BBa_K2066539
1
BBa_K2066539
UNS 3.1
2016-10-13T11:00:00Z
2016-10-19T02:36:08Z
temp
temp
false
false
_2534_
31541
27645
9
false
tme
false
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
BBa_K2066543
1
BBa_K2066543
Synthetic Enhancer Project: Enhancer/NRI binding sites
2016-10-13T11:00:00Z
2016-10-19T04:20:03Z
mep
tmept
false
false
_2534_
31541
27645
9
false
temp
false
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
BBa_K2066546
1
BBa_K2066546
Synthetic Enhancer Project: glnG (NRI)
2016-10-13T11:00:00Z
2016-10-19T05:07:26Z
mept
tempt
false
false
_2534_
31541
27645
9
false
metp
false
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
BBa_K2066547
1
BBa_K2066547
Synthetic Enhancer Project: Spacer between Promoter and RBS
2016-10-13T11:00:00Z
2016-10-19T05:11:55Z
petm
ptem
false
false
_2534_
31541
27645
9
false
ep
false
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
BBa_K2066518
1
BBa_K2066518
mCherry (with start codon)
2016-10-07T11:00:00Z
2016-10-08T10:50:28Z
Modified from the mCherry used in K2066028 and K2066029 to include the start codon. This modified mCherry is used in the construction of K2066040 and K2066041.
false
false
_2534_
31544
31544
9
false
The start codon was removed in K2066028 and K2066029 to follow the design from Synthetic Analog Computation in Living Cells (Daniel et al.) where the codon bias was being optimized.
false
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
BBa_K2066126
1
BBa_K2066126
Synthetic Enhancer Project: NRII2302 Upstream Regulatory Region with Promoter
2016-10-13T11:00:00Z
2016-10-19T05:55:12Z
temp
temp
false
false
_2534_
31541
27645
9
false
temp
false
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
annotation2500695
1
BBa_K2066018
range2500695
1
1
40
BBa_K2066538
1
BBa_K2066538
Synthetic Enhancer Project: NRII2302 Mutant Coding
2016-10-13T11:00:00Z
2016-10-19T05:51:49Z
te
temp
false
false
_2534_
31541
27645
9
false
mptem
false
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
BBa_I739101
1
BBa_I739101
Double Promoter (constitutive / TetR, negative)
2007-10-11T11:00:00Z
2015-08-31T04:08:01Z
Synthetic DNA
sdfasdf
false
false
_124_
0
2183
62
Not in stock
false
asdf
false
Stefan Luzi
annotation1946102
1
TetR 1
range1946102
1
44
63
annotation1946100
1
J23100
range1946100
1
1
35
annotation1946257
1
R0040 (2x TetR 1)
range1946257
1
44
83
annotation1946099
1
Scar
range1946099
1
36
43
annotation1946101
1
TetR 1
range1946101
1
64
83
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916612
1
BBa_B0012
range1916612
1
89
129
annotation1916610
1
BBa_B0010
range1916610
1
1
80
BBa_K2066540
1
BBa_K2066540
UNS 3.2
2016-10-13T11:00:00Z
2016-10-19T06:23:08Z
tem
temp
false
false
_2534_
31544
27645
9
false
p
false
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
BBa_K2066123
1
BBa_K2066123
Synthetic Enhancer Project: UNS 55as NRII TetR
2016-10-13T11:00:00Z
2016-10-28T10:18:02Z
To be completed
To be completed
true
false
_2534_
31541
31547
9
false
To be completed
false
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
component2523465
