BBa_K2069005
type: ComponentDefinition
persistent identity: BBa K2069005
display id: BBa_K2069005
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + Cpn60 + RBS + CspC + RBS + Antifungal GAFP-1 + double terminator
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T11:25:56Z
mutable provenance: assembly of parts BBa_K2069000 and BBa_K1364007, this last one from Tolouse 2014's project subtiTree project
owned by: james
owned by: myers
mutable description: This intermediate part allows the transcription of chaperonin 60, CspC and the GAFP-1 protein if added a promoter. This part has a double terminator (T1 E. coli and phage T7 early terminator)
discontinued: false
dominant: false
group u list: _2537_
m user id: 29482
owner id: 29482
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: no special issues in the design
star: false
creator: Mara Valverde Rasc??n
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K2069000
component: BBa_K1364007
sequence annotation: BBa_K2069000
sequence annotation: BBa_K1364007
sequence: https://synbiohub.org/public/igem/BBa_K2069005_sequence/1

BBa_K2069000
type: Component
persistent identity: component2494369
display id: component2494369
version: 1
top level: BBa_K2069005
definition: BBa_K2069000
access: public

BBa_K1364007
type: Component
persistent identity: component2494382
display id: component2494382
version: 1
top level: BBa_K2069005
definition: GAFP-1
access: public

BBa_K2069000
type: SequenceAnnotation
persistent identity: annotation2494369
display id: annotation2494369
version: 1
top level: BBa_K2069005
location: https://synbiohub.org/public/igem/BBa_K2069005/annotation2494369/range2494369/1
component: BBa_K2069000

https://synbiohub.org/public/igem/BBa_K2069005/annotation2494369/range2494369/1
type: Range
persistent identity: range2494369
display id: range2494369
version: 1
top level: BBa_K2069005
direction: direction/reverse
start: 1
end: 1889
orientation: inline

BBa_K1364007
type: SequenceAnnotation
persistent identity: annotation2494382
display id: annotation2494382
version: 1
top level: BBa_K2069005
location: https://synbiohub.org/public/igem/BBa_K2069005/annotation2494382/range2494382/1
component: BBa_K1364007

https://synbiohub.org/public/igem/BBa_K2069005/annotation2494382/range2494382/1
type: Range
persistent identity: range2494382
display id: range2494382
version: 1
top level: BBa_K2069005
direction: direction/reverse
start: 1898
end: 2221
orientation: inline

GAFP-1
type: ComponentDefinition
persistent identity: BBa K1364007
display id: BBa_K1364007
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + Antifungal GAFP-1 + Double terminator
created: 2014-10-02T11:00:00Z
modified: 2015-06-08T08:40:49Z
mutable provenance: bla
owned by: james
owned by: myers
mutable description: bla bla
discontinued: false
dominant: false
experience: experience/Works
group u list: _1740_
m user id: 4206
owner id: 21465
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: bla
star: false
creator: Jourdan Camille
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_B0015
component: BBa_K1364002
sequence annotation: BBa_K1364002
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K1364007_sequence/1

BBa_B0015
type: Component
persistent identity: component2392854
display id: component2392854
version: 1
top level: GAFP-1
definition: BBa_B0015
access: public

BBa_K1364002
type: Component
persistent identity: component2392847
display id: component2392847
version: 1
top level: GAFP-1
definition: GAFP-1
access: public

BBa_K1364002
type: SequenceAnnotation
persistent identity: annotation2392847
display id: annotation2392847
version: 1
top level: GAFP-1
location: https://synbiohub.org/public/igem/BBa_K1364007/annotation2392847/range2392847/1
component: BBa_K1364002

https://synbiohub.org/public/igem/BBa_K1364007/annotation2392847/range2392847/1
type: Range
persistent identity: range2392847
display id: range2392847
version: 1
top level: GAFP-1
direction: direction/reverse
start: 1
end: 187
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2392854
display id: annotation2392854
version: 1
top level: GAFP-1
location: https://synbiohub.org/public/igem/BBa_K1364007/annotation2392854/range2392854/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K1364007/annotation2392854/range2392854/1
type: Range
persistent identity: range2392854
display id: range2392854
version: 1
top level: GAFP-1
direction: direction/reverse
start: 196
end: 324
orientation: inline

