LOCUS BBa_K2069006 3445 bp DNA linear 15-Nov-2024 DEFINITION pT7 + RBS + Reflectin (+LipA tag) + RBS + Cpn10 + RBS + Cpn60 + RBS + CspC + RBS + Antifungal GAFP-1 ACCESSION BBa_K2069006 VERSION BBa_K2069006.1 FEATURES Location/Qualifiers misc_feature 21..32 /label=B0034 promoter 1..20 /label=T7 promoter RBS 24..27 /label=RBS promoter 1..32 /label=BBa_K525998 CDS 39..893 /label=Reflectin misc_feature 39..893 /label=BBa_K541506 misc_feature 1..893 /label=BBa_K2069001 misc_feature 906..909 /label=conserved RBS 902..913 /label=BBa_B0034 misc_feature 920..922 /label=Start Codon misc_feature 1211..1216 /label=Double Stop Codon CDS 920..1216 /label=BBa_K538000 misc_feature 902..1216 /label=BBa_K538100 misc_feature 1..1216 /label=BBa_K2069003 misc_feature 1229..1232 /label=conserved RBS 1225..1236 /label=BBa_B0034 misc_feature 1243..1245 /label=Start Codon misc_feature 2887..2892 /label=Double Stop Codon misc_feature 2150..2150 /label=A > G (Recode for SapI site) CDS 1243..2892 /label=BBa_K538001 misc_feature 1225..2892 /label=BBa_K538101 misc_feature 2905..2908 /label=conserved RBS 2901..2912 /label=BBa_B0034 misc_feature 2919..2921 /label=Start Codon misc_feature 3108..3113 /label=Double Stop Codon misc_feature 2993..2993 /label=A > G (Recode for SapI site) misc_feature 2955..2972 /label=RNA binding motif (RPN1) misc_feature 2997..3011 /label=RNA binding motif (RPN2) CDS 2919..3113 /label=BBa_K538004 misc_feature 2901..3113 /label=BBa_K538104 misc_feature 1225..3113 /label=BBa_K2069000 RBS 3122..3137 /label=strong RBS K780002 CDS 3138..3218 /label=Signal peptide CDS 3219..3260 /label=Propeptide CDS 3261..3308 /label=GAFP-1 misc_feature 3122..3308 /label=BBa_K1364002 stem_loop 3328..3371 /label=stem_loop misc_feature 3317..3396 /label=BBa_B0010 terminator 3317..3396 /label=BBa_B0010 stem_loop 3412..3431 /label=T7 TE misc_feature 3438..3438 /label=stop polyA_site 3432..3445 /label=polya misc_feature 3405..3445 /label=BBa_B0012 terminator 3405..3445 /label=BBa_B0012 terminator 3317..3445 /label=BBa_B0015 misc_feature 3122..3445 /label=BBa_K1364007 misc_feature 1225..3445 /label=BBa_K2069005 ORIGIN 1 taatacgact cactataggg aaagaggaga aatactagat gaatcgcttt atgaatcgct 61 atcgcccgat gttcaataat atgtactcta acatgtaccg tggccgctac cgcggtatga 121 tggaaccgat gagccgcatg actatggact ttcagggccg ctacatggac agccaaggcc 181 gcatggtgga cccgcgctac tacgattact acggccgctt caacgattac gaccgctact 241 atggccgcag catgttcaac tatggctgga tgatggacgg cgatcgctac aaccgctata 301 accgctggat ggattacccg gagcgctaca tggatatgtc tggctaccag atggatatgt 361 ctggccgctg gatggatatg cagggccgcc actgcaaccc gtattctcag tggatgatgt 421 acaactacaa ccgccatggc tactacccga actactctta cggccgccac atgttttatc 481 cggaacgctg gatggacatg tctaactact ctatggacat gtatggccgc tatatggatc 541 gctggggccg ctactgtaac ccgttttctc agtacatgaa ctactacggc cgctactgga 601 actacccggg ctacaataat tactactatt ctcgcaacat gtattacccg gaacgctact 661 ttgacatgtc taactggcag atggacatgc aaggccgctg gatggacaac cagggccgct 721 attgttcccc gtactggaac aactggtacg gccgccatat gtactacccg taccagaaca 781 actacttcta cggccgctat gactacccgg gcatggacta ttccaactac