LOCUS       BBa_K2068000            1406 bp    DNA     linear       05-Nov-2024
DEFINITION  PCQuad 3.0 Wildtype
ACCESSION   BBa_K2068000
VERSION     BBa_K2068000.1  
FEATURES             Location/Qualifiers
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     misc_feature    39..1400
                     /label=PCQuad 3.0
     misc_feature    1382..1400
                     /label=6X His Tag
     misc_feature    1401..1405
                     /label=Stop Codon 
     misc_feature    33..38
                     /label=Scar
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gccgttcatt accgtaggac
       61 aagaaaattc tacgtctatt gatttgtatt atgaggacca tggtaccggt acgccggtgg
      121 ttctcatcca cggctttccg ctatcaggac attcttggga gcgtcagagc gctgcgcttt
      181 tagatgccgg tgctcgtgta ataacgtacg atagacgcgg ttttggccag agctctcagc
      241 caacgactgg atacgattat gacaccttcg ccgccgattt aaatactgtt ctggaaaccc
      301 tggatcttca ggatgcggtc ttagttggtt ttagtatggg cacaggtgaa gttgcccgct
      361 acgtcagttc ttatggcact gctcgtattg cagcggtagc ttttctggct agcttagaac
      421 cctttctatt aaaaaccgat gataatccgg atggggcggc tccacaggag tttttcgacg
      481 gaattgtggc cgctgtgaaa gccgatagat atgctttcta tactgggttt ttcaatgact
      541 tctataattt agatgaaaac ctgggaacac gcatctcgga agaggctgta cggaactcat
      601 ggaatactgc ggcatctggc ggattctttg ctgccgcagc cgcgccgacc acttggtata
      661 cagattttcg tgcggacatt cctagaattg atgtacctgc cctgattctg cacggtacgg
      721 gcgaccgtac cttaccgatt gagaacactg cccgcgtctt tcataaagct cttccgtccg
      781 ctgagtacgt agaggttgaa ggggcacctc atggtctgtt atggactcat gctgaagaag
      841 tcaacacagc cctgcttgct ttccttgcga aggctcaaga agcccaaaag cagaaattac
      901 tgaccgaagt ggaaacttat gtactatcca tcataccgtc tggtcccctt aaagcagaaa
      961 tcgcacaacg tctggaggat gtcttcgcgg gcaaaaatac agacctggag gtgctgatgg
     1021 aatggcttaa gacccgaccg attctgtcac cgttaacgaa gggtatccta ggattcgttt
     1081 ttaccctgac ggtgcccagt gagcgcggac tgcaacgaag aagattcgtc caaaacgcat
     1141 taaacgggaa tggtgaccca aacaatatgg acaaagcggt gaagctgtat cgaaaactta
     1201 agcgcgaaat aacattccat ggggccaaag aaattagcct gagctattcc gctggggcgc
     1261 ttgcttcttg tatgggttta atatataacc gaatgggtgc ggtcaccacg gaagtcgcgt
     1321 tcggattagt atgcgcgaca tgcgagcaga ttgcagactc tcagcataga agccatcgtc
     1381 agcatcacca ccatcaccat taataa
//