LOCUS       BBa_K2070019             964 bp    DNA     linear       16-Jun-2024
DEFINITION  ecf sigma11 inducible GFP generator
ACCESSION   BBa_K2070019
VERSION     BBa_K2070019.1  
FEATURES             Location/Qualifiers
     promoter        1..80
                     /label=BBa_K1461002
     RBS             89..101
                     /label=RBS-3\Weak
     RBS             95..98
                     /label=RBS
     misc_feature    89..101
                     /label=BBa_B0032
     RBS             89..101
                     /label=BBa_B0032
     CDS             108..827
                     /label=GFP protein
     CDS             108..827
                     /label=BBa_E0040
     stem_loop       847..890
                     /label=stem_loop
     misc_feature    836..915
                     /label=BBa_B0010
     terminator      836..915
                     /label=BBa_B0010
     stem_loop       931..950
                     /label=T7 TE
     misc_feature    957..957
                     /label=stop
     polyA_site      951..964
                     /label=polya
     misc_feature    924..964
                     /label=BBa_B0012
     terminator      924..964
                     /label=BBa_B0012
     misc_feature    89..964
                     /label=BBa_E0240
ORIGIN
        1 gcctccacac cgctcgtcac atcctgtgat ccactcttca tcccgctacg taacacctct
       61 gcatcgcgaa ccaaaaccag tactagagtc acacaggaaa gtactagatg cgtaaaggag
      121 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
      181 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
      241 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttcg
      301 gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat gactttttca
      361 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
      421 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
      481 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
      541 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
      601 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
      661 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
      721 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
      781 cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac tagagccagg
      841 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      901 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
      961 tata
//