1
BBa_K2066543
component2523479
1
BBa_K2066539
component2523463
1
BBa_I739101
component2523481
1
BBa_K2066126
component2523478
1
BBa_B0015
component2523467
1
BBa_K2066544
component2523470
1
BBa_K2066546
component2523457
1
BBa_K2066541
component2523489
1
BBa_B0015
component2523490
1
BBa_K2066540
component2523464
1
BBa_K2066542
component2523468
1
BBa_K2066545
component2523471
1
BBa_K2066518
component2523482
1
BBa_K2066538
component2523469
1
BBa_K2066547
component2523466
1
BBa_K2066532
annotation2523457
1
BBa_K2066541
range2523457
1
1
20
annotation2523481
1
BBa_K2066126
range2523481
1
2812
2878
annotation2523478
1
BBa_B0015
range2523478
1
2663
2791
annotation2523482
1
BBa_K2066538
range2523482
1
2879
3928
annotation2523463
1
BBa_I739101
range2523463
1
21
103
annotation2523465
1
BBa_K2066543
range2523465
1
124
170
annotation2523466
1
BBa_K2066532
range2523466
1
171
258
annotation2523469
1
BBa_K2066547
range2523469
1
311
453
annotation2523468
1
BBa_K2066545
range2523468
1
289
310
annotation2523471
1
BBa_K2066518
range2523471
1
1949
2662
annotation2523479
1
BBa_K2066539
range2523479
1
2792
2811
annotation2523490
1
BBa_K2066540
range2523490
1
4058
4077
annotation2523467
1
BBa_K2066544
range2523467
1
259
288
annotation2523464
1
BBa_K2066542
range2523464
1
104
123
annotation2523489
1
BBa_B0015
range2523489
1
3929
4057
annotation2523470
1
BBa_K2066546
range2523470
1
455
1948
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K2066542
1
BBa_K2066542
UNS 2.2
2016-10-13T11:00:00Z
2016-10-19T06:27:20Z
pet
ptme
false
false
_2534_
31544
27645
9
false
mep
false
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
BBa_K2066544
1
BBa_K2066544
Synthetic Enhancer Project: glnAp2 promoter
2016-10-13T11:00:00Z
2016-10-19T04:48:44Z
temp
temp
false
false
_2534_
31541
27645
9
false
temp
false
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
BBa_K2066532
1
BBa_K2066532
Synthetic Enhancer Project: 2X TetO Binding Cassette (55AS)
2016-10-13T11:00:00Z
2016-10-19T05:15:03Z
.
/
false
false
_2534_
31541
27645
9
false
.
false
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K2066541
1
BBa_K2066541
UNS 2.1
2016-10-13T11:00:00Z
2016-10-19T06:25:46Z
petme
tm
false
false
_2534_
31544
27645
9
false
pt
false
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
BBa_K2066545
1
BBa_K2066545
Synthetic Enhancer Project: LacI binding site (LacO)
2016-10-13T11:00:00Z
2016-10-19T05:05:36Z
temp
temp
false
false
_2534_
31541
27645
9
false
tem
false
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
BBa_K2066541_sequence
1
gctgggagttcgtagacgga
BBa_K2066532_sequence
1
attggtgcaacattgtcttcgacgctagcagcgcgcggagattctagccccttttcgaggaacgtccccatgataacgccttttaggg
BBa_K2066539_sequence
1
gcactgaaggtcctcaatcg
BBa_K2066543_sequence
1
tcacaattgcaccaacatggtgcttaatgtttccattgaagcactat
BBa_K2066545_sequence
1
tatcttttttaattgtgagcgc
BBa_K2066540_sequence
1
cactggaaacatcaaggtcg
BBa_K2066544_sequence
1
gcaatttaaaagttggcacagatttcgctt
BBa_K2066542_sequence
1
aacaaacgcagaatccaagc
BBa_K2066123_sequence
1
