LOCUS       BBa_K2069007            1225 bp    DNA     linear       10-May-2024
DEFINITION  Expression of reflectin and GAFP-1
ACCESSION   BBa_K2069007
VERSION     BBa_K2069007.1  
FEATURES             Location/Qualifiers
     misc_feature    21..32
                     /label=B0034
     promoter        1..20
                     /label=T7 promoter
     RBS             24..27
                     /label=RBS
     promoter        1..32
                     /label=BBa_K525998
     CDS             39..893
                     /label=Reflectin
     misc_feature    39..893
                     /label=BBa_K541506
     misc_feature    1..893
                     /label=BBa_K2069001
     RBS             902..917
                     /label=strong RBS K780002
     CDS             918..998
                     /label=Signal peptide
     CDS             999..1040
                     /label=Propeptide
     CDS             1041..1088
                     /label=GAFP-1
     misc_feature    902..1088
                     /label=BBa_K1364002
     stem_loop       1108..1151
                     /label=stem_loop
     misc_feature    1097..1176
                     /label=BBa_B0010
     terminator      1097..1176
                     /label=BBa_B0010
     stem_loop       1192..1211
                     /label=T7 TE
     misc_feature    1218..1218
                     /label=stop
     polyA_site      1212..1225
                     /label=polya
     misc_feature    1185..1225
                     /label=BBa_B0012
     terminator      1185..1225
                     /label=BBa_B0012
     terminator      1097..1225
                     /label=BBa_B0015
     misc_feature    902..1225
                     /label=BBa_K1364007
ORIGIN
        1 taatacgact cactataggg aaagaggaga aatactagat gaatcgcttt atgaatcgct
       61 atcgcccgat gttcaataat atgtactcta acatgtaccg tggccgctac cgcggtatga
      121 tggaaccgat gagccgcatg actatggact ttcagggccg ctacatggac agccaaggcc
      181 gcatggtgga cccgcgctac tacgattact acggccgctt caacgattac gaccgctact
      241 atggccgcag catgttcaac tatggctgga tgatggacgg cgatcgctac aaccgctata
      301 accgctggat ggattacccg gagcgctaca tggatatgtc tggctaccag atggatatgt
      361 ctggccgctg gatggatatg cagggccgcc actgcaaccc gtattctcag tggatgatgt
      421 acaactacaa ccgccatggc tactacccga actactctta cggccgccac atgttttatc
      481 cggaacgctg gatggacatg tctaactact ctatggacat gtatggccgc tatatggatc
      541 gctggggccg ctactgtaac ccgttttctc agtacatgaa ctactacggc cgctactgga
      601 actacccggg ctacaataat tactactatt ctcgcaacat gtattacccg gaacgctact
      661 ttgacatgtc taactggcag atggacatgc aaggccgctg gatggacaac cagggccgct
      721 attgttcccc gtactggaac aactggtacg gccgccatat gtactacccg taccagaaca
      781 actacttcta cggccgctat gactacccgg gcatggacta ttccaactac caaatggaca
      841 tgcagggccg ctacatggac cagtacggca tgaacgacta ctactattaa taatactaga
      901 gagagaacaa ggaggggatg tttgcaaaga gatttaagac gagtttactt ccgctgtttg
      961 ccggcttcct tttgctgttt caccttgtat tagccggtcc tgcggcagca agtgcggaaa
     1021 cggctaacaa aagtaacgag ctggatagcc tttccttttc atataataat ttcgaagagg
     1081 acgattaata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1141 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1201 tcgggtgggc ctttctgcgt ttata
//