaggaaggtaagttccgtgaggatctgttccaccgcctgaacgttatccgcgttcatctgccgccgctgcgcgaacgtcgggaagatattccccgtctggcgcgccattttttacaggttgccgcgcgcgaactgggcgtagaagcgaagttactgcatccggaaaccgaagctgctctgacgcgtctggcgtggccaggcaacgtgcgccagctggaaaacacctgccgctggctaacggtgatggccgccgggcaggaagtgttgattcaggatttgcccggcgaactgtttgaatcaacggttgcggagagtacttcgcaaatgcaaccggacagctgggcgacgcttcttgcgcagtgggcagacagagcgctgcgttccggtcatcaaaatctgctttccgaagcgcagccagagctggagcggacgttactgacgaccgcgttgcgacatacgcaggggcataaacaggaagcggcgcggctactcggctggggccgcaacaccctgacgcgtaagttaaaagagctggggatggagtgattcacagcttgtgtgtaaagattgattattgagcgcaaattgctggtattttacgctttactgttccaggaggaaaaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2066547_sequence/1
type: Sequence
persistent identity: BBa K2066547 sequence
display id: BBa_K2066547_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacaattaagcttggcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z