BBa_K2069002
type: ComponentDefinition
persistent identity: BBa K2069002
display id: BBa_K2069002
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + Cpn60 + RBS + CspC + RBS + Antifungal GAFP-1 + double terminator
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T11:14:51Z
mutable provenance: From the parts in the 2016 distribution kit
owned by: james
owned by: myers
mutable description: This intermediate part allows the transcription of chaperonin 60, CspC and the GAFP-1 protein if added a promoter. This part has a double terminator (T1 E. coli and phage T7 early terminator)
discontinued: true
dominant: false
experience: experience/None
group u list: _2537_
m user id: 29482
owner id: 29482
owning group id: 9
status: status/Deleted
bookmark: false
mutable notes: No special issues in the design
star: false
creator: Mara Valverde Rasc??n
type: DnaRegion
role: SO:0000804
role: partType/Intermediate
component: BBa_K2069000
component: BBa_K1364002
sequence annotation: BBa_K2069000
sequence annotation: BBa_K1364002
sequence: https://synbiohub.org/public/igem/BBa_K2069002_sequence/1

BBa_K2069000
type: Component
persistent identity: component2494191
display id: component2494191
version: 1
top level: BBa_K2069002
definition: BBa_K2069000
access: public

BBa_K1364002
type: Component
persistent identity: component2494196
display id: component2494196
version: 1
top level: BBa_K2069002
definition: GAFP-1
access: public

BBa_K2069000
type: SequenceAnnotation
persistent identity: annotation2494191
display id: annotation2494191
version: 1
top level: BBa_K2069002
location: https://synbiohub.org/public/igem/BBa_K2069002/annotation2494191/range2494191/1
component: BBa_K2069000

https://synbiohub.org/public/igem/BBa_K2069002/annotation2494191/range2494191/1
type: Range
persistent identity: range2494191
display id: range2494191
version: 1
top level: BBa_K2069002
direction: direction/reverse
start: 1
end: 1889
orientation: inline

BBa_K1364002
type: SequenceAnnotation
persistent identity: annotation2494196
display id: annotation2494196
version: 1
top level: BBa_K2069002
location: https://synbiohub.org/public/igem/BBa_K2069002/annotation2494196/range2494196/1
component: BBa_K1364002

https://synbiohub.org/public/igem/BBa_K2069002/annotation2494196/range2494196/1
type: Range
persistent identity: range2494196
display id: range2494196
version: 1
top level: BBa_K2069002
direction: direction/reverse
start: 1898
end: 2084
orientation: inline

GAFP-1
type: ComponentDefinition
persistent identity: BBa K1364002
display id: BBa_K1364002
version: 1
was generated by: iGEM to SBOL conversion
description: RBS - Antifungal GAFP-1
created: 2014-09-30T11:00:00Z
modified: 2015-06-08T08:39:49Z
mutable provenance: This gene was ordered from a synthesis company.
owned by: james
owned by: myers
mutable description: The Gastrodia anti-fungal protein (GAFP-1) is a mannose-binding lectin which is able to inhibit the growth of multiples species of plant pathogenic fungi. The RBS used is a strong RBS for Bacillus subtilis (K780002).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1740_
m user id: 4206
owner id: 21466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part is composed of a Strong RBS (K780002) and the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program.
star: false
creator: Manon Molina
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: strong RBS K780002
sequence annotation: Signal peptide
sequence annotation: Propeptide
sequence annotation: GAFP-1
sequence: https://synbiohub.org/public/igem/BBa_K1364002_sequence/1

strong RBS K780002
type: SequenceAnnotation
persistent identity: annotation2391440
display id: annotation2391440
version: 1
top level: GAFP-1
location: https://synbiohub.org/public/igem/BBa_K1364002/annotation2391440/range2391440/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K1364002/annotation2391440/range2391440/1
type: Range
persistent identity: range2391440
display id: range2391440
version: 1
top level: GAFP-1
direction: direction/forward
start: 1
end: 16
orientation: inline

Signal peptide
type: SequenceAnnotation
persistent identity: annotation2391441
display id: annotation2391441
version: 1
top level: GAFP-1
location: https://synbiohub.org/public/igem/BBa_K1364002/annotation2391441/range2391441/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1364002/annotation2391441/range2391441/1
type: Range
persistent identity: range2391441
display id: range2391441
version: 1
top level: GAFP-1
direction: direction/forward
start: 17
end: 97
orientation: inline

