##gff-version 3
##sequence-region BBa_K2069004 1 3308
BBa_K2069004	.	engineered_region	1225	3308	.	+	0	ID=BBa_K2069002;Name=BBa_K2069002
BBa_K2069004	.	engineered_region	1225	3113	.	+	0	ID=BBa_K2069000;Name=BBa_K2069000;Parent=BBa_K2069002
BBa_K2069004	.	engineered_region	2901	3113	.	+	0	ID=BBa_K538104;Name=BBa_K538104;Parent=BBa_K2069000
BBa_K2069004	.	CDS	2919	3113	.	+	0	ID=BBa_K538004;Name=BBa_K538004;Parent=BBa_K538104
BBa_K2069004	.	point_mutation	2993	2993	.	+	0	ID=annotation2127785;Name=A > G (Recode for SapI site);Parent=BBa_K538004
BBa_K2069004	.	start_codon	2919	2921	.	+	0	ID=annotation2127783;Name=Start Codon;Parent=BBa_K538004
BBa_K2069004	.	non_covalent_binding_site	2955	2972	.	+	0	ID=annotation2129605;Name=RNA binding motif (RPN1);Parent=BBa_K538004
BBa_K2069004	.	stop_codon	3108	3113	.	+	0	ID=annotation2127784;Name=Double Stop Codon;Parent=BBa_K538004
BBa_K2069004	.	non_covalent_binding_site	2997	3011	.	+	0	ID=annotation2129606;Name=RNA binding motif (RPN2);Parent=BBa_K538004
BBa_K2069004	.	ribosome_entry_site	2901	2912	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K538104
BBa_K2069004	.	conserved_region	2905	2908	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2069004	.	engineered_region	1225	2892	.	+	0	ID=BBa_K538101;Name=BBa_K538101;Parent=BBa_K2069000
BBa_K2069004	.	ribosome_entry_site	1225	1236	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K538101
BBa_K2069004	.	conserved_region	1229	1232	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2069004	.	CDS	1243	2892	.	+	0	ID=BBa_K538001;Name=BBa_K538001;Parent=BBa_K538101
BBa_K2069004	.	point_mutation	2150	2150	.	+	0	ID=annotation2128866;Name=A > G (Recode for SapI site);Parent=BBa_K538001
BBa_K2069004	.	start_codon	1243	1245	.	+	0	ID=annotation2124231;Name=Start Codon;Parent=BBa_K538001
BBa_K2069004	.	stop_codon	2887	2892	.	+	0	ID=annotation2124232;Name=Double Stop Codon;Parent=BBa_K538001
BBa_K2069004	.	engineered_region	3122	3308	.	+	0	ID=BBa_K1364002;Name=BBa_K1364002;Parent=BBa_K2069002
BBa_K2069004	.	CDS	3219	3260	.	+	0	ID=annotation2391442;Name=Propeptide;Parent=BBa_K1364002
BBa_K2069004	.	ribosome_entry_site	3122	3137	.	+	0	ID=annotation2391440;Name=strong RBS K780002;Parent=BBa_K1364002
BBa_K2069004	.	CDS	3261	3308	.	+	0	ID=annotation2391443;Name=GAFP-1;Parent=BBa_K1364002
BBa_K2069004	.	CDS	3138	3218	.	+	0	ID=annotation2391441;Name=Signal peptide;Parent=BBa_K1364002
BBa_K2069004	.	engineered_region	1	1216	.	+	0	ID=BBa_K2069003;Name=BBa_K2069003
BBa_K2069004	.	engineered_region	902	1216	.	+	0	ID=BBa_K538100;Name=BBa_K538100;Parent=BBa_K2069003
BBa_K2069004	.	ribosome_entry_site	902	913	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K538100
BBa_K2069004	.	conserved_region	906	909	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K2069004	.	CDS	920	1216	.	+	0	ID=BBa_K538000;Name=BBa_K538000;Parent=BBa_K538100
BBa_K2069004	.	start_codon	920	922	.	+	0	ID=annotation2124174;Name=Start Codon;Parent=BBa_K538000
BBa_K2069004	.	stop_codon	1211	1216	.	+	0	ID=annotation2124175;Name=Double Stop Codon;Parent=BBa_K538000
BBa_K2069004	.	engineered_region	1	893	.	+	0	ID=BBa_K2069001;Name=BBa_K2069001;Parent=BBa_K2069003
BBa_K2069004	.	promoter	1	32	.	+	0	ID=BBa_K525998;Name=BBa_K525998;Parent=BBa_K2069001
BBa_K2069004	.	promoter	1	20	.	+	0	ID=annotation2129432;Name=T7 promoter;Parent=BBa_K525998
