LOCUS       BBa_K2070013             784 bp    DNA     linear       22-Nov-2024
DEFINITION  Constitutive ecf35 generator
ACCESSION   BBa_K2070013
VERSION     BBa_K2070013.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..647
                     /label=BBa_K2070009
     stem_loop       667..710
                     /label=stem_loop
     misc_feature    656..735
                     /label=BBa_B0010
     terminator      656..735
                     /label=BBa_B0010
     stem_loop       751..770
                     /label=T7 TE
     misc_feature    777..777
                     /label=stop
     polyA_site      771..784
                     /label=polya
     misc_feature    744..784
                     /label=BBa_B0012
     terminator      744..784
                     /label=BBa_B0012
     terminator      656..784
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatgcatgc gagcaccagc gcgaccaccg cggcgggcag cgatgaaacc ctgctggcgc
      121 gctatcgcaa aggcgatagc gcggcgtttg aagtgctgta tcagcgccat cgccagggcc
      181 tgtatcgctt tctgtgcggc ctggcgggcc agaccgaact ggcggatgaa atttatcagg
      241 aaacctggct gagcctgatt cgcagcgaaa gccagccgca gggccgcgcg agctttcgca
      301 cctggctgta tcagattgcg cgcaaccgcc tgattgatca ttggcgcaaa catggccagc
      361 gccagccgct tcaggatagc tatgatgaac agctgcatgc gcagccggat cagggcgcgg
      421 gcccggaaca gcagctgagc ctgagccgcg atggcgaacg ccttcagagc gcgctggaaa
      481 ccctgccgga agaacagcgc gaagtgtttc tgatgcgcgc gcatggcgaa ctggaactgg
      541 cggaaattgc gaccctgacc cagagcccgc tggaaaccgt gaaaagccgc tttcgctatg
      601 cggtgcagaa actgcgccgc ctgctggcgg aagaagtggc ggtgtaatac tagagccagg
      661 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      721 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      781 tata
//