Propeptide
type: SequenceAnnotation
persistent identity: annotation2391442
display id: annotation2391442
version: 1
top level: GAFP-1
location: https://synbiohub.org/public/igem/BBa_K1364002/annotation2391442/range2391442/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1364002/annotation2391442/range2391442/1
type: Range
persistent identity: range2391442
display id: range2391442
version: 1
top level: GAFP-1
direction: direction/forward
start: 98
end: 139
orientation: inline

GAFP-1
type: SequenceAnnotation
persistent identity: annotation2391443
display id: annotation2391443
version: 1
top level: GAFP-1
location: https://synbiohub.org/public/igem/BBa_K1364002/annotation2391443/range2391443/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K1364002/annotation2391443/range2391443/1
type: Range
persistent identity: range2391443
display id: range2391443
version: 1
top level: GAFP-1
direction: direction/forward
start: 140
end: 187
orientation: inline

BBa_K2069000
type: ComponentDefinition
persistent identity: BBa K2069000
display id: BBa_K2069000
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + Cpn60 + RBS + CspC
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T10:20:29Z
mutable provenance: This was assembled with parts from the 2016 distribution kit
owned by: james
owned by: myers
mutable description: This intermediate part contains the assemble of Amsterdam's 2011 biobricks for their project icE. coli BBa_K538101 and BBa_K538104
discontinued: false
dominant: false
group u list: _2537_
m user id: 29482
owner id: 29482
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: There were no special issues for the design of this part
star: false
creator: Mara Valverde Rasc??n
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K538104
component: BBa_K538101
sequence annotation: BBa_K538101
sequence annotation: BBa_K538104
sequence: https://synbiohub.org/public/igem/BBa_K2069000_sequence/1

BBa_K538104
type: Component
persistent identity: component2494112
display id: component2494112
version: 1
top level: BBa_K2069000
definition: BBa_K538104
access: public

BBa_K538101
type: Component
persistent identity: component2494103
display id: component2494103
version: 1
top level: BBa_K2069000
definition: BBa_K538101
access: public

BBa_K538101
type: SequenceAnnotation
persistent identity: annotation2494103
display id: annotation2494103
version: 1
top level: BBa_K2069000
location: https://synbiohub.org/public/igem/BBa_K2069000/annotation2494103/range2494103/1
component: BBa_K538101

https://synbiohub.org/public/igem/BBa_K2069000/annotation2494103/range2494103/1
type: Range
persistent identity: range2494103
display id: range2494103
version: 1
top level: BBa_K2069000
direction: direction/reverse
start: 1
end: 1668
orientation: inline

BBa_K538104
type: SequenceAnnotation
persistent identity: annotation2494112
display id: annotation2494112
version: 1
top level: BBa_K2069000
location: https://synbiohub.org/public/igem/BBa_K2069000/annotation2494112/range2494112/1
component: BBa_K538104

https://synbiohub.org/public/igem/BBa_K2069000/annotation2494112/range2494112/1
type: Range
persistent identity: range2494112
display id: range2494112
version: 1
top level: BBa_K2069000
direction: direction/reverse
start: 1677
end: 1889
orientation: inline

BBa_K538101
type: ComponentDefinition
persistent identity: BBa K538101
display id: BBa_K538101
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + Cpn60
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: -
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Bas Stringer
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_K538001
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K538001
sequence: https://synbiohub.org/public/igem/BBa_K538101_sequence/1

BBa_K538001
type: Component
persistent identity: component2133885
display id: component2133885
version: 1
top level: BBa_K538101
definition: Cpn60
access: public

BBa_B0034
type: Component
persistent identity: component2133881
display id: component2133881
version: 1
top level: BBa_K538101
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2133881
display id: annotation2133881
version: 1
top level: BBa_K538101
location: https://synbiohub.org/public/igem/BBa_K538101/annotation2133881/range2133881/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538101/annotation2133881/range2133881/1
type: Range
persistent identity: range2133881
display id: range2133881
version: 1
top level: BBa_K538101
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K538001
type: SequenceAnnotation
persistent identity: annotation2133885
display id: annotation2133885
version: 1
top level: BBa_K538101
location: https://synbiohub.org/public/igem/BBa_K538101/annotation2133885/range2133885/1
component: BBa_K538001

https://synbiohub.org/public/igem/BBa_K538101/annotation2133885/range2133885/1
type: Range
persistent identity: range2133885
display id: range2133885
version: 1
top level: BBa_K538101
direction: direction/reverse
start: 19
end: 1668
orientation: inline