BBa_K2069004	.	engineered_region	21	32	.	+	0	ID=annotation2127788;Name=B0034;Parent=BBa_K525998
BBa_K2069004	.	ribosome_entry_site	24	27	.	+	0	ID=annotation2129433;Name=RBS;Parent=BBa_K525998
BBa_K2069004	.	engineered_region	39	893	.	+	0	ID=BBa_K541506;Name=BBa_K541506;Parent=BBa_K2069001
BBa_K2069004	.	CDS	39	893	.	+	0	ID=annotation2123461;Name=Reflectin;Parent=BBa_K541506
>BBa_K2069004
taatacgactcactatagggaaagaggagaaatactagatgaatcgctttatgaatcgctatcgcccgatgttcaataa
tatgtactctaacatgtaccgtggccgctaccgcggtatgatggaaccgatgagccgcatgactatggactttcagggc
cgctacatggacagccaaggccgcatggtggacccgcgctactacgattactacggccgcttcaacgattacgaccgct
actatggccgcagcatgttcaactatggctggatgatggacggcgatcgctacaaccgctataaccgctggatggatta
cccggagcgctacatggatatgtctggctaccagatggatatgtctggccgctggatggatatgcagggccgccactgc
aacccgtattctcagtggatgatgtacaactacaaccgccatggctactacccgaactactcttacggccgccacatgt
tttatccggaacgctggatggacatgtctaactactctatggacatgtatggccgctatatggatcgctggggccgcta
ctgtaacccgttttctcagtacatgaactactacggccgctactggaactacccgggctacaataattactactattct
cgcaacatgtattacccggaacgctactttgacatgtctaactggcagatggacatgcaaggccgctggatggacaacc
agggccgctattgttccccgtactggaacaactggtacggccgccatatgtactacccgtaccagaacaactacttcta
cggccgctatgactacccgggcatggactattccaactaccaaatggacatgcagggccgctacatggaccagtacggc
atgaacgactactactattaataatactagagaaagaggagaaatactagatgaaaatccgtccattacatgatcgtat
tgttgttcgccgtaaagaagaagagaccgcaactgcgggtggtattattttaccgggcgctgcggcagaaaaaccaaat
caaggtgttgttatctctgtgggtactggccgtattcttgataatggttcagtgcaagcgctggcggttaacgaaggcg
atgttgtcgtttttggtaaatactcaggtcaaaatactatcgatatcgatggtgaagaattattgattttgaatgaaag
tgatatctacggcgttttagaagcttaataatactagagaaagaggagaaatactagatggctgctaaagacgtattat
ttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaa
aggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatc
gaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtg
acggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatcc
aatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttg
gatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcga
tggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaagg
catgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatcca
ttaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtc
gtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacatt
caaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggt
ggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttg
ttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccg
tgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgtt
gccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaa
ctcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgt
tggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggt
aacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacag
gtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtc
tatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgat
atgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataatactagagaaagaggagaaatactagatgaa
taaaggtaccgtaaaatttttcaatgaatctaaaggattcggatttatcactgaagaaggaaacaatgaggagcatttt
gtgcacgtgtcaggattagtagacgaaattcgtgaaaacgatgaggttgaatttgacttacaagatggaagaaaaggat
taaacgcagtaaacgtaagagttatataataatactagagagagaacaaggaggggatgtttgcaaagagatttaagac
gagtttacttccgctgtttgccggcttccttttgctgtttcaccttgtattagccggtcctgcggcagcaagtgcggaa
acggctaacaaaagtaacgagctggatagcctttccttttcatataataatttcgaagaggacgattaa