BBa_K2069000 1 BBa_K2069000 RBS + Cpn60 + RBS + CspC 2016-10-11T11:00:00Z 2016-10-12T10:20:29Z This was assembled with parts from the 2016 distribution kit This intermediate part contains the assemble of Amsterdam's 2011 biobricks for their project icE. coli BBa_K538101 and BBa_K538104 false false _2537_ 29482 29482 9 false There were no special issues for the design of this part false Mara Valverde Rasc??n component2494103 1 BBa_K538101 component2494112 1 BBa_K538104 annotation2494112 1 BBa_K538104 range2494112 1 1677 1889 annotation2494103 1 BBa_K538101 range2494103 1 1 1668 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K538101 1 BBa_K538101 RBS + Cpn60 2011-09-07T11:00:00Z 2015-05-08T01:12:37Z - Released HQ 2013 - false false _704_ 0 9985 9 In stock false - false Bas Stringer component2133885 1 BBa_K538001 component2133881 1 BBa_B0034 annotation2133881 1 BBa_B0034 range2133881 1 1 12 annotation2133885 1 BBa_K538001 range2133885 1 19 1668 BBa_K538104 1 BBa_K538104 RBS + CspC 2011-09-07T11:00:00Z 2015-05-08T01:12:37Z - Released HQ 2013 - false false _704_ 0 9985 9 In stock false - false Bas Stringer component2133910 1 BBa_K538004 component2133904 1 BBa_B0034 annotation2133910 1 BBa_K538004 range2133910 1 19 213 annotation2133904 1 BBa_B0034 range2133904 1 1 12 BBa_K1364002 1 GAFP-1 RBS - Antifungal GAFP-1 2014-09-30T11:00:00Z 2015-06-08T08:39:49Z This gene was ordered from a synthesis company. The Gastrodia anti-fungal protein (GAFP-1) is a mannose-binding lectin which is able to inhibit the growth of multiples species of plant pathogenic fungi. The RBS used is a strong RBS for Bacillus subtilis (K780002). false false _1740_ 4206 21466 9 In stock false This part is composed of a Strong RBS (K780002) and the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program. false Manon Molina annotation2391443 1 GAFP-1 range2391443 1 140 187 annotation2391442 1 Propeptide range2391442 1 98 139 annotation2391440 1 strong RBS K780002 range2391440 1 1 16 annotation2391441 1 Signal peptide range2391441 1 17 97 BBa_K538001 1 Cpn60 Cpn60 (O. antarctica) 2011-07-28T11:00:00Z 2015-05-08T01:12:36Z De novo synthesis - false false _704_ 0 9985 9 Not in stock false - false Bas Stringer (Sequence by Paul van Dieken) annotation2124231 1 Start Codon range2124231 1 1 3 annotation2124232 1 Double Stop Codon range2124232 1 1645 1650 annotation2128866 1 A > G (Recode for SapI site) range2128866 1 908 908 BBa_K538004 1 CspC CspC (P. irgensii) 2011-09-07T11:00:00Z 2015-05-08T01:12:37Z - Released HQ 2013 - false false _704_ 0 9985 9 In stock true - false Bas Stringer (Sequence by Paul van Dieken) annotation2129606 1 RNA binding motif (RPN2) range2129606 1 79 93 annotation2127783 1 Start Codon range2127783 1 1 3 annotation2127785 1 A > G (Recode for SapI site) range2127785 1 75 75 annotation2127784 1 Double Stop Codon range2127784 1 190 195 annotation2129605 1 RNA binding motif (RPN1) range2129605 1 37 54 BBa_K2069002 1 BBa_K2069002 RBS + Cpn60 + RBS + CspC + RBS + Antifungal GAFP-1 + double terminator 2016-10-11T11:00:00Z 2016-10-12T11:14:51Z From the parts in the 2016 distribution kit This intermediate part allows the transcription of chaperonin 60, CspC and the GAFP-1 protein if added a promoter. This part has a double terminator (T1 E. coli and phage T7 early terminator) true false _2537_ 29482 29482 9 false No special issues in the design false Mara Valverde Rasc??n component2494191 1 BBa_K2069000 component2494196 1 BBa_K1364002 annotation2494191 1 BBa_K2069000 range2494191 1 1 1889 annotation2494196 1 BBa_K1364002 range2494196 1 1898 2084 BBa_K538001_sequence 1 atggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataa BBa_K538004_sequence 1 atgaataaaggtaccgtaaaatttttcaatgaatctaaaggattcggatttatcactgaagaaggaaacaatgaggagcattttgtgcacgtgtcaggattagtagacgaaattcgtgaaaacgatgaggttgaatttgacttacaagatggaagaaaaggattaaacgcagtaaacgtaagagttatataataa BBa_K2069000_sequence 1 aaagaggagaaatactagatggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataatactagagaaagaggagaaatactagatgaataaaggtaccgtaaaatttttcaatgaatctaaaggattcggatttatcactgaagaaggaaacaatgaggagcattttgtgcacgtgtcaggattagtagacgaaattcgtgaaaacgatgaggttgaatttgacttacaagatggaagaaaaggattaaacgcagtaaacgtaagagttatataataa BBa_B0034_sequence 1 aaagaggagaaa BBa_K538101_sequence 1 aaagaggagaaatactagatggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataa BBa_K1364002_sequence 1 agagaacaaggaggggatgtttgcaaagagatttaagacgagtttacttccgctgtttgccggcttccttttgctgtttcaccttgtattagccggtcctgcggcagcaagtgcggaaacggctaacaaaagtaacgagctggatagcctttccttttcatataataatttcgaagaggacgattaa BBa_K538104_sequence 1 aaagaggagaaatactagatgaataaaggtaccgtaaaatttttcaatgaatctaaaggattcggatttatcactgaagaaggaaacaatgaggagcattttgtgcacgtgtcaggattagtagacgaaattcgtgaaaacgatgaggttgaatttgacttacaagatggaagaaaaggattaaacgcagtaaacgtaagagttatataataa BBa_K2069002_sequence 1 aaagaggagaaatactagatggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataatactagagaaagaggagaaatactagatgaataaaggtaccgtaaaatttttcaatgaatctaaaggattcggatttatcactgaagaaggaaacaatgaggagcattttgtgcacgtgtcaggattagtagacgaaattcgtgaaaacgatgaggttgaatttgacttacaagatggaagaaaaggattaaacgcagtaaacgtaagagttatataataatactagagagagaacaaggaggggatgtttgcaaagagatttaagacgagtttacttccgctgtttgccggcttccttttgctgtttcaccttgtattagccggtcctgcggcagcaagtgcggaaacggctaacaaaagtaacgagctggatagcctttccttttcatataataatttcgaagaggacgattaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z