BBa_K538104
type: ComponentDefinition
persistent identity: BBa K538104
display id: BBa_K538104
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + CspC
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: -
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -
discontinued: false
dominant: false
experience: experience/Works
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Bas Stringer
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_K538004
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K538004
sequence: https://synbiohub.org/public/igem/BBa_K538104_sequence/1

BBa_K538004
type: Component
persistent identity: component2133910
display id: component2133910
version: 1
top level: BBa_K538104
definition: CspC
access: public

BBa_B0034
type: Component
persistent identity: component2133904
display id: component2133904
version: 1
top level: BBa_K538104
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2133904
display id: annotation2133904
version: 1
top level: BBa_K538104
location: https://synbiohub.org/public/igem/BBa_K538104/annotation2133904/range2133904/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538104/annotation2133904/range2133904/1
type: Range
persistent identity: range2133904
display id: range2133904
version: 1
top level: BBa_K538104
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K538004
type: SequenceAnnotation
persistent identity: annotation2133910
display id: annotation2133910
version: 1
top level: BBa_K538104
location: https://synbiohub.org/public/igem/BBa_K538104/annotation2133910/range2133910/1
component: BBa_K538004

https://synbiohub.org/public/igem/BBa_K538104/annotation2133910/range2133910/1
type: Range
persistent identity: range2133910
display id: range2133910
version: 1
top level: BBa_K538104
direction: direction/reverse
start: 19
end: 213
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

Cpn60
type: ComponentDefinition
persistent identity: BBa K538001
display id: BBa_K538001
version: 1
was generated by: iGEM to SBOL conversion
description: Cpn60 (O. antarctica)
created: 2011-07-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
mutable provenance: De novo synthesis
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Bas Stringer (Sequence by Paul van Dieken)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: A > G (Recode for SapI site)
sequence annotation: Double Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K538001_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2124231
display id: annotation2124231
version: 1
top level: Cpn60
location: https://synbiohub.org/public/igem/BBa_K538001/annotation2124231/range2124231/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K538001/annotation2124231/range2124231/1
type: Range
persistent identity: range2124231
display id: range2124231
version: 1
top level: Cpn60
direction: direction/forward
start: 1
end: 3
orientation: inline

A > G (Recode for SapI site)
type: SequenceAnnotation
persistent identity: annotation2128866
display id: annotation2128866
version: 1
top level: Cpn60
location: https://synbiohub.org/public/igem/BBa_K538001/annotation2128866/range2128866/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K538001/annotation2128866/range2128866/1
type: Range
persistent identity: range2128866
display id: range2128866
version: 1
top level: Cpn60
direction: direction/forward
start: 908
end: 908
orientation: inline

Double Stop Codon
type: SequenceAnnotation
persistent identity: annotation2124232
display id: annotation2124232
version: 1
top level: Cpn60
location: https://synbiohub.org/public/igem/BBa_K538001/annotation2124232/range2124232/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K538001/annotation2124232/range2124232/1
type: Range
persistent identity: range2124232
display id: range2124232
version: 1
top level: Cpn60
direction: direction/forward
start: 1645
end: 1650
orientation: inline

CspC
type: ComponentDefinition
persistent identity: BBa K538004
display id: BBa_K538004
version: 1
was generated by: iGEM to SBOL conversion
description: CspC (P. irgensii)
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: -
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -
discontinued: false
dominant: false
experience: experience/Works
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: -
star: false
creator: Bas Stringer (Sequence by Paul van Dieken)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: RNA binding motif (RPN1)
sequence annotation: A > G (Recode for SapI site)
sequence annotation: RNA binding motif (RPN2)
sequence annotation: Double Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K538004_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2127783
display id: annotation2127783
version: 1
top level: CspC
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2127783/range2127783/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K538004/annotation2127783/range2127783/1
type: Range
persistent identity: range2127783
display id: range2127783
version: 1
top level: CspC
direction: direction/forward
start: 1
end: 3
orientation: inline

