LOCUS       BBa_K2070005            1756 bp    DNA     linear       22-Nov-2024
DEFINITION  Constitutive ecf35 generator with ecf35 inducible GFP reporter
ACCESSION   BBa_K2070005
VERSION     BBa_K2070005.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..647
                     /label=BBa_K2070009
     stem_loop       667..710
                     /label=stem_loop
     misc_feature    656..735
                     /label=BBa_B0010
     terminator      656..735
                     /label=BBa_B0010
     stem_loop       751..770
                     /label=T7 TE
     misc_feature    777..777
                     /label=stop
     polyA_site      771..784
                     /label=polya
     misc_feature    744..784
                     /label=BBa_B0012
     terminator      744..784
                     /label=BBa_B0012
     terminator      656..784
                     /label=BBa_B0015
     misc_feature    1..784
                     /label=BBa_K2070013
     promoter        793..872
                     /label=BBa_K2070008
     RBS             881..893
                     /label=RBS-3\Weak
     RBS             887..890
                     /label=RBS
     misc_feature    881..893
                     /label=BBa_B0032
     RBS             881..893
                     /label=BBa_B0032
     CDS             900..1619
                     /label=GFP protein
     CDS             900..1619
                     /label=BBa_E0040
     stem_loop       1639..1682
                     /label=stem_loop
     misc_feature    1628..1707
                     /label=BBa_B0010
     terminator      1628..1707
                     /label=BBa_B0010
     stem_loop       1723..1742
                     /label=T7 TE
     misc_feature    1749..1749
                     /label=stop
     polyA_site      1743..1756
                     /label=polya
     misc_feature    1716..1756
                     /label=BBa_B0012
     terminator      1716..1756
                     /label=BBa_B0012
     misc_feature    881..1756
                     /label=BBa_E0240
     misc_feature    793..1756
                     /label=BBa_K2070004
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatgcatgc gagcaccagc gcgaccaccg cggcgggcag cgatgaaacc ctgctggcgc
      121 gctatcgcaa aggcgatagc gcggcgtttg aagtgctgta tcagcgccat cgccagggcc
      181 tgtatcgctt tctgtgcggc ctggcgggcc agaccgaact ggcggatgaa atttatcagg
      241 aaacctggct gagcctgatt cgcagcgaaa gccagccgca gggccgcgcg agctttcgca
      301 cctggctgta tcagattgcg cgcaaccgcc tgattgatca ttggcgcaaa catggccagc
      361 gccagccgct tcaggatagc tatgatgaac agctgcatgc gcagccggat cagggcgcgg
      421 gcccggaaca gcagctgagc ctgagccgcg atggcgaacg ccttcagagc gcgctggaaa
      481 ccctgccgga agaacagcgc gaagtgtttc tgatgcgcgc gcatggcgaa ctggaactgg
      541 cggaaattgc gaccctgacc cagagcccgc tggaaaccgt gaaaagccgc tttcgctatg
      601 cggtgcagaa actgcgccgc ctgctggcgg aagaagtggc ggtgtaatac tagagccagg
      661 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      721 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      781 tatatactag agaccatccg tattttttta gggatttata aaacttttcc atggcgtgcg
      841 tcgtctaata aatgggaagg aggaaatgat gttactagag tcacacagga aagtactaga
      901 tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
      961 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca acatacggaa
     1021 aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
     1081 tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat atgaaacagc
     1141 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact atatttttca
     1201 aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta
     1261 atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt ggacacaaat
     1321 tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa
     1381 tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa ctagcagacc
     1441 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
     1501 tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac atggtccttc
     1561 ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac aaataataat
     1621 actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     1681 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     1741 cctttctgcg tttata
//