LOCUS BBa_K2066122 4144 bp DNA linear 22-Nov-2024 DEFINITION Synthetic Enhancer Project: 3X TetO Binding Cassette(52S) + NRII + TetR + sfGFP on UNS ACCESSION BBa_K2066122 VERSION BBa_K2066122.1 FEATURES Location/Qualifiers misc_feature 1..20 /label=BBa_K2066541 misc_feature 56..63 /label=Scar promoter 21..55 /label=J23100 misc_feature 84..103 /label=TetR 1 misc_feature 64..83 /label=TetR 1 misc_feature 64..103 /label=R0040 (2x TetR 1) promoter 21..103 /label=BBa_I739101 misc_feature 104..123 /label=BBa_K2066542 misc_feature 124..170 /label=BBa_K2066543 misc_feature 171..319 /label=BBa_K2066531 promoter 320..349 /label=BBa_K2066544 misc_feature 350..371 /label=BBa_K2066545 misc_feature 372..514 /label=BBa_K2066547 CDS 516..2009 /label=BBa_K2066546 misc_feature 2010..2729 /label=BBa_K2066509 stem_loop 2741..2784 /label=stem_loop misc_feature 2730..2809 /label=BBa_B0010 terminator 2730..2809 /label=BBa_B0010 stem_loop 2825..2844 /label=T7 TE misc_feature 2851..2851 /label=stop polyA_site 2845..2858 /label=polya misc_feature 2818..2858 /label=BBa_B0012 terminator 2818..2858 /label=BBa_B0012 terminator 2730..2858 /label=BBa_B0015 misc_feature 2859..2878 /label=BBa_K2066539 misc_feature 2879..2918 /label=BBa_K2066018 promoter 2879..2945 /label=BBa_K2066126 CDS 2946..3995 /label=BBa_K2066538 stem_loop 4007..4050 /label=stem_loop misc_feature 3996..4075 /label=BBa_B0010 terminator 3996..4075 /label=BBa_B0010 stem_loop 4091..4110 /label=T7 TE misc_feature 4117..4117 /label=stop polyA_site 4111..4124 /label=polya misc_feature 4084..4124 /label=BBa_B0012 terminator 4084..4124 /label=BBa_B0012 terminator 3996..4124 /label=BBa_B0015 misc_feature 4125..4144 /label=BBa_K2066540 ORIGIN 1 gctgggagtt cgtagacgga ttgacggcta gctcagtcct aggtacagtg ctagctacta 61 gagtccctat cagtgataga gattccctat cagtgataga gataacaaac gcagaatcca 121 agctcacaat tgcaccaaca tggtgcttaa tgtttccatt gaagcactat attggtgcaa 181 cattgtcttc gacgctagct ctctatcact gatagggatg tcggaggctc gcggactcta 241 tcaatgatag agtctcgagt ctggccggat ctctatcact gatagggagc tagcgtcccc 301 atgataacgc cttttagggg caatttaaaa gttggcacag atttcgcttt atctttttta 361 attgtgagcg ctcacaatta agcttggcga aggtggcacc gggcttggct tatccatcgc 421 tcgtaatttg attgatcagc attcaggcaa aattgaattt accagttggc cagggcatac 481 cgagttctcg gtttacctgc ctatcaggaa ataaacgttt atgcaacgag ggatagtctg 541 ggtagtcgat gacgatagtt ccatccgttg ggtgcttgaa cgtgcgctcg ctggggcagg 601 tttaacctgt acgacgtttg agaacggcgc agaagtgctg gaggcgctgg cgagcaaaac 661 gccggatgtg ctgctttcag atatccgtat gccgggaatg gacgggctgg cgctgctcaa 721 gcagattaaa cagcgccatc caatgcttcc ggtcatcatt atgaccgcac attccgatct 781 ggatgctgcc gtcagcgcct atcaacaagg ggcgtttgat tatctgccca aaccgtttga 841 tatcgacgaa gcagtggcgc tggttgagcg cgctatcagt cattaccagg aacagcagca 901 gccgcgtaat gttcagctta acggcccaac gaccgatatc atcggcgaag cgccagccat 961 gcaggacgtg ttccgtatta tcggtcggct ttcgcgttct tctattagcg tgctgattaa 1021 cggcgaatcc ggcaccggta aagaactggt cgctcatgcc ctgcatcgcc acagtccgcg 1081 cgccaaagcg ccgtttatcg cgctgaatat ggcagctatc ccaaaagatt tgatcgaatc 1141 agaactgttt ggccacgaga aaggcgcgtt tactggcgcg aataccattc gtcaggggcg 1201 ttttgaacag gccgatggcg gtacattatt cctcgacgaa attggtgata tgccgctgga 1261 tgtgcagacg cgtttgctgc gcgtgctggc agacggtcag ttttaccgcg ttggcggcta 1321 tgcgccggtg aaagtggatg tgcggattat cgctgccact