RNA binding motif (RPN1)
type: SequenceAnnotation
persistent identity: annotation2129605
display id: annotation2129605
version: 1
top level: CspC
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2129605/range2129605/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K538004/annotation2129605/range2129605/1
type: Range
persistent identity: range2129605
display id: range2129605
version: 1
top level: CspC
direction: direction/forward
start: 37
end: 54
orientation: inline

A > G (Recode for SapI site)
type: SequenceAnnotation
persistent identity: annotation2127785
display id: annotation2127785
version: 1
top level: CspC
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2127785/range2127785/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K538004/annotation2127785/range2127785/1
type: Range
persistent identity: range2127785
display id: range2127785
version: 1
top level: CspC
direction: direction/forward
start: 75
end: 75
orientation: inline

RNA binding motif (RPN2)
type: SequenceAnnotation
persistent identity: annotation2129606
display id: annotation2129606
version: 1
top level: CspC
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2129606/range2129606/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K538004/annotation2129606/range2129606/1
type: Range
persistent identity: range2129606
display id: range2129606
version: 1
top level: CspC
direction: direction/forward
start: 79
end: 93
orientation: inline

Double Stop Codon
type: SequenceAnnotation
persistent identity: annotation2127784
display id: annotation2127784
version: 1
top level: CspC
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2127784/range2127784/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K538004/annotation2127784/range2127784/1
type: Range
persistent identity: range2127784
display id: range2127784
version: 1
top level: CspC
direction: direction/forward
start: 190
end: 195
orientation: inline

https://synbiohub.org/public/igem/BBa_K2069002_sequence/1
type: Sequence
persistent identity: BBa K2069002 sequence
display id: BBa_K2069002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataatactagagaaagaggagaaatactagatgaataaaggtaccgtaaaatttttcaatgaatctaaaggattcggatttatcactgaagaaggaaacaatgaggagcattttgtgcacgtgtcaggattagtagacgaaattcgtgaaaacgatgaggttgaatttgacttacaagatggaagaaaaggattaaacgcagtaaacgtaagagttatataataatactagagagagaacaaggaggggatgtttgcaaagagatttaagacgagtttacttccgctgtttgccggcttccttttgctgtttcaccttgtattagccggtcctgcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1364002_sequence/1
type: Sequence
persistent identity: BBa K1364002 sequence
display id: BBa_K1364002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaacaaggaggggatgtttgcaaagagatttaagacgagtttacttccgctgtttgccggcttccttttgctgtttcaccttgtattagccggtcctgcggcagcaagtgcggaaacggctaacaaaagtaacgagctggatagcctttccttttcatataataatttcgaagaggacgattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2069000_sequence/1
type: Sequence
persistent identity: BBa K2069000 sequence
display id: BBa_K2069000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataatactagagaaagaggagaaatactagatgaataaaggtaccgtaaaatttttcaatgaatctaaaggattcggatttatcactgaagaaggaaacaatgaggagcattttgtgcacgtgtcaggattagtagacgaaattcgtgaaaacgatgaggttgaatttgacttacaagatggaagaaaaggattaaacgcagtaaacgtaagagttatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K538101_sequence/1
type: Sequence
persistent identity: BBa K538101 sequence
display id: BBa_K538101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K538104_sequence/1
type: Sequence
persistent identity: BBa K538104 sequence
display id: BBa_K538104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaataaaggtaccgtaaaatttttcaatgaatctaaaggattcggatttatcactgaagaaggaaacaatgaggagcattttgtgcacgtgtcaggattagtagacgaaattcgtgaaaacgatgaggttgaatttgacttacaagatggaagaaaaggattaaacgcagtaaacgtaagagttatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K538001_sequence/1
type: Sequence
persistent identity: BBa K538001 sequence
display id: BBa_K538001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K538004_sequence/1
type: Sequence
persistent identity: BBa K538004 sequence
display id: BBa_K538004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaataaaggtaccgtaaaatttttcaatgaatctaaaggattcggatttatcactgaagaaggaaacaatgaggagcattttgtgcacgtgtcaggattagtagacgaaattcgtgaaaacgatgaggttgaatttgacttacaagatggaagaaaaggattaaacgcagtaaacgtaagagttatataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z