BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916612 1 BBa_B0012 component1916610 1 BBa_B0010 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_K1364007 1 GAFP-1 RBS + Antifungal GAFP-1 + Double terminator 2014-10-02T11:00:00Z 2015-06-08T08:40:49Z bla bla bla false false _1740_ 4206 21465 9 In stock false bla false Jourdan Camille component2392854 1 BBa_B0015 component2392847 1 BBa_K1364002 annotation2392854 1 BBa_B0015 range2392854 1 196 324 annotation2392847 1 BBa_K1364002 range2392847 1 1 187 BBa_K1364002 1 GAFP-1 RBS - Antifungal GAFP-1 2014-09-30T11:00:00Z 2015-06-08T08:39:49Z This gene was ordered from a synthesis company. The Gastrodia anti-fungal protein (GAFP-1) is a mannose-binding lectin which is able to inhibit the growth of multiples species of plant pathogenic fungi. The RBS used is a strong RBS for Bacillus subtilis (K780002). false false _1740_ 4206 21466 9 In stock false This part is composed of a Strong RBS (K780002) and the open reading frame of the Gastrodia anti-fungal protein 1 (GAFP-1) optimized for its expression and its secretion in Bacillus subtilis. The codon optimization was made thanks to the DNA 2.0 software program. false Manon Molina annotation2391441 1 Signal peptide range2391441 1 17 97 annotation2391443 1 GAFP-1 range2391443 1 140 187 annotation2391442 1 Propeptide range2391442 1 98 139 annotation2391440 1 strong RBS K780002 range2391440 1 1 16 BBa_K525998 1 pT7 Promoter T7 and RBS 2011-09-12T11:00:00Z 2015-05-08T01:12:35Z Synthesis Released HQ 2013 Promoter T7 and RBS false false _690_ 0 8543 9 In stock false Synthesis false Anna Drong annotation2129432 1 T7 promoter range2129432 1 1 20 annotation2127788 1 B0034 range2127788 1 21 32 annotation2129433 1 RBS range2129433 1 24 27 BBa_K2069007 1 BBa_K2069007 Expression of reflectin and GAFP-1 2016-10-13T11:00:00Z 2016-10-13T11:23:42Z parts from the registry This part allow the expression of reflectin and GAFP-1 in organisms capable of expressing proteins with a T7 promoter false false _2537_ 29482 29482 9 false No special issues in the design false Mara Valverde Rasc??n component2500712 1 BBa_K2069001 component2500725 1 BBa_K1364007 annotation2500725 1 BBa_K1364007 range2500725 1 902 1225 annotation2500712 1 BBa_K2069001 range2500712 1 1 893 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_K2069001 1 BBa_K2069001 pT7 + RBS + Reflectin (+LipA tag) 2016-10-11T11:00:00Z 2016-10-12T10:33:53Z The subparts were obtained from the 2016 distribution kit This part can express reflectin in the outer layer of bacteria, and was used in our project to assemble our final biobrick false false _2537_ 29482 29482 9 false No special issues regarding the design false Mara Valverde Rasc??n component2494129 1 BBa_K541506 component2494127 1 BBa_K525998 annotation2494129 1 BBa_K541506 range2494129 1 39 893 annotation2494127 1 BBa_K525998 range2494127 1 1 32 BBa_K541506 1 BBa_K541506 Reflectin1A from Cephalopod 2011-07-18T11:00:00Z 2015-05-08T01:12:38Z Cephalopod Released HQ 2013 Reflectin is a self-assembling protein that has the ability to reflect the sun light which depends on the thickness of the protein layer. Therefore, we thought that this ability could be used as a novel reporter for B.subtilis and E.coli. false false _708_ 0 8463 9 In stock false Optimized for both B.subtilis and E.coli false Fazilet Guler annotation2123461 1 Reflectin range2123461 1 1 855 BBa_K541506_sequence 1 