##gff-version 3
##sequence-region BBa_K2066124 1 4138
BBa_K2066124	.	sequence_feature	350	371	.	+	0	ID=BBa_K2066545;Name=BBa_K2066545
BBa_K2066124	.	sequence_feature	4119	4138	.	+	0	ID=BBa_K2066540;Name=BBa_K2066540
BBa_K2066124	.	sequence_feature	124	170	.	+	0	ID=BBa_K2066543;Name=BBa_K2066543
BBa_K2066124	.	sequence_feature	1	20	.	+	0	ID=BBa_K2066541;Name=BBa_K2066541
BBa_K2066124	.	CDS	516	2009	.	+	0	ID=BBa_K2066546;Name=BBa_K2066546
BBa_K2066124	.	terminator	3990	4118	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2066124	.	terminator	3990	4069	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2066124	.	stem_loop	4001	4044	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2066124	.	engineered_region	3990	4069	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2066124	.	terminator	4078	4118	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2066124	.	engineered_region	4078	4118	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2066124	.	stop_codon	4111	4111	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2066124	.	polyA_site	4105	4118	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2066124	.	stem_loop	4085	4104	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2066124	.	engineered_region	2010	2723	.	+	0	ID=BBa_K2066518;Name=BBa_K2066518
BBa_K2066124	.	sequence_feature	2853	2872	.	+	0	ID=BBa_K2066539;Name=BBa_K2066539
BBa_K2066124	.	sequence_feature	104	123	.	+	0	ID=BBa_K2066542;Name=BBa_K2066542
BBa_K2066124	.	promoter	320	349	.	+	0	ID=BBa_K2066544;Name=BBa_K2066544
BBa_K2066124	.	sequence_feature	372	514	.	+	0	ID=BBa_K2066547;Name=BBa_K2066547
BBa_K2066124	.	terminator	2724	2852	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K2066124	.	terminator	2724	2803	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K2066124	.	stem_loop	2735	2778	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K2066124	.	engineered_region	2724	2803	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K2066124	.	terminator	2812	2852	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K2066124	.	engineered_region	2812	2852	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K2066124	.	stop_codon	2845	2845	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K2066124	.	polyA_site	2839	2852	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K2066124	.	stem_loop	2819	2838	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K2066124	.	sequence_feature	171	319	.	+	0	ID=BBa_K2066531;Name=BBa_K2066531
BBa_K2066124	.	promoter	2873	2939	.	+	0	ID=BBa_K2066126;Name=BBa_K2066126
BBa_K2066124	.	engineered_region	2873	2912	.	+	0	ID=annotation2500695;Name=BBa_K2066018;Parent=BBa_K2066126
BBa_K2066124	.	promoter	21	103	.	+	0	ID=BBa_I739101;Name=BBa_I739101
BBa_K2066124	.	sequence_feature	56	63	.	+	0	ID=annotation1946099;Name=Scar;Parent=BBa_I739101
BBa_K2066124	.	non_covalent_binding_site	64	83	.	+	0	ID=annotation1946102;Name=TetR 1;Parent=BBa_I739101
BBa_K2066124	.	promoter	21	55	.	+	0	ID=annotation1946100;Name=J23100;Parent=BBa_I739101
BBa_K2066124	.	operator	64	103	.	+	0	ID=annotation1946257;Name=R0040 (2x TetR 1);Parent=BBa_I739101
BBa_K2066124	.	non_covalent_binding_site	84	103	.	+	0	ID=annotation1946101;Name=TetR 1;Parent=BBa_I739101
BBa_K2066124	.	CDS	2940	3989	.	+	0	ID=BBa_K2066538;Name=BBa_K2066538
>BBa_K2066124
gctgggagttcgtagacggattgacggctagctcagtcctaggtacagtgctagctactagagtccctatcagtgatag
agattccctatcagtgatagagataacaaacgcagaatccaagctcacaattgcaccaacatggtgcttaatgtttcca
ttgaagcactatattggtgcaacattgtcttcgacgctagctctctatcactgatagggatgtcggaggctcgcggact
ctatcaatgatagagtctcgagtctggccggatctctatcactgatagggagctagcgtccccatgataacgcctttta
