Synthetic Enhancer Project: UNS 55as NRII TetR
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, ￼Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
Double Promoter (constitutive / TetR, negative)
Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss
Double terminator consisting of BBa_B0010 and BBa_B0012
Modified from the mCherry used in K2066028 and K2066029 to include the start codon. This modified mCherry is used in the construction of K2066040 and K2066041.
The start codon was removed in K2066028 and K2066029 to follow the design from Synthetic Analog Computation in Living Cells (Daniel et al.) where the codon bias was being optimized.
Synthetic Enhancer Project: NRII2302 Upstream Regulatory Region with Promoter
Synthetic Enhancer Project: 2X TetO Binding Cassette (55AS)
Synthetic Enhancer Project: NRII2302 Mutant Coding
Synthetic Enhancer Project: Enhancer/NRI binding sites
Synthetic Enhancer Project: glnAp2 promoter
Synthetic Enhancer Project: LacI binding site (LacO)
Synthetic Enhancer Project: Spacer between Promoter and RBS
Transcriptional terminator consisting of a 64 bp stem-loop.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Conversion of the iGEM parts registry to SBOL2.1