BBa_K2070004 1 BBa_K2070004 ecf35 inducible GFP reporter 2016-10-08T11:00:00Z 2016-10-20T05:11:57Z Assembly This part contains ecf16 promoter,RBS,GFP and double terminator. false false _2538_ 31975 31975 9 false For detail, see our wiki. false iGEM UT-Tokyo 2016 component2525170 1 BBa_E0240 component2525159 1 BBa_K2070008 annotation2525170 1 BBa_E0240 range2525170 1 89 964 annotation2525159 1 BBa_K2070008 range2525159 1 1 80 BBa_K2070008 1 BBa_K2070008 ecf sigma30 promoter 2016-10-13T11:00:00Z 2016-10-14T06:14:37Z To be edited To be edited false false _2538_ 31975 31975 9 false To be edited false iGEM UT-Tokyo 2016 BBa_K2070013 1 BBa_K2070013 Constitutive ecf35 generator 2016-10-13T11:00:00Z 2016-10-20T05:27:43Z to be edited to be edited false false _2538_ 31975 31975 9 false to be edited false iGEM UT-Tokyo 2016 component2525211 1 BBa_B0032 component2525209 1 BBa_J23101 component2525220 1 BBa_B0015 component2525213 1 BBa_K2070009 annotation2525211 1 BBa_B0032 range2525211 1 44 56 annotation2525209 1 BBa_J23101 range2525209 1 1 35 annotation2525220 1 BBa_B0015 range2525220 1 656 784 annotation2525213 1 BBa_K2070009 range2525213 1 63 647 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K2070005 1 BBa_K2070005 Constitutive ecf35 generator with ecf35 inducible GFP reporter 2016-10-19T11:00:00Z 2016-10-20T05:57:45Z Asssembly This part was used to test the functionality of ECF sigma35 and its cognate promoter. false false _2538_ 31975 31975 9 false To be edited false iGEM UT-Tokyo 2016 component2525337 1 BBa_K2070013 component2525350 1 BBa_K2070004 annotation2525350 1 BBa_K2070004 range2525350 1 793 1756 annotation2525337 1 BBa_K2070013 range2525337 1 1 784 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K2070009 1 BBa_K2070009 ecf sigma35 2016-10-13T11:00:00Z 2016-10-14T06:15:23Z To be edited To be edited false false _2538_ 31975 31975 9 false To be edited false iGEM UT-Tokyo 2016 BBa_E0240 1 GFP report GFP generator 2004-10-17T11:00:00Z 2015-08-31T04:07:26Z Released HQ 2013 B0032.E0040.B0015 false true _11_1_ 0 61 7 In stock false true Jennifer Braff component1249221 1 BBa_B0010 component1249216 1 BBa_E0040 component1249213 1 BBa_B0032 component1249231 1 BBa_B0012 annotation1249216 1 BBa_E0040 range1249216 1 20 739 annotation1249231 1 BBa_B0012 range1249231 1 836 876 annotation1249213 1 BBa_B0032 range1249213 1 1 13 annotation1249221 1 BBa_B0010 range1249221 1 748 827 BBa_K2070013_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagtcacacaggaaagtactagatgcatgcgagcaccagcgcgaccaccgcggcgggcagcgatgaaaccctgctggcgcgctatcgcaaaggcgatagcgcggcgtttgaagtgctgtatcagcgccatcgccagggcctgtatcgctttctgtgcggcctggcgggccagaccgaactggcggatgaaatttatcaggaaacctggctgagcctgattcgcagcgaaagccagccgcagggccgcgcgagctttcgcacctggctgtatcagattgcgcgcaaccgcctgattgatcattggcgcaaacatggccagcgccagccgcttcaggatagctatgatgaacagctgcatgcgcagccggatcagggcgcgggcccggaacagcagctgagcctgagccgcgatggcgaacgccttcagagcgcgctggaaaccctgccggaagaacagcgcgaagtgtttctgatgcgcgcgcatggcgaactggaactggcggaaattgcgaccctgacccagagcccgctggaaaccgtgaaaagccgctttcgctatgcggtgcagaaactgcgccgcctgctggcggaagaagtggcggtgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K2070005_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagtcacacaggaaagtactagatgcatgcgagcaccagcgcgaccaccgcggcgggcagcgatgaaaccctgctggcgcgctatcgcaaaggcgatagcgcggcgtttgaagtgctgtatcagcgccatcgccagggcctgtatcgctttctgtgcggcctggcgggccagaccgaactggcggatgaaatttatcaggaaacctggctgagcctgattcgcagcgaaagccagccgcagggccgcgcgagctttcgcacctggctgtatcagattgcgcgcaaccgcctgattgatcattggcgcaaacatggccagcgccagccgcttcaggatagctatgatgaacagctgcatgcgcagccggatcagggcgcgggcccggaacagcagctgagcctgagccgcgatggcgaacgccttcagagcgcgctggaaaccctgccggaagaacagcgcgaagtgtttctgatgcgcgcgcatggcgaactggaactggcggaaattgcgaccctgacccagagcccgctggaaaccgtgaaaagccgctttcgctatgcggtgcagaaactgcgccgcctgctggcggaagaagtggcggtgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaccatccgtatttttttagggatttataaaacttttccatggcgtgcgtcgtctaataaatgggaaggaggaaatgatgttactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2070004_sequence 1 accatccgtatttttttagggatttataaaacttttccatggcgtgcgtcgtctaataaatgggaaggaggaaatgatgttactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0032_sequence 1 tcacacaggaaag BBa_E0240_sequence 1 tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2070009_sequence 1 atgcatgcgagcaccagcgcgaccaccgcggcgggcagcgatgaaaccctgctggcgcgctatcgcaaaggcgatagcgcggcgtttgaagtgctgtatcagcgccatcgccagggcctgtatcgctttctgtgcggcctggcgggccagaccgaactggcggatgaaatttatcaggaaacctggctgagcctgattcgcagcgaaagccagccgcagggccgcgcgagctttcgcacctggctgtatcagattgcgcgcaaccgcctgattgatcattggcgcaaacatggccagcgccagccgcttcaggatagctatgatgaacagctgcatgcgcagccggatcagggcgcgggcccggaacagcagctgagcctgagccgcgatggcgaacgccttcagagcgcgctggaaaccctgccggaagaacagcgcgaagtgtttctgatgcgcgcgcatggcgaactggaactggcggaaattgcgaccctgacccagagcccgctggaaaccgtgaaaagccgctttcgctatgcggtgcagaaactgcgccgcctgctggcggaagaagtggcggtgtaa BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2070008_sequence 1 accatccgtatttttttagggatttataaaacttttccatggcgtgcgtcgtctaataaatgggaaggaggaaatgatgt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z