caaatggaca 841 tgcagggccg ctacatggac cagtacggca tgaacgacta ctactattaa taatactaga 901 gaaagaggag aaatactaga tgaaaatccg tccattacat gatcgtattg ttgttcgccg 961 taaagaagaa gagaccgcaa ctgcgggtgg tattatttta ccgggcgctg cggcagaaaa 1021 accaaatcaa ggtgttgtta tctctgtggg tactggccgt attcttgata atggttcagt 1081 gcaagcgctg gcggttaacg aaggcgatgt tgtcgttttt ggtaaatact caggtcaaaa 1141 tactatcgat atcgatggtg aagaattatt gattttgaat gaaagtgata tctacggcgt 1201 tttagaagct taataatact agagaaagag gagaaatact agatggctgc taaagacgta 1261 ttatttggtg atagcgcacg cgcaaaaatg ttggtaggtg taaacatttt agccgacgca 1321 gtaagagtta ccttaggacc taaaggtcgt aacgttgtta tagaaaaatc atttggtgca 1381 ccgatcatca ccaaagatgg tgtttctgtt gcgcgtgaaa tcgaattgaa agacaaattc 1441 gaaaacatgg gcgcacagat ggttaaggaa gttgcttctc aagccaacga ccaagccggt 1501 gacggcacaa cgacagcgac tgtactagca caggcgatta tcagcgaagg cttgaaatct 1561 gttgcggctg gcatgaatcc aatggatctt aaacgtggta ttgataaagc tacggctgct 1621 gttgttgccg ccattaaaga acaagctcag ccttgcttgg atacaaaagc aatcgctcag 1681 gtagggacaa tctctgccaa tgccgatgaa acggttggtc gtttaattgc tgaagcgatg 1741 gaaaaagtcg gtaaagaagg tgtgattacc gttgaagaag gcaaaggcct tgaagacgag 1801 cttgatgttg tagaaggcat gcagttcgat cgcggttact tgtctccgta cttcatcaac 1861 aaccaagaaa aaatgaccgt agaaatggaa aatccattaa ttctattggt tgataagaaa 1921 attgataacc ttcaagagct gttgccaatt cttgaaaacg tcgctaaatc aggtcgtcca 1981 ttattgatcg ttgctgaaga tgttgaaggc caagcactag caacattggt agtaaacaac 2041 ttgcgcggca cattcaaggt tgcagcggtt aaagcccctg gttttggcga tcgtcgtaaa 2101 gcgatgttgc aagatcttgc catcttgacg ggtggtcagg ttatttctga ggagctaggg 2161 atgtctttag aaactgcgga tccttcttct ttgggtacgg caagcaaggt tgttatcgat 2221 aaagaaaaca ccgtgattgt tgatggcgca ggtactgaag caagcgttaa tactcgtgtt 2281 gaccagatcc gtgctgaaat cgaaagctcg acttctgatt acgacatcga aaagttacaa 2341 gaacgcgttg ctaagcttgc gggcggcgtt gccgtgatta aggttggtgc gggttctgaa 2401 atggaaatga aagagaagaa agaccgtgtt gacgatgcac ttcatgcaac tcgcgcagcg 2461 gttgaagaag gtgttgttgc gggtggtggt gttgctttga ttcgcgcact ctcttcagta 2521 accgttgttg gtgataacga agatcaaaac gtcggtattg cattggcact tcgtgcgatg 2581 gaagctccta tccgtcaaat cgcgggtaac gcaggtgctg aagggtcagt ggttgttgat 2641 aaagtgaaat ctggcacagg tagctttggt tttaacgcca gcacaggtga gtatggcgat 2701 atgattgcga tgggtatttt agaccctgca aaagtcacgc gttcatctct acaagccgcg 2761 gcgtctatcg caggtttgat gatcacaacc gaagccatgg ttgcggatgc gcctgttgaa 2821 gaaggcgctg gtggtatgcc tgatatgggc ggcatgggtg gaatgggcgg tatgcctggc 2881 atgatgtaat aatactagag aaagaggaga aatactagat gaataaaggt accgtaaaat 2941 ttttcaatga atctaaagga ttcggattta tcactgaaga aggaaacaat gaggagcatt 3001 ttgtgcacgt gtcaggatta gtagacgaaa ttcgtgaaaa cgatgaggtt gaatttgact 3061 tacaagatgg aagaaaagga ttaaacgcag taaacgtaag agttatataa taatactaga 3121 gagagaacaa ggaggggatg tttgcaaaga gatttaagac gagtttactt ccgctgtttg 3181 ccggcttcct tttgctgttt caccttgtat tagccggtcc tgcggcagca agtgcggaaa 3241 cggctaacaa aagtaacgag ctggatagcc tttccttttc atataataat ttcgaagagg 3301 acgattaata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 3361 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 3421 tcgggtgggc ctttctgcgt ttata //