BBa_K2068000 1 BBa_K2068000 PCQuad 3.0 Wildtype 2016-09-06T11:00:00Z 2016-09-14T08:27:05Z This cage was designed by Dr. Todd Yeates at UCLA. It's subunits are a fusion of a naturally trimeric protein(Bromoperoxidase) and a naturally dimeric protein(M1 Matrix). The PCQuad gene codes for the production of one subunit of a protein cage. Each subunit is made up of a dimer protein(M1 Matrix) and a trimer protein(Bromoperoxidase) fused by an alpha helical linker. Expression of this subunit will lead to the self assembly of a tetrahedral structured protein cage. Protein cages, are self assembling supramolecular protein structures where multiple protein subunits interact non covalently to form a higher order structure. Our protein cage, PCquad, is a 12 subunit cage where each subunit is a fusion of two naturally oligomerizing proteins -- a trimer(Bromoperoxidase) and a dimer(M1 Matrix). The alpha helical fusion protein that links the trimer and dimer fixes them in a very specific orientation allowing for self assembly once subunits interact with each other. false false _2536_ 27134 27134 9 false The fusion protein that links the dimer and trimer forms a 109.5 degree angle that makes self assembly more optimal. false Nithin Dharmaraj annotation2482646 1 Scar range2482646 1 33 38 annotation2482644 1 6X His Tag range2482644 1 1382 1400 annotation2482643 1 PCQuad 3.0 range2482643 1 39 1400 annotation2482645 1 Stop Codon range2482645 1 1401 1405 annotation2482641 1 T7 promoter range2482641 1 1 20 annotation2482642 1 RBS range2482642 1 24 27 BBa_K2068000_sequence 1 taatacgactcactatagggaaagaggagaaatactagatgccgttcattaccgtaggacaagaaaattctacgtctattgatttgtattatgaggaccatggtaccggtacgccggtggttctcatccacggctttccgctatcaggacattcttgggagcgtcagagcgctgcgcttttagatgccggtgctcgtgtaataacgtacgatagacgcggttttggccagagctctcagccaacgactggatacgattatgacaccttcgccgccgatttaaatactgttctggaaaccctggatcttcaggatgcggtcttagttggttttagtatgggcacaggtgaagttgcccgctacgtcagttcttatggcactgctcgtattgcagcggtagcttttctggctagcttagaaccctttctattaaaaaccgatgataatccggatggggcggctccacaggagtttttcgacggaattgtggccgctgtgaaagccgatagatatgctttctatactgggtttttcaatgacttctataatttagatgaaaacctgggaacacgcatctcggaagaggctgtacggaactcatggaatactgcggcatctggcggattctttgctgccgcagccgcgccgaccacttggtatacagattttcgtgcggacattcctagaattgatgtacctgccctgattctgcacggtacgggcgaccgtaccttaccgattgagaacactgcccgcgtctttcataaagctcttccgtccgctgagtacgtagaggttgaaggggcacctcatggtctgttatggactcatgctgaagaagtcaacacagccctgcttgctttccttgcgaaggctcaagaagcccaaaagcagaaattactgaccgaagtggaaacttatgtactatccatcataccgtctggtccccttaaagcagaaatcgcacaacgtctggaggatgtcttcgcgggcaaaaatacagacctggaggtgctgatggaatggcttaagacccgaccgattctgtcaccgttaacgaagggtatcctaggattcgtttttaccctgacggtgcccagtgagcgcggactgcaacgaagaagattcgtccaaaacgcattaaacgggaatggtgacccaaacaatatggacaaagcggtgaagctgtatcgaaaacttaagcgcgaaataacattccatggggccaaagaaattagcctgagctattccgctggggcgcttgcttcttgtatgggtttaatatataaccgaatgggtgcggtcaccacggaagtcgcgttcggattagtatgcgcgacatgcgagcagattgcagactctcagcatagaagccatcgtcagcatcaccaccatcaccattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z