ggggcaatttaaaagttggcacagatttcgctttatcttttttaattgtgagcgctcacaattaagcttggcgaaggtg
gcaccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccagg
gcataccgagttctcggtttacctgcctatcaggaaataaacgtttatgcaacgagggatagtctgggtagtcgatgac
gatagttccatccgttgggtgcttgaacgtgcgctcgctggggcaggtttaacctgtacgacgtttgagaacggcgcag
aagtgctggaggcgctggcgagcaaaacgccggatgtgctgctttcagatatccgtatgccgggaatggacgggctggc
gctgctcaagcagattaaacagcgccatccaatgcttccggtcatcattatgaccgcacattccgatctggatgctgcc
gtcagcgcctatcaacaaggggcgtttgattatctgcccaaaccgtttgatatcgacgaagcagtggcgctggttgagc
gcgctatcagtcattaccaggaacagcagcagccgcgtaatgttcagcttaacggcccaacgaccgatatcatcggcga
agcgccagccatgcaggacgtgttccgtattatcggtcggctttcgcgttcttctattagcgtgctgattaacggcgaa
tccggcaccggtaaagaactggtcgctcatgccctgcatcgccacagtccgcgcgccaaagcgccgtttatcgcgctga
atatggcagctatcccaaaagatttgatcgaatcagaactgtttggccacgagaaaggcgcgtttactggcgcgaatac
cattcgtcaggggcgttttgaacaggccgatggcggtacattattcctcgacgaaattggtgatatgccgctggatgtg
cagacgcgtttgctgcgcgtgctggcagacggtcagttttaccgcgttggcggctatgcgccggtgaaagtggatgtgc
ggattatcgctgccactcaccagaatctcgaacagcgagtgcaggaaggtaagttccgtgaggatctgttccaccgcct
gaacgttatccgcgttcatctgccgccgctgcgcgaacgtcgggaagatattccccgtctggcgcgccattttttacag
gttgccgcgcgcgaactgggcgtagaagcgaagttactgcatccggaaaccgaagctgctctgacgcgtctggcgtggc
caggcaacgtgcgccagctggaaaacacctgccgctggctaacggtgatggccgccgggcaggaagtgttgattcagga
tttgcccggcgaactgtttgaatcaacggttgcggagagtacttcgcaaatgcaaccggacagctgggcgacgcttctt
gcgcagtgggcagacagagcgctgcgttccggtcatcaaaatctgctttccgaagcgcagccagagctggagcggacgt
tactgacgaccgcgttgcgacatacgcaggggcataaacaggaagcggcgcggctactcggctggggccgcaacaccct
gacgcgtaagttaaaagagctggggatggagtgattcacagcttgtgtgtaaagattgattattgagcgcaaattgctg
gtattttacgctttactgttccaggaggaaaaaaatggtgagcaagggcgaagaagataacatggccatcatcaaggag
ttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcc
cctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccc
tcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgag
ggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccttgcaggacg
gcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatggg
ctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaag
gacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacg
tcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgcca
ctccaccggcggcatggacgagctgtacaagtaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatagcactgaaggtcctcaatcgggtaggccggagcaggtgagtcgctctccaacgtgaagtttgtcagctatc
tgtagcccatctctgcatggcaacaggcacgcagcccgatgctgggcagatcctcaactcgctgattaacagtattttg
ttaatcgatgacaacctggcgatccattacgccaaccctgccgcgcaacaactgctcgcccaaagctcccgcaaattgt
ttggtacaccgttaccggaactgttgagctacttctcattaaatatcgagctgatgcaagaaagtctggaggcggggca
aggttttaccgataacgaagtgacgctggtcatcgacgggcgctcgcatatcctttctgtgacggcccagcgtatgccg
gacggcatgatcctgctggagatggctccgatggataaccagcgccgcttaagtcaggaacagctacagcacgcccagc
aggttactgcccgtgatttagtgcgcggcctggcacatgagattaaaaatccgcttggcggtttacgtggcgcggcgca
gctgctcagcaaagcgttacctgacccatcactactcgaatataccaaagtgattatcgaacaggcggaccggctgcga
aatctggtcgaccgtctgttggggccgcagctgcccggtacgcgcgttaccgaaagtattcacaaagtggctgaacgcg
tggtaacgctggtgtcgatggaactgccggacaacgtgcggttgattcgtgattacgatcccagcctaccggaactggc
gcacgacccggatcaaattgaacaggtcttgctgaatattgtgcgcaatgcgctacaggcgctggggccggaaggcggt
gaaatcattctgcgtacccgcaccgcgtttcaactgaccttacacggcgagcgctaccggctggcggcgcggattgatg
tggaagataacgggccgggcattccgcctcatttgcaggatacgctgttttacccgatggtcagcggccgcgaaggtgg
caccgggcttggcttatccatcgctcgtaatttgattgatcagcattcaggcaaaattgaatttaccagttggccaggg
cataccgagttctcggtttacctgcctatcaggaaataaccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttatacactggaaacatcaaggtcg