LOCUS       BBa_K2069005            2221 bp    DNA     linear       10-May-2024
DEFINITION  RBS + Cpn60 + RBS + CspC + RBS + Antifungal GAFP-1 + double 
            terminator
ACCESSION   BBa_K2069005
VERSION     BBa_K2069005.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..21
                     /label=Start Codon
     misc_feature    1663..1668
                     /label=Double Stop Codon
     misc_feature    926..926
                     /label=A > G (Recode for SapI site)
     CDS             19..1668
                     /label=BBa_K538001
     misc_feature    1..1668
                     /label=BBa_K538101
     misc_feature    1681..1684
                     /label=conserved
     RBS             1677..1688
                     /label=BBa_B0034
     misc_feature    1695..1697
                     /label=Start Codon
     misc_feature    1884..1889
                     /label=Double Stop Codon
     misc_feature    1769..1769
                     /label=A > G (Recode for SapI site)
     misc_feature    1731..1748
                     /label=RNA binding motif (RPN1)
     misc_feature    1773..1787
                     /label=RNA binding motif (RPN2)
     CDS             1695..1889
                     /label=BBa_K538004
     misc_feature    1677..1889
                     /label=BBa_K538104
     misc_feature    1..1889
                     /label=BBa_K2069000
     RBS             1898..1913
                     /label=strong RBS K780002
     CDS             1914..1994
                     /label=Signal peptide
     CDS             1995..2036
                     /label=Propeptide
     CDS             2037..2084
                     /label=GAFP-1
     misc_feature    1898..2084
                     /label=BBa_K1364002
     stem_loop       2104..2147
                     /label=stem_loop
     misc_feature    2093..2172
                     /label=BBa_B0010
     terminator      2093..2172
                     /label=BBa_B0010
     stem_loop       2188..2207
                     /label=T7 TE
     misc_feature    2214..2214
                     /label=stop
     polyA_site      2208..2221
                     /label=polya
     misc_feature    2181..2221
                     /label=BBa_B0012
     terminator      2181..2221
                     /label=BBa_B0012
     terminator      2093..2221
                     /label=BBa_B0015
     misc_feature    1898..2221
                     /label=BBa_K1364007
ORIGIN
        1 aaagaggaga aatactagat ggctgctaaa gacgtattat ttggtgatag cgcacgcgca
       61 aaaatgttgg taggtgtaaa cattttagcc gacgcagtaa gagttacctt aggacctaaa
      121 ggtcgtaacg ttgttataga aaaatcattt ggtgcaccga tcatcaccaa agatggtgtt
      181 tctgttgcgc gtgaaatcga attgaaagac aaattcgaaa acatgggcgc acagatggtt
      241 aaggaagttg cttctcaagc caacgaccaa gccggtgacg gcacaacgac agcgactgta
      301 ctagcacagg cgattatcag cgaaggcttg aaatctgttg cggctggcat gaatccaatg
      361 gatcttaaac gtggtattga taaagctacg gctgctgttg ttgccgccat taaagaacaa
      421 gctcagcctt gcttggatac aaaagcaatc gctcaggtag ggacaatctc tgccaatgcc
      481 gatgaaacgg ttggtcgttt aattgctgaa gcgatggaaa aagtcggtaa agaaggtgtg
      541 attaccgttg aagaaggcaa aggccttgaa gacgagcttg atgttgtaga aggcatgcag
      601 ttcgatcgcg gttacttgtc tccgtacttc atcaacaacc aagaaaaaat gaccgtagaa
      661 atggaaaatc cattaattct attggttgat aagaaaattg ataaccttca agagctgttg
      721 ccaattcttg aaaacgtcgc taaatcaggt cgtccattat tgatcgttgc tgaagatgtt
      781 gaaggccaag cactagcaac attggtagta aacaacttgc gcggcacatt caaggttgca
      841 gcggttaaag cccctggttt tggcgatcgt cgtaaagcga tgttgcaaga tcttgccatc
      901 ttgacgggtg gtcaggttat ttctgaggag ctagggatgt ctttagaaac tgcggatcct
      961 tcttctttgg gtacggcaag caaggttgtt atcgataaag aaaacaccgt gattgttgat
     1021 ggcgcaggta ctgaagcaag cgttaatact cgtgttgacc agatccgtgc tgaaatcgaa
     1081 agctcgactt ctgattacga catcgaaaag ttacaagaac gcgttgctaa gcttgcgggc
     1141 ggcgttgccg tgattaaggt tggtgcgggt tctgaaatgg aaatgaaaga gaagaaagac
     1201 cgtgttgacg atgcacttca tgcaactcgc gcagcggttg aagaaggtgt tgttgcgggt
     1261 ggtggtgttg ctttgattcg cgcactctct tcagtaaccg ttgttggtga taacgaagat
     1321 caaaacgtcg gtattgcatt ggcacttcgt gcgatggaag ctcctatccg tcaaatcgcg
     1381 ggtaacgcag gtgctgaagg gtcagtggtt gttgataaag tgaaatctgg cacaggtagc
     1441 tttggtttta acgccagcac aggtgagtat ggcgatatga ttgcgatggg tattttagac
     1501 cctgcaaaag tcacgcgttc atctctacaa gccgcggcgt ctatcgcagg tttgatgatc
     1561 acaaccgaag ccatggttgc ggatgcgcct gttgaagaag gcgctggtgg tatgcctgat
     1621 atgggcggca tgggtggaat gggcggtatg cctggcatga tgtaataata ctagagaaag
     1681 aggagaaata ctagatgaat aaaggtaccg taaaattttt caatgaatct aaaggattcg
     1741 gatttatcac tgaagaagga aacaatgagg agcattttgt gcacgtgtca ggattagtag
     1801 acgaaattcg tgaaaacgat gaggttgaat ttgacttaca agatggaaga aaaggattaa
     1861 acgcagtaaa cgtaagagtt atataataat actagagaga gaacaaggag gggatgtttg
     1921 caaagagatt taagacgagt ttacttccgc tgtttgccgg cttccttttg ctgtttcacc
     1981 ttgtattagc cggtcctgcg gcagcaagtg cggaaacggc taacaaaagt aacgagctgg
     2041 atagcctttc cttttcatat aataatttcg aagaggacga ttaatactag agccaggcat
     2101 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     2161 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     2221 a
//