BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_J23101
1
BBa_J23101
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
later
Released HQ 2013
later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K2070001
1
ecf16
ecf16
2016-10-08T11:00:00Z
2016-10-20T05:45:54Z
Pseudomonas entomophila L48
ecf16 is a sigma factor, one of transcription factors, which activates ecf16 promoter (BBa_K2070000).
false
false
_2538_
31975
31975
9
false
For detail, see our wiki
false
iGEM UT-Tokyo 2016
BBa_K2070016
1
BBa_K2070016
Constitutive ecf16 generator
2016-10-08T11:00:00Z
2016-10-20T05:20:31Z
To be edited
To be edited
false
false
_2538_
31975
31975
9
false
To be edited
false
iGEM UT-Tokyo 2016
component2510083
1
BBa_B0015
component2510074
1
BBa_B0032
component2510076
1
BBa_K2070001
component2510072
1
BBa_J23101
annotation2510076
1
BBa_K2070001
range2510076
1
63
650
annotation2510072
1
BBa_J23101
range2510072
1
1
35
annotation2510074
1
BBa_B0032
range2510074
1
44
56
annotation2510083
1
BBa_B0015
range2510083
1
659
787
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1710
1
RBS
range1710
1
7
10
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K2070001_sequence
1
atgcagcgcaccaacagccaggatgtgctgagcacccgcgaaagccagcttcaggcgctgctgcttcagggcctggcgggcgatacctttgcgtatcgccagtttctgaccgcgctggcggcgcatattcgcggctttctgcgccgccgcctgccgcagcatccggcggaagtggaagatctgcttcaggaagtgctgctggcggtgcataacgcgcgccatacctatcaggcgcgccagccgctgaccgcgtgggtgcaggcgattgcgcgctataaactggcggatcatctgcgcagccatgcgcgccgcgaagcgcgccatgatctgctggatgatgatagcgaactgtttgcggcgagcgatgaacagccggcgcaggcgagccgcgatctgggcaaactgctgggccagctgccggatcgccagcgcctgccgattgtgcatgtgaaactggaaggcctgagcgtggaagaaaccgcgcagattaccggcctgagcagcagcgcggtgaaagtgggcattcatcgcggcctgaaagcgctgggcaaactgattcgcggcaaaggccatgatgaagatcgctaa
BBa_K2070016_sequence
1
tttacagctagctcagtcctaggtattatgctagctactagagtcacacaggaaagtactagatgcagcgcaccaacagccaggatgtgctgagcacccgcgaaagccagcttcaggcgctgctgcttcagggcctggcgggcgatacctttgcgtatcgccagtttctgaccgcgctggcggcgcatattcgcggctttctgcgccgccgcctgccgcagcatccggcggaagtggaagatctgcttcaggaagtgctgctggcggtgcataacgcgcgccatacctatcaggcgcgccagccgctgaccgcgtgggtgcaggcgattgcgcgctataaactggcggatcatctgcgcagccatgcgcgccgcgaagcgcgccatgatctgctggatgatgatagcgaactgtttgcggcgagcgatgaacagccggcgcaggcgagccgcgatctgggcaaactgctgggccagctgccggatcgccagcgcctgccgattgtgcatgtgaaactggaaggcctgagcgtggaagaaaccgcgcagattaccggcctgagcagcagcgcggtgaaagtgggcattcatcgcggcctgaaagcgctgggcaaactgattcgcggcaaaggccatgatgaagatcgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0032_sequence
1
tcacacaggaaag
BBa_J23101_sequence
1
tttacagctagctcagtcctaggtattatgctagc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z