gctgggagttcgtagacggattgacggctagctcagtcctaggtacagtgctagctactagagtccctatcagtgatagagattccctatcagtgatagagataacaaacgcagaatccaagctcacaattgcaccaacatggtgcttaatgtttccattgaagcactatattggtgcaacattgtcttcgacgctagcagcgcgcggagattctagccccttttcgaggaacgtccccatgataacgccttttaggggcaatttaaaagttggcacagatttcgctttatcttttttaattgtgagcgctcacaattaagcttggcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataaacgtttatgcaacgagggatagtctgggtagtcgatgacgatagttccatccgttgggtgcttgaacgtgcgctcgctggggcaggtttaacctgtacgacgtttgagaacggcgcagaagtgctggaggcgctggcgagcaaaacgccggatgtgctgctttcagatatccgtatgccgggaatggacgggctggcgctgctcaagcagattaaacagcgccatccaatgcttccggtcatcattatgaccgcacattccgatctggatgctgccgtcagcgcctatcaacaaggggcgtttgattatctgcccaaaccgtttgatatcgacgaagcagtggcgctggttgagcgcgctatcagtcattaccaggaacagcagcagccgcgtaatgttcagcttaacggcccaacgaccgatatcatcggcgaagcgccagccatgcaggacgtgttccgtattatcggtcggctttcgcgttcttctattagcgtgctgattaacggcgaatccggcaccggtaaagaactggtcgctcatgccctgcatcgccacagtccgcgcgccaaagcgccgtttatcgcgctgaatatggcagctatcccaaaagatttgatcgaatcagaactgtttggccacgagaaaggcgcgtttactggcgcgaataccattcgtcaggggcgttttgaacaggccgatggcggtacattattcctcgacgaaattggtgatatgccgctggatgtgcagacgcgtttgctgcgcgtgctggcagacggtcagttttaccgcgttggcggctatgcgccggtgaaagtggatgtgcggattatcgctgccactcaccagaatctcgaacagcgagtgcaggaaggtaagttccgtgaggatctgttccaccgcctgaacgttatccgcgttcatctgccgccgctgcgcgaacgtcgggaagatattccccgtctggcgcgccattttttacaggttgccgcgcgcgaactgggcgtagaagcgaagttactgcatccggaaaccgaagctgctctgacgcgtctggcgtggccaggcaacgtgcgccagctggaaaacacctgccgctggctaacggtgatggccgccgggcaggaagtgttgattcaggatttgcccggcgaactgtttgaatcaacggttgcggagagtacttcgcaaatgcaaccggacagctgggcgacgcttcttgcgcagtgggcagacagagcgctgcgttccggtcatcaaaatctgctttccgaagcgcagccagagctggagcggacgttactgacgaccgcgttgcgacatacgcaggggcataaacaggaagcggcgcggctactcggctggggccgcaacaccctgacgcgtaagttaaaagagctggggatggagtgattcacagcttgtgtgtaaagattgattattgagcgcaaattgctggtattttacgctttactgttccaggaggaaaaaaatggtgagcaagggcgaagaagataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatagcactgaaggtcctcaatcgggtaggccggagcaggtgagtcgctctccaacgtgaagtttgtcagctatctgtagcccatctctgcatggcaacaggcacgcagcccgatgctgggcagatcctcaactcgctgattaacagtattttgttaatcgatgacaacctggcgatccattacgccaaccctgccgcgcaacaactgctcgcccaaagctcccgcaaattgtttggtacaccgttaccggaactgttgagctacttctcattaaatatcgagctgatgcaagaaagtctggaggcggggcaaggttttaccgataacgaagtgacgctggtcatcgacgggcgctcgcatatcctttctgtgacggcccagcgtatgccggacggcatgatcctgctggagatggctccgatggataaccagcgccgcttaagtcaggaacagctacagcacgcccagcaggttactgcccgtgatttagtgcgcggcctggcacatgagattaaaaatccgcttggcggtttacgtggcgcggcgcagctgctcagcaaagcgttacctgacccatcactactcgaatataccaaagtgattatcgaacaggcggaccggctgcgaaatctggtcgaccgtctgttggggccgcagctgcccggtacgcgcgttaccgaaagtattcacaaagtggctgaacgcgtggtaacgctggtgtcgatggaactgccggacaacgtgcggttgattcgtgattacgatcccagcctaccggaactggcgcacgacccggatcaaattgaacaggtcttgctgaatattgtgcgcaatgcgctacaggcgctggggccggaaggcggtgaaatcattctgcgtacccgcaccgcgtttcaactgaccttacacggcgagcgctaccggctggcggcgcggattgatgtggaagataacgggccgggcattccgcctcatttgcaggatacgctgttttacccgatggtcagcggccgcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatacactggaaacatcaaggtcg
