pT7 + RBS + Reflectin (+LipA tag) + RBS + Cpn10 + RBS + Cpn60 + RBS + CspC + RBS + Antifungal GAFP-1
This is team Tec-Chihuahua gene expression generator. It expresses the chaperonins of the Cpn10/Cpn60 system for expression of proteins in atypical temperatures as well as the CspC protein, all with a strong RBS and pT7 promoter for high expression. It also expresses the GAFP-1 from Gastrodia and contains a t1 terminator as well as the early T7 phage terminator.
pT7 + RBS + Reflectin (+LipA tag) + RBS + Cpn10
assembly of parts from the 2016 distribution kit
This part allows the expression of Reflectin and Cpn10 with the addition of a terminator. It caontains the part BBa_K538100 from Amsterdam's 2011 project icE. coli
RBS + Cpn60 + RBS + CspC + RBS + Antifungal GAFP-1 + double terminator
assembly of parts BBa_K2069000 and BBa_K1364007, this last one from Tolouse 2014's project subtiTree project
This intermediate part allows the transcription of chaperonin 60, CspC and the GAFP-1 protein if added a promoter. This part has a double terminator (T1 E. coli and phage T7 early terminator)
RBS + Antifungal GAFP-1 + Double terminator
This was assembled with parts from the 2016 distribution kit
This intermediate part contains the assemble of Amsterdam's 2011 biobricks for their project icE. coli BBa_K538101 and BBa_K538104
There were no special issues for the design of this part
The subparts were obtained from the 2016 distribution kit
This part can express reflectin in the outer layer of bacteria, and was used in our project to assemble our final biobrick
RBS (Elowitz 1999) -- defines RBS efficiency
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Double terminator consisting of BBa_B0010 and BBa_B0012
This gene was ordered from a synthesis company.
The Gastrodia anti-fungal protein (GAFP-1) is a mannose-binding lectin which is able to inhibit the growth of multiples species of plant pathogenic fungi. The RBS used is a strong RBS for Bacillus subtilis (K780002).
This part is composed of a Strong RBS (K780002) and the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program.
Reflectin is a self-assembling protein that has the ability to reflect the sun light which depends on the thickness of the protein layer. Therefore, we thought that this ability could be used as a novel reporter for B.subtilis and E.coli.
Optimized for both B.subtilis and E.coli
This part encodes the protein ''cochaperonin 10'', which is part of the Cpn10/Cpn60 chaperonin system of ''Oleispira antarctica''. [More information will follow soon.]
The protein's sequence[1] was published by Ferrer ''et al.'' in 2004. Pre- and suffixes were added to this as clarified in OpenWetWare's BioBrick standards[2] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10[3] was confirmed using the EMBOSS recoder[4], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI. 1 - http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts 2 - http://openwetware.org/wiki/Biobrick_standard 3 - http://partsregistry.org/Help:Assembly_standard_10 4 - http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html
Bas Stringer (Sequence by Paul van Dieken)
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
Conversion of the iGEM parts registry to SBOL2.1