BBa_K2069000
type: ComponentDefinition
persistent identity: BBa K2069000
display id: BBa_K2069000
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + Cpn60 + RBS + CspC
created: 2016-10-11T11:00:00Z
modified: 2016-10-12T10:20:29Z
mutable provenance: This was assembled with parts from the 2016 distribution kit
owned by: james
owned by: myers
mutable description: This intermediate part contains the assemble of Amsterdam's 2011 biobricks for their project icE. coli BBa_K538101 and BBa_K538104
discontinued: false
dominant: false
group u list: _2537_
m user id: 29482
owner id: 29482
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: There were no special issues for the design of this part
star: false
creator: Mara Valverde Rasc??n
type: DnaRegion
role: partType/Intermediate
role: SO:0000804
component: BBa_K538104
component: BBa_K538101
sequence annotation: BBa_K538101
sequence annotation: BBa_K538104
sequence: https://synbiohub.org/public/igem/BBa_K2069000_sequence/1

BBa_K538104
type: Component
persistent identity: component2494112
display id: component2494112
version: 1
top level: BBa_K2069000
definition: BBa_K538104
access: public

BBa_K538101
type: Component
persistent identity: component2494103
display id: component2494103
version: 1
top level: BBa_K2069000
definition: BBa_K538101
access: public

BBa_K538101
type: SequenceAnnotation
persistent identity: annotation2494103
display id: annotation2494103
version: 1
top level: BBa_K2069000
location: https://synbiohub.org/public/igem/BBa_K2069000/annotation2494103/range2494103/1
component: BBa_K538101

https://synbiohub.org/public/igem/BBa_K2069000/annotation2494103/range2494103/1
type: Range
persistent identity: range2494103
display id: range2494103
version: 1
top level: BBa_K2069000
direction: direction/reverse
start: 1
end: 1668
orientation: inline

BBa_K538104
type: SequenceAnnotation
persistent identity: annotation2494112
display id: annotation2494112
version: 1
top level: BBa_K2069000
location: https://synbiohub.org/public/igem/BBa_K2069000/annotation2494112/range2494112/1
component: BBa_K538104

https://synbiohub.org/public/igem/BBa_K2069000/annotation2494112/range2494112/1
type: Range
persistent identity: range2494112
display id: range2494112
version: 1
top level: BBa_K2069000
direction: direction/reverse
start: 1677
end: 1889
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

GAFP-1
type: ComponentDefinition
persistent identity: BBa K1364002
display id: BBa_K1364002
version: 1
was generated by: iGEM to SBOL conversion
description: RBS - Antifungal GAFP-1
created: 2014-09-30T11:00:00Z
modified: 2015-06-08T08:39:49Z
mutable provenance: This gene was ordered from a synthesis company.
owned by: james
owned by: myers
mutable description: The Gastrodia anti-fungal protein (GAFP-1) is a mannose-binding lectin which is able to inhibit the growth of multiples species of plant pathogenic fungi. The RBS used is a strong RBS for Bacillus subtilis (K780002).
discontinued: false
dominant: false
experience: experience/Works
group u list: _1740_
m user id: 4206
owner id: 21466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part is composed of a Strong RBS (K780002) and the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program.
star: false
creator: Manon Molina
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: strong RBS K780002
sequence annotation: Signal peptide
sequence annotation: Propeptide
sequence annotation: GAFP-1
sequence: https://synbiohub.org/public/igem/BBa_K1364002_sequence/1

strong RBS K780002
type: SequenceAnnotation
persistent identity: annotation2391440
display id: annotation2391440
version: 1
top level: GAFP-1
location: https://synbiohub.org/public/igem/BBa_K1364002/annotation2391440/range2391440/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K1364002/annotation2391440/range2391440/1
type: Range
persistent identity: range2391440
display id: range2391440
version: 1
top level: GAFP-1
direction: direction/forward
start: 1
end: 16
orientation: inline

