BBa_K2070026
type: ComponentDefinition
persistent identity: BBa K2070026
display id: BBa_K2070026
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive ecf16 generator with ecf 16 inducible GFP reporter
created: 2016-10-19T11:00:00Z
modified: 2016-10-20T05:43:06Z
mutable provenance: Assembly
owned by: james
owned by: myers
mutable description: This part was used to test the functionality of ECF sigma 16 and its cognate promoter
discontinued: false
dominant: false
group u list: _2538_
m user id: 31975
owner id: 31975
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: To be edited
star: false
creator: Rina Sakata
type: DnaRegion
role: partType/Project
role: SO:0000804
component: BBa_K2070016
component: BBa_K2070006
sequence annotation: BBa_K2070016
sequence annotation: BBa_K2070006
sequence: https://synbiohub.org/public/igem/BBa_K2070026_sequence/1

BBa_K2070016
type: Component
persistent identity: component2525285
display id: component2525285
version: 1
top level: BBa_K2070026
definition: BBa_K2070016
access: public

BBa_K2070006
type: Component
persistent identity: component2525298
display id: component2525298
version: 1
top level: BBa_K2070026
definition: BBa_K2070006
access: public

BBa_K2070016
type: SequenceAnnotation
persistent identity: annotation2525285
display id: annotation2525285
version: 1
top level: BBa_K2070026
location: https://synbiohub.org/public/igem/BBa_K2070026/annotation2525285/range2525285/1
component: BBa_K2070016

https://synbiohub.org/public/igem/BBa_K2070026/annotation2525285/range2525285/1
type: Range
persistent identity: range2525285
display id: range2525285
version: 1
top level: BBa_K2070026
direction: direction/reverse
start: 1
end: 787
orientation: inline

BBa_K2070006
type: SequenceAnnotation
persistent identity: annotation2525298
display id: annotation2525298
version: 1
top level: BBa_K2070026
location: https://synbiohub.org/public/igem/BBa_K2070026/annotation2525298/range2525298/1
component: BBa_K2070006

https://synbiohub.org/public/igem/BBa_K2070026/annotation2525298/range2525298/1
type: Range
persistent identity: range2525298
display id: range2525298
version: 1
top level: BBa_K2070026
direction: direction/reverse
start: 796
end: 1759
orientation: inline

BBa_K2070016
type: ComponentDefinition
persistent identity: BBa K2070016
display id: BBa_K2070016
version: 1
was generated by: iGEM to SBOL conversion
description: Constitutive ecf16 generator
created: 2016-10-08T11:00:00Z
modified: 2016-10-20T05:20:31Z
mutable provenance: To be edited
owned by: james
owned by: myers
mutable description: To be edited
discontinued: false
dominant: false
experience: experience/None
group u list: _2538_
m user id: 31975
owner id: 31975
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: To be edited
star: false
creator: iGEM UT-Tokyo 2016
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0015
component: BBa_K2070001
component: BBa_B0032
component: BBa_J23101
sequence annotation: BBa_J23101
sequence annotation: BBa_B0032
sequence annotation: BBa_K2070001
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_K2070016_sequence/1

BBa_B0015
type: Component
persistent identity: component2510083
display id: component2510083
version: 1
top level: BBa_K2070016
definition: BBa_B0015
access: public

BBa_K2070001
type: Component
persistent identity: component2510076
display id: component2510076
version: 1
top level: BBa_K2070016
definition: ecf16
access: public

BBa_B0032
type: Component
persistent identity: component2510074
display id: component2510074
version: 1
top level: BBa_K2070016
definition: BBa_B0032
access: public

BBa_J23101
type: Component
persistent identity: component2510072
display id: component2510072
version: 1
top level: BBa_K2070016
definition: BBa_J23101
access: public

BBa_J23101
type: SequenceAnnotation
persistent identity: annotation2510072
display id: annotation2510072
version: 1
top level: BBa_K2070016
location: https://synbiohub.org/public/igem/BBa_K2070016/annotation2510072/range2510072/1
component: BBa_J23101

https://synbiohub.org/public/igem/BBa_K2070016/annotation2510072/range2510072/1
type: Range
persistent identity: range2510072
display id: range2510072
version: 1
top level: BBa_K2070016
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation2510074
display id: annotation2510074
version: 1
top level: BBa_K2070016
location: https://synbiohub.org/public/igem/BBa_K2070016/annotation2510074/range2510074/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_K2070016/annotation2510074/range2510074/1
type: Range
persistent identity: range2510074
display id: range2510074
version: 1
top level: BBa_K2070016
direction: direction/forward
start: 44
end: 56
orientation: inline