atgaatcgctttatgaatcgctatcgcccgatgttcaataatatgtactctaacatgtaccgtggccgctaccgcggtatgatggaaccgatgagccgcatgactatggactttcagggccgctacatggacagccaaggccgcatggtggacccgcgctactacgattactacggccgcttcaacgattacgaccgctactatggccgcagcatgttcaactatggctggatgatggacggcgatcgctacaaccgctataaccgctggatggattacccggagcgctacatggatatgtctggctaccagatggatatgtctggccgctggatggatatgcagggccgccactgcaacccgtattctcagtggatgatgtacaactacaaccgccatggctactacccgaactactcttacggccgccacatgttttatccggaacgctggatggacatgtctaactactctatggacatgtatggccgctatatggatcgctggggccgctactgtaacccgttttctcagtacatgaactactacggccgctactggaactacccgggctacaataattactactattctcgcaacatgtattacccggaacgctactttgacatgtctaactggcagatggacatgcaaggccgctggatggacaaccagggccgctattgttccccgtactggaacaactggtacggccgccatatgtactacccgtaccagaacaactacttctacggccgctatgactacccgggcatggactattccaactaccaaatggacatgcagggccgctacatggaccagtacggcatgaacgactactactattaataa BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1364007_sequence 1 agagaacaaggaggggatgtttgcaaagagatttaagacgagtttacttccgctgtttgccggcttccttttgctgtttcaccttgtattagccggtcctgcggcagcaagtgcggaaacggctaacaaaagtaacgagctggatagcctttccttttcatataataatttcgaagaggacgattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K525998_sequence 1 taatacgactcactatagggaaagaggagaaa BBa_K1364002_sequence 1 agagaacaaggaggggatgtttgcaaagagatttaagacgagtttacttccgctgtttgccggcttccttttgctgtttcaccttgtattagccggtcctgcggcagcaagtgcggaaacggctaacaaaagtaacgagctggatagcctttccttttcatataataatttcgaagaggacgattaa BBa_K2069001_sequence 1 taatacgactcactatagggaaagaggagaaatactagatgaatcgctttatgaatcgctatcgcccgatgttcaataatatgtactctaacatgtaccgtggccgctaccgcggtatgatggaaccgatgagccgcatgactatggactttcagggccgctacatggacagccaaggccgcatggtggacccgcgctactacgattactacggccgcttcaacgattacgaccgctactatggccgcagcatgttcaactatggctggatgatggacggcgatcgctacaaccgctataaccgctggatggattacccggagcgctacatggatatgtctggctaccagatggatatgtctggccgctggatggatatgcagggccgccactgcaacccgtattctcagtggatgatgtacaactacaaccgccatggctactacccgaactactcttacggccgccacatgttttatccggaacgctggatggacatgtctaactactctatggacatgtatggccgctatatggatcgctggggccgctactgtaacccgttttctcagtacatgaactactacggccgctactggaactacccgggctacaataattactactattctcgcaacatgtattacccggaacgctactttgacatgtctaactggcagatggacatgcaaggccgctggatggacaaccagggccgctattgttccccgtactggaacaactggtacggccgccatatgtactacccgtaccagaacaactacttctacggccgctatgactacccgggcatggactattccaactaccaaatggacatgcagggccgctacatggaccagtacggcatgaacgactactactattaataa BBa_K2069007_sequence 1 taatacgactcactatagggaaagaggagaaatactagatgaatcgctttatgaatcgctatcgcccgatgttcaataatatgtactctaacatgtaccgtggccgctaccgcggtatgatggaaccgatgagccgcatgactatggactttcagggccgctacatggacagccaaggccgcatggtggacccgcgctactacgattactacggccgcttcaacgattacgaccgctactatggccgcagcatgttcaactatggctggatgatggacggcgatcgctacaaccgctataaccgctggatggattacccggagcgctacatggatatgtctggctaccagatggatatgtctggccgctggatggatatgcagggccgccactgcaacccgtattctcagtggatgatgtacaactacaaccgccatggctactacccgaactactcttacggccgccacatgttttatccggaacgctggatggacatgtctaactactctatggacatgtatggccgctatatggatcgctggggccgctactgtaacccgttttctcagtacatgaactactacggccgctactggaactacccgggctacaataattactactattctcgcaacatgtattacccggaacgctactttgacatgtctaactggcagatggacatgcaaggccgctggatggacaaccagggccgctattgttccccgtactggaacaactggtacggccgccatatgtactacccgtaccagaacaactacttctacggccgctatgactacccgggcatggactattccaactaccaaatggacatgcagggccgctacatggaccagtacggcatgaacgactactactattaataatactagagagagaacaaggaggggatgtttgcaaagagatttaagacgagtttacttccgctgtttgccggcttccttttgctgtttcaccttgtattagccggtcctgcggcagcaagtgcggaaacggctaacaaaagtaacgagctggatagcctttccttttcatataataatttcgaagaggacgattaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z