LOCUS       BBa_K2070020            1117 bp    DNA     linear       01-Jun-2024
DEFINITION  Cell division dependent GFP generator
ACCESSION   BBa_K2070020
VERSION     BBa_K2070020.1  
FEATURES             Location/Qualifiers
     promoter        1..233
                     /label=BBa_K847211
     RBS             242..254
                     /label=RBS-3\Weak
     RBS             248..251
                     /label=RBS
     misc_feature    242..254
                     /label=BBa_B0032
     RBS             242..254
                     /label=BBa_B0032
     CDS             261..980
                     /label=GFP protein
     CDS             261..980
                     /label=BBa_E0040
     stem_loop       1000..1043
                     /label=stem_loop
     misc_feature    989..1068
                     /label=BBa_B0010
     terminator      989..1068
                     /label=BBa_B0010
     stem_loop       1084..1103
                     /label=T7 TE
     misc_feature    1110..1110
                     /label=stop
     polyA_site      1104..1117
                     /label=polya
     misc_feature    1077..1117
                     /label=BBa_B0012
     terminator      1077..1117
                     /label=BBa_B0012
     misc_feature    242..1117
                     /label=BBa_E0240
ORIGIN
        1 aacctattat gccgttcaag aaatcgccga acagttattt ttaacaaatt tttctcttcc
       61 cattgacttt cccggacacc ttgtctgacc taaggtgcgc gaaagccact ttttccttcc
      121 tgagttatcc acaaagttat gcacttgcaa gagggtcatt ttcacactat cttgcagtga
      181 atcccaaaca taccccctat atatagtgtt ctaagcagct tcccgtacta cactactaga
      241 gtcacacagg aaagtactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      301 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      361 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      421 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      481 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      541 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      601 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      661 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      721 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      781 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      841 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      901 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      961 atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1021 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1081 actggctcac cttcgggtgg gcctttctgc gtttata
//