RBS + Cpn60 + RBS + CspC + RBS + Antifungal GAFP-1 + double terminator
assembly of parts BBa_K2069000 and BBa_K1364007, this last one from Tolouse 2014's project subtiTree project
This intermediate part allows the transcription of chaperonin 60, CspC and the GAFP-1 protein if added a promoter. This part has a double terminator (T1 E. coli and phage T7 early terminator)
RBS + Antifungal GAFP-1 + Double terminator
This was assembled with parts from the 2016 distribution kit
This intermediate part contains the assemble of Amsterdam's 2011 biobricks for their project icE. coli BBa_K538101 and BBa_K538104
There were no special issues for the design of this part
Double terminator consisting of BBa_B0010 and BBa_B0012
This gene was ordered from a synthesis company.
The Gastrodia anti-fungal protein (GAFP-1) is a mannose-binding lectin which is able to inhibit the growth of multiples species of plant pathogenic fungi. The RBS used is a strong RBS for Bacillus subtilis (K780002).
This part is composed of a Strong RBS (K780002) and the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program.
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Bas Stringer (Sequence by Paul van Dieken)
Conversion of the iGEM parts registry to SBOL2.1