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K2066518_sequence
1
atggtgagcaagggcgaagaagataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaataa
BBa_K2066126_sequence
1
ggtaggccggagcaggtgagtcgctctccaacgtgaagtttgtcagctatctgtagcccatctctgc
BBa_K2066546_sequence
1
cgtttatgcaacgagggatagtctgggtagtcgatgacgatagttccatccgttgggtgcttgaacgtgcgctcgctggggcaggtttaacctgtacgacgtttgagaacggcgcagaagtgctggaggcgctggcgagcaaaacgccggatgtgctgctttcagatatccgtatgccgggaatggacgggctggcgctgctcaagcagattaaacagcgccatccaatgcttccggtcatcattatgaccgcacattccgatctggatgctgccgtcagcgcctatcaacaaggggcgtttgattatctgcccaaaccgtttgatatcgacgaagcagtggcgctggttgagcgcgctatcagtcattaccaggaacagcagcagccgcgtaatgttcagcttaacggcccaacgaccgatatcatcggcgaagcgccagccatgcaggacgtgttccgtattatcggtcggctttcgcgttcttctattagcgtgctgattaacggcgaatccggcaccggtaaagaactggtcgctcatgccctgcatcgccacagtccgcgcgccaaagcgccgtttatcgcgctgaatatggcagctatcccaaaagatttgatcgaatcagaactgtttggccacgagaaaggcgcgtttactggcgcgaataccattcgtcaggggcgttttgaacaggccgatggcggtacattattcctcgacgaaattggtgatatgccgctggatgtgcagacgcgtttgctgcgcgtgctggcagacggtcagttttaccgcgttggcggctatgcgccggtgaaagtggatgtgcggattatcgctgccactcaccagaatctcgaacagcgagtgcaggaaggtaagttccgtgaggatctgttccaccgcctgaacgttatccgcgttcatctgccgccgctgcgcgaacgtcgggaagatattccccgtctggcgcgccattttttacaggttgccgcgcgcgaactgggcgtagaagcgaagttactgcatccggaaaccgaagctgctctgacgcgtctggcgtggccaggcaacgtgcgccagctggaaaacacctgccgctggctaacggtgatggccgccgggcaggaagtgttgattcaggatttgcccggcgaactgtttgaatcaacggttgcggagagtacttcgcaaatgcaaccggacagctgggcgacgcttcttgcgcagtgggcagacagagcgctgcgttccggtcatcaaaatctgctttccgaagcgcagccagagctggagcggacgttactgacgaccgcgttgcgacatacgcaggggcataaacaggaagcggcgcggctactcggctggggccgcaacaccctgacgcgtaagttaaaagagctggggatggagtgattcacagcttgtgtgtaaagattgattattgagcgcaaattgctggtattttacgctttactgttccaggaggaaaaaa
BBa_I739101_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagtccctatcagtgatagagattccctatcagtgatagagat
BBa_K2066538_sequence
1
atggcaacaggcacgcagcccgatgctgggcagatcctcaactcgctgattaacagtattttgttaatcgatgacaacctggcgatccattacgccaaccctgccgcgcaacaactgctcgcccaaagctcccgcaaattgtttggtacaccgttaccggaactgttgagctacttctcattaaatatcgagctgatgcaagaaagtctggaggcggggcaaggttttaccgataacgaagtgacgctggtcatcgacgggcgctcgcatatcctttctgtgacggcccagcgtatgccggacggcatgatcctgctggagatggctccgatggataaccagcgccgcttaagtcaggaacagctacagcacgcccagcaggttactgcccgtgatttagtgcgcggcctggcacatgagattaaaaatccgcttggcggtttacgtggcgcggcgcagctgctcagcaaagcgttacctgacccatcactactcgaatataccaaagtgattatcgaacaggcggaccggctgcgaaatctggtcgaccgtctgttggggccgcagctgcccggtacgcgcgttaccgaaagtattcacaaagtggctgaacgcgtggtaacgctggtgtcgatggaactgccggacaacgtgcggttgattcgtgattacgatcccagcctaccggaactggcgcacgacccggatcaaattgaacaggtcttgctgaatattgtgcgcaatgcgctacaggcgctggggccggaaggcggtgaaatcattctgcgtacccgcaccgcgtttcaactgaccttacacggcgagcgctaccggctggcggcgcggattgatgtggaagataacgggccgggcattccgcctcatttgcaggatacgctgttttacccgatggtcagcggccgcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K2066547_sequence
1
tcacaattaagcttggcgaaggtggcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagggcataccgagttctcggtttacctgcctatcaggaaataa
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z