LOCUS       BBa_K2070014             964 bp    DNA     linear       22-Nov-2024
DEFINITION  Constitutive antisigma35 generator
ACCESSION   BBa_K2070014
VERSION     BBa_K2070014.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     CDS             63..827
                     /label=BBa_K2070010
     stem_loop       847..890
                     /label=stem_loop
     misc_feature    836..915
                     /label=BBa_B0010
     terminator      836..915
                     /label=BBa_B0010
     stem_loop       931..950
                     /label=T7 TE
     misc_feature    957..957
                     /label=stop
     polyA_site      951..964
                     /label=polya
     misc_feature    924..964
                     /label=BBa_B0012
     terminator      924..964
                     /label=BBa_B0012
     terminator      836..964
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatgaaccc tcaaaagcac tctgcgtcag tggccgagga acaaatgctg gcgcacttca
      121 gagcgcatgc accgcagcaa cccgcgcctg cgttggacca ggctatatta gccgcagccc
      181 ggagacaggc tgcgcacgtt gaacccgctc gcagctggtg gcggcgttgg ttagaagcat
      241 cacgtcgccc gagatggcaa gcggccttcg catccctttt aggggtagcc ctggtcctgg
      301 gtctggtatc gcacaatgtc ctggacgacg cggaacgcca agctcggcct gaagttgcat
      361 ttagtgatgt accacttcgg gacggcgtcg ctggggctgc tgctgcaaaa cgggccatgc
      421 gtgccccagc ggcaccggcc ccactttctg gcgaaatgag tgagccaccg gctctgctgg
      481 cttcctacgc atcctcgggt gaagctccac agttgatggc ggaggctgct cctcccgccc
      541 ctgctgccct ggccgacaga ccgcccgccc aggcagccca gcaagccaag gtccaagccg
      601 ctttggcggg ggacttcgtg gcccaagcac ggggaaaagc ggtagctgtg aaacccgaag
      661 ttctggacga ggcgttaggc gcggttcttg cattacgcga acaaggcaaa actgaacaag
      721 cagccactca acttgccgaa cttcagaagc gttttcccgg agaaaactta gttgaacgcc
      781 tggagcgctt ggcaacaatt gcggcaagtg cgagaaaacg tccgtaatac tagagccagg
      841 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      901 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      961 tata
//