Signal peptide
type: SequenceAnnotation
persistent identity: annotation2391441
display id: annotation2391441
version: 1
top level: GAFP-1
location: https://synbiohub.org/public/igem/BBa_K1364002/annotation2391441/range2391441/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1364002/annotation2391441/range2391441/1
type: Range
persistent identity: range2391441
display id: range2391441
version: 1
top level: GAFP-1
direction: direction/forward
start: 17
end: 97
orientation: inline

Propeptide
type: SequenceAnnotation
persistent identity: annotation2391442
display id: annotation2391442
version: 1
top level: GAFP-1
location: https://synbiohub.org/public/igem/BBa_K1364002/annotation2391442/range2391442/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K1364002/annotation2391442/range2391442/1
type: Range
persistent identity: range2391442
display id: range2391442
version: 1
top level: GAFP-1
direction: direction/forward
start: 98
end: 139
orientation: inline

GAFP-1
type: SequenceAnnotation
persistent identity: annotation2391443
display id: annotation2391443
version: 1
top level: GAFP-1
location: https://synbiohub.org/public/igem/BBa_K1364002/annotation2391443/range2391443/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K1364002/annotation2391443/range2391443/1
type: Range
persistent identity: range2391443
display id: range2391443
version: 1
top level: GAFP-1
direction: direction/forward
start: 140
end: 187
orientation: inline

BBa_K538101
type: ComponentDefinition
persistent identity: BBa K538101
display id: BBa_K538101
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + Cpn60
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: -
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Bas Stringer
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_K538001
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K538001
sequence: https://synbiohub.org/public/igem/BBa_K538101_sequence/1

BBa_K538001
type: Component
persistent identity: component2133885
display id: component2133885
version: 1
top level: BBa_K538101
definition: Cpn60
access: public

BBa_B0034
type: Component
persistent identity: component2133881
display id: component2133881
version: 1
top level: BBa_K538101
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2133881
display id: annotation2133881
version: 1
top level: BBa_K538101
location: https://synbiohub.org/public/igem/BBa_K538101/annotation2133881/range2133881/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538101/annotation2133881/range2133881/1
type: Range
persistent identity: range2133881
display id: range2133881
version: 1
top level: BBa_K538101
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K538001
type: SequenceAnnotation
persistent identity: annotation2133885
display id: annotation2133885
version: 1
top level: BBa_K538101
location: https://synbiohub.org/public/igem/BBa_K538101/annotation2133885/range2133885/1
component: BBa_K538001

https://synbiohub.org/public/igem/BBa_K538101/annotation2133885/range2133885/1
type: Range
persistent identity: range2133885
display id: range2133885
version: 1
top level: BBa_K538101
direction: direction/reverse
start: 19
end: 1668
orientation: inline

BBa_K538104
type: ComponentDefinition
persistent identity: BBa K538104
display id: BBa_K538104
version: 1
was generated by: iGEM to SBOL conversion
description: RBS + CspC
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: -
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -
discontinued: false
dominant: false
experience: experience/Works
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: -
star: false
creator: Bas Stringer
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_K538004
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_K538004
sequence: https://synbiohub.org/public/igem/BBa_K538104_sequence/1

BBa_K538004
type: Component
persistent identity: component2133910
display id: component2133910
version: 1
top level: BBa_K538104
definition: CspC
access: public

BBa_B0034
type: Component
persistent identity: component2133904
display id: component2133904
version: 1
top level: BBa_K538104
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2133904
display id: annotation2133904
version: 1
top level: BBa_K538104
location: https://synbiohub.org/public/igem/BBa_K538104/annotation2133904/range2133904/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K538104/annotation2133904/range2133904/1
type: Range
persistent identity: range2133904
display id: range2133904
version: 1
top level: BBa_K538104
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_K538004
type: SequenceAnnotation
persistent identity: annotation2133910
display id: annotation2133910
version: 1
top level: BBa_K538104
location: https://synbiohub.org/public/igem/BBa_K538104/annotation2133910/range2133910/1
component: BBa_K538004

https://synbiohub.org/public/igem/BBa_K538104/annotation2133910/range2133910/1
type: Range
persistent identity: range2133910
display id: range2133910
version: 1
top level: BBa_K538104
direction: direction/reverse
start: 19
end: 213
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

