BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_J23101 1 BBa_J23101 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z later Released HQ 2013 later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K2070010 1 BBa_K2070010 ecf antisigma35 2016-10-13T11:00:00Z 2016-10-14T06:15:53Z To be edited To be edited false false _2538_ 31975 31975 9 false To be edited false iGEM UT-Tokyo 2016 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_K2070014 1 BBa_K2070014 Constitutive antisigma35 generator 2016-10-13T11:00:00Z 2016-10-20T05:27:33Z to be edited to be edited false false _2538_ 31975 31975 9 false to be edited false iGEM UT-Tokyo 2016 component2525201 1 BBa_K2070010 component2525208 1 BBa_B0015 component2525199 1 BBa_B0032 component2525197 1 BBa_J23101 annotation2525201 1 BBa_K2070010 range2525201 1 63 827 annotation2525199 1 BBa_B0032 range2525199 1 44 56 annotation2525208 1 BBa_B0015 range2525208 1 836 964 annotation2525197 1 BBa_J23101 range2525197 1 1 35 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K2070010_sequence 1 atgaaccctcaaaagcactctgcgtcagtggccgaggaacaaatgctggcgcacttcagagcgcatgcaccgcagcaacccgcgcctgcgttggaccaggctatattagccgcagcccggagacaggctgcgcacgttgaacccgctcgcagctggtggcggcgttggttagaagcatcacgtcgcccgagatggcaagcggccttcgcatcccttttaggggtagccctggtcctgggtctggtatcgcacaatgtcctggacgacgcggaacgccaagctcggcctgaagttgcatttagtgatgtaccacttcgggacggcgtcgctggggctgctgctgcaaaacgggccatgcgtgccccagcggcaccggccccactttctggcgaaatgagtgagccaccggctctgctggcttcctacgcatcctcgggtgaagctccacagttgatggcggaggctgctcctcccgcccctgctgccctggccgacagaccgcccgcccaggcagcccagcaagccaaggtccaagccgctttggcgggggacttcgtggcccaagcacggggaaaagcggtagctgtgaaacccgaagttctggacgaggcgttaggcgcggttcttgcattacgcgaacaaggcaaaactgaacaagcagccactcaacttgccgaacttcagaagcgttttcccggagaaaacttagttgaacgcctggagcgcttggcaacaattgcggcaagtgcgagaaaacgtccgtaa BBa_K2070014_sequence 1 tttacagctagctcagtcctaggtattatgctagctactagagtcacacaggaaagtactagatgaaccctcaaaagcactctgcgtcagtggccgaggaacaaatgctggcgcacttcagagcgcatgcaccgcagcaacccgcgcctgcgttggaccaggctatattagccgcagcccggagacaggctgcgcacgttgaacccgctcgcagctggtggcggcgttggttagaagcatcacgtcgcccgagatggcaagcggccttcgcatcccttttaggggtagccctggtcctgggtctggtatcgcacaatgtcctggacgacgcggaacgccaagctcggcctgaagttgcatttagtgatgtaccacttcgggacggcgtcgctggggctgctgctgcaaaacgggccatgcgtgccccagcggcaccggccccactttctggcgaaatgagtgagccaccggctctgctggcttcctacgcatcctcgggtgaagctccacagttgatggcggaggctgctcctcccgcccctgctgccctggccgacagaccgcccgcccaggcagcccagcaagccaaggtccaagccgctttggcgggggacttcgtggcccaagcacggggaaaagcggtagctgtgaaacccgaagttctggacgaggcgttaggcgcggttcttgcattacgcgaacaaggcaaaactgaacaagcagccactcaacttgccgaacttcagaagcgttttcccggagaaaacttagttgaacgcctggagcgcttggcaacaattgcggcaagtgcgagaaaacgtccgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0032_sequence 1 tcacacaggaaag BBa_J23101_sequence 1 tttacagctagctcagtcctaggtattatgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z