caccagaatc tcgaacagcg 1381 agtgcaggaa ggtaagttcc gtgaggatct gttccaccgc ctgaacgtta tccgcgttca 1441 tctgccgccg ctgcgcgaac gtcgggaaga tattccccgt ctggcgcgcc attttttaca 1501 ggttgccgcg cgcgaactgg gcgtagaagc gaagttactg catccggaaa ccgaagctgc 1561 tctgacgcgt ctggcgtggc caggcaacgt gcgccagctg gaaaacacct gccgctggct 1621 aacggtgatg gccgccgggc aggaagtgtt gattcaggat ttgcccggcg aactgtttga 1681 atcaacggtt gcggagagta cttcgcaaat gcaaccggac agctgggcga cgcttcttgc 1741 gcagtgggca gacagagcgc tgcgttccgg tcatcaaaat ctgctttccg aagcgcagcc 1801 agagctggag cggacgttac tgacgaccgc gttgcgacat acgcaggggc ataaacagga 1861 agcggcgcgg ctactcggct ggggccgcaa caccctgacg cgtaagttaa aagagctggg 1921 gatggagtga ttcacagctt gtgtgtaaag attgattatt gagcgcaaat tgctggtatt 1981 ttacgcttta ctgttccagg aggaaaaaaa tgcgtaaagg cgaagagctg ttcactggtg 2041 tcgtccctat tctggtggaa ctggatggtg atgtcaacgg tcataagttt tccgtgcgtg 2101 gcgagggtga aggtgacgca actaatggta aactgacgct gaagttcatc tgtactactg 2161 gtaaactgcc ggtaccttgg ccgactctgg taacgacgct gacttatggt gttcagtgct 2221 ttgctcgtta tccggaccat atgaagcagc atgacttctt caagtccgcc atgccggaag 2281 gctatgtgca ggaacgcacg atttccttta aggatgacgg cacgtacaaa acgcgtgcgg 2341 aagtgaaatt tgaaggcgat accctggtaa accgcattga gctgaaaggc attgacttta 2401 aagaagacgg caatatcctg ggccataagc tggaatacaa ttttaacagc cacaatgtgt 2461 acattaccgc agataaacaa aaaaatggca ttaaagcgaa tttcaaaatt cgccacaacg 2521 tggaggatgg cagcgtgcag ctggctgatc actaccagca aaacactcca atcggtgatg 2581 gtcctgttct gctgccagac aatcactatc tgagcacgca aagcgttctg tctaaagatc 2641 cgaacgagaa acgcgatcat atggttctgc tggagttcgt aaccgcagcg ggcatcacgc 2701 atggtatgga tgaactgtac aaatgatgac caggcatcaa ataaaacgaa aggctcagtc 2761 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 2821 cactggctca ccttcgggtg ggcctttctg cgtttatagc actgaaggtc ctcaatcggg 2881 taggccggag caggtgagtc gctctccaac gtgaagtttg tcagctatct gtagcccatc 2941 tctgcatggc aacaggcacg cagcccgatg ctgggcagat cctcaactcg ctgattaaca 3001 gtattttgtt aatcgatgac aacctggcga tccattacgc caaccctgcc gcgcaacaac 3061 tgctcgccca aagctcccgc aaattgtttg gtacaccgtt accggaactg ttgagctact 3121 tctcattaaa tatcgagctg atgcaagaaa gtctggaggc ggggcaaggt tttaccgata 3181 acgaagtgac gctggtcatc gacgggcgct cgcatatcct ttctgtgacg gcccagcgta 3241 tgccggacgg catgatcctg ctggagatgg ctccgatgga taaccagcgc cgcttaagtc 3301 aggaacagct acagcacgcc cagcaggtta ctgcccgtga tttagtgcgc ggcctggcac 3361 atgagattaa aaatccgctt ggcggtttac gtggcgcggc gcagctgctc agcaaagcgt 3421 tacctgaccc atcactactc gaatatacca aagtgattat cgaacaggcg gaccggctgc 3481 gaaatctggt cgaccgtctg ttggggccgc agctgcccgg tacgcgcgtt accgaaagta 3541 ttcacaaagt ggctgaacgc gtggtaacgc tggtgtcgat ggaactgccg gacaacgtgc 3601 ggttgattcg tgattacgat cccagcctac cggaactggc gcacgacccg gatcaaattg 3661 aacaggtctt gctgaatatt gtgcgcaatg cgctacaggc gctggggccg gaaggcggtg 3721 aaatcattct gcgtacccgc accgcgtttc aactgacctt acacggcgag cgctaccggc 3781 tggcggcgcg gattgatgtg gaagataacg ggccgggcat tccgcctcat ttgcaggata 3841 cgctgtttta cccgatggtc agcggccgcg aaggtggcac cgggcttggc ttatccatcg 3901 ctcgtaattt gattgatcag cattcaggca aaattgaatt taccagttgg ccagggcata 3961 ccgagttctc ggtttacctg cctatcagga aataaccagg catcaaataa aacgaaaggc 4021 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 4081 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatacactgg aaacatcaag 4141 gtcg //