Cpn60
type: ComponentDefinition
persistent identity: BBa K538001
display id: BBa_K538001
version: 1
was generated by: iGEM to SBOL conversion
description: Cpn60 (O. antarctica)
created: 2011-07-28T11:00:00Z
modified: 2015-05-08T01:12:36Z
mutable provenance: De novo synthesis
owned by: james
owned by: myers
mutable description: -
discontinued: false
dominant: false
experience: experience/None
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: -
star: false
creator: Bas Stringer (Sequence by Paul van Dieken)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start Codon
sequence annotation: A > G (Recode for SapI site)
sequence annotation: Double Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K538001_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2124231
display id: annotation2124231
version: 1
top level: Cpn60
location: https://synbiohub.org/public/igem/BBa_K538001/annotation2124231/range2124231/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K538001/annotation2124231/range2124231/1
type: Range
persistent identity: range2124231
display id: range2124231
version: 1
top level: Cpn60
direction: direction/forward
start: 1
end: 3
orientation: inline

A > G (Recode for SapI site)
type: SequenceAnnotation
persistent identity: annotation2128866
display id: annotation2128866
version: 1
top level: Cpn60
location: https://synbiohub.org/public/igem/BBa_K538001/annotation2128866/range2128866/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K538001/annotation2128866/range2128866/1
type: Range
persistent identity: range2128866
display id: range2128866
version: 1
top level: Cpn60
direction: direction/forward
start: 908
end: 908
orientation: inline

Double Stop Codon
type: SequenceAnnotation
persistent identity: annotation2124232
display id: annotation2124232
version: 1
top level: Cpn60
location: https://synbiohub.org/public/igem/BBa_K538001/annotation2124232/range2124232/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K538001/annotation2124232/range2124232/1
type: Range
persistent identity: range2124232
display id: range2124232
version: 1
top level: Cpn60
direction: direction/forward
start: 1645
end: 1650
orientation: inline

CspC
type: ComponentDefinition
persistent identity: BBa K538004
display id: BBa_K538004
version: 1
was generated by: iGEM to SBOL conversion
description: CspC (P. irgensii)
created: 2011-09-07T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: -
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: -
discontinued: false
dominant: false
experience: experience/Works
group u list: _704_
m user id: 0
owner id: 9985
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: -
star: false
creator: Bas Stringer (Sequence by Paul van Dieken)
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: RNA binding motif (RPN1)
sequence annotation: A > G (Recode for SapI site)
sequence annotation: RNA binding motif (RPN2)
sequence annotation: Double Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_K538004_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2127783
display id: annotation2127783
version: 1
top level: CspC
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2127783/range2127783/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_K538004/annotation2127783/range2127783/1
type: Range
persistent identity: range2127783
display id: range2127783
version: 1
top level: CspC
direction: direction/forward
start: 1
end: 3
orientation: inline

RNA binding motif (RPN1)
type: SequenceAnnotation
persistent identity: annotation2129605
display id: annotation2129605
version: 1
top level: CspC
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2129605/range2129605/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K538004/annotation2129605/range2129605/1
type: Range
persistent identity: range2129605
display id: range2129605
version: 1
top level: CspC
direction: direction/forward
start: 37
end: 54
orientation: inline

A > G (Recode for SapI site)
type: SequenceAnnotation
persistent identity: annotation2127785
display id: annotation2127785
version: 1
top level: CspC
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2127785/range2127785/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K538004/annotation2127785/range2127785/1
type: Range
persistent identity: range2127785
display id: range2127785
version: 1
top level: CspC
direction: direction/forward
start: 75
end: 75
orientation: inline

RNA binding motif (RPN2)
type: SequenceAnnotation
persistent identity: annotation2129606
display id: annotation2129606
version: 1
top level: CspC
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2129606/range2129606/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K538004/annotation2129606/range2129606/1
type: Range
persistent identity: range2129606
display id: range2129606
version: 1
top level: CspC
direction: direction/forward
start: 79
end: 93
orientation: inline

Double Stop Codon
type: SequenceAnnotation
persistent identity: annotation2127784
display id: annotation2127784
version: 1
top level: CspC
location: https://synbiohub.org/public/igem/BBa_K538004/annotation2127784/range2127784/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K538004/annotation2127784/range2127784/1
type: Range
persistent identity: range2127784
display id: range2127784
version: 1
top level: CspC
direction: direction/forward
start: 190
end: 195
orientation: inline

https://synbiohub.org/public/igem/BBa_K2069005_sequence/1
type: Sequence
persistent identity: BBa K2069005 sequence
display id: BBa_K2069005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataatactagagaaagaggagaaatactagatgaataaaggtaccgtaaaatttttcaatgaatctaaaggattcggatttatcactgaagaaggaaacaatgaggagcattttgtgcacgtgtcaggattagtagacgaaattcgtgaaaacgatgaggttgaatttgacttacaagatggaagaaaaggattaaacgcagtaaacgtaagagttatataataatactagagagagaacaaggaggggatgtttgcaaagagatttaagacgagtttacttccgctgtttgccggcttccttttgctgtttcaccttgtattagccggtcctgcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1364007_sequence/1
type: Sequence
persistent identity: BBa K1364007 sequence
display id: BBa_K1364007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaacaaggaggggatgtttgcaaagagatttaagacgagtttacttccgctgtttgccggcttccttttgctgtttcaccttgtattagccggtcctgcggcagcaagtgcggaaacggctaacaaaagtaacgagctggatagcctttccttttcatataataatttcgaagaggacgattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2069000_sequence/1
type: Sequence
persistent identity: BBa K2069000 sequence
display id: BBa_K2069000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataatactagagaaagaggagaaatactagatgaataaaggtaccgtaaaatttttcaatgaatctaaaggattcggatttatcactgaagaaggaaacaatgaggagcattttgtgcacgtgtcaggattagtagacgaaattcgtgaaaacgatgaggttgaatttgacttacaagatggaagaaaaggattaaacgcagtaaacgtaagagttatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1364002_sequence/1
type: Sequence
persistent identity: BBa K1364002 sequence
display id: BBa_K1364002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agagaacaaggaggggatgtttgcaaagagatttaagacgagtttacttccgctgtttgccggcttccttttgctgtttcaccttgtattagccggtcctgcggcagcaagtgcggaaacggctaacaaaagtaacgagctggatagcctttccttttcatataataatttcgaagaggacgattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K538101_sequence/1
type: Sequence
persistent identity: BBa K538101 sequence
display id: BBa_K538101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K538104_sequence/1
type: Sequence
persistent identity: BBa K538104 sequence
display id: BBa_K538104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaataaaggtaccgtaaaatttttcaatgaatctaaaggattcggatttatcactgaagaaggaaacaatgaggagcattttgtgcacgtgtcaggattagtagacgaaattcgtgaaaacgatgaggttgaatttgacttacaagatggaagaaaaggattaaacgcagtaaacgtaagagttatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K538001_sequence/1
type: Sequence
persistent identity: BBa K538001 sequence
display id: BBa_K538001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K538004_sequence/1
type: Sequence
persistent identity: BBa K538004 sequence
display id: BBa_K538004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaataaaggtaccgtaaaatttttcaatgaatctaaaggattcggatttatcactgaagaaggaaacaatgaggagcattttgtgcacgtgtcaggattagtagacgaaattcgtgaaaacgatgaggttgaatttgacttacaagatggaagaaaaggattaaacgcagtaaacgtaagagttatataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z