BBa_K2070001
type: SequenceAnnotation
persistent identity: annotation2510076
display id: annotation2510076
version: 1
top level: BBa_K2070016
location: https://synbiohub.org/public/igem/BBa_K2070016/annotation2510076/range2510076/1
component: BBa_K2070001

https://synbiohub.org/public/igem/BBa_K2070016/annotation2510076/range2510076/1
type: Range
persistent identity: range2510076
display id: range2510076
version: 1
top level: BBa_K2070016
direction: direction/reverse
start: 63
end: 650
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2510083
display id: annotation2510083
version: 1
top level: BBa_K2070016
location: https://synbiohub.org/public/igem/BBa_K2070016/annotation2510083/range2510083/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K2070016/annotation2510083/range2510083/1
type: Range
persistent identity: range2510083
display id: range2510083
version: 1
top level: BBa_K2070016
direction: direction/reverse
start: 659
end: 787
orientation: inline

BBa_K2070006
type: ComponentDefinition
persistent identity: BBa K2070006
display id: BBa_K2070006
version: 1
was generated by: iGEM to SBOL conversion
description: ecf16 inducible GFP reporter
created: 2016-10-08T11:00:00Z
modified: 2016-10-20T05:11:36Z
mutable provenance: To be edited
owned by: james
owned by: myers
mutable description: to be edited
discontinued: false
dominant: false
experience: experience/None
group u list: _2538_
m user id: 31975
owner id: 31975
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: To be edited
star: false
creator: iGEM UT-Tokyo 2016
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K2070000
component: BBa_E0240
sequence annotation: BBa_K2070000
sequence annotation: BBa_E0240
sequence: https://synbiohub.org/public/igem/BBa_K2070006_sequence/1

BBa_K2070000
type: Component
persistent identity: component2525147
display id: component2525147
version: 1
top level: BBa_K2070006
definition: Psigma16
access: public

BBa_E0240
type: Component
persistent identity: component2525158
display id: component2525158
version: 1
top level: BBa_K2070006
definition: GFP report
access: public

BBa_K2070000
type: SequenceAnnotation
persistent identity: annotation2525147
display id: annotation2525147
version: 1
top level: BBa_K2070006
location: https://synbiohub.org/public/igem/BBa_K2070006/annotation2525147/range2525147/1
component: BBa_K2070000

https://synbiohub.org/public/igem/BBa_K2070006/annotation2525147/range2525147/1
type: Range
persistent identity: range2525147
display id: range2525147
version: 1
top level: BBa_K2070006
direction: direction/reverse
start: 1
end: 80
orientation: inline

BBa_E0240
type: SequenceAnnotation
persistent identity: annotation2525158
display id: annotation2525158
version: 1
top level: BBa_K2070006
location: https://synbiohub.org/public/igem/BBa_K2070006/annotation2525158/range2525158/1
component: BBa_E0240

https://synbiohub.org/public/igem/BBa_K2070006/annotation2525158/range2525158/1
type: Range
persistent identity: range2525158
display id: range2525158
version: 1
top level: BBa_K2070006
direction: direction/reverse
start: 89
end: 964
orientation: inline

BBa_B0032
type: ComponentDefinition
persistent identity: BBa B0032
display id: BBa_B0032
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.3 (medium) -- derivative of BBa_0030
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_44_48_46_1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: RBS-3\Weak
sequence annotation: BBa_B0032
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0032_sequence/1

RBS-3\Weak
type: SequenceAnnotation
persistent identity: annotation1709
display id: annotation1709
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1709/range1709/1
type: Range
persistent identity: range1709
display id: range1709
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation7027
display id: annotation7027
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0032/annotation7027/range7027/1
type: Range
persistent identity: range7027
display id: range7027
version: 1
top level: BBa_B0032
direction: direction/forward
start: 1
end: 13
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1710
display id: annotation1710
version: 1
top level: BBa_B0032
location: https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0032/annotation1710/range1710/1
type: Range
persistent identity: range1710
display id: range1710
version: 1
top level: BBa_B0032
direction: direction/forward
start: 7
end: 10
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

GFP report
type: ComponentDefinition
persistent identity: BBa E0240
display id: BBa_E0240
version: 1
was generated by: iGEM to SBOL conversion
description: GFP generator
created: 2004-10-17T11:00:00Z
modified: 2015-08-31T04:07:26Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: B0032.E0040.B0015
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_B0032
component: BBa_B0010
component: BBa_E0040
component: BBa_B0012
sequence annotation: BBa_B0032
sequence annotation: BBa_E0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_E0240_sequence/1

BBa_B0032
type: Component
persistent identity: component1249213
display id: component1249213
version: 1
top level: GFP report
definition: BBa_B0032
access: public

BBa_B0010
type: Component
persistent identity: component1249221
display id: component1249221
version: 1
top level: GFP report
definition: BBa_B0010
access: public

BBa_E0040
type: Component
persistent identity: component1249216
display id: component1249216
version: 1
top level: GFP report
definition: GFP
access: public

BBa_B0012
type: Component
persistent identity: component1249231
display id: component1249231
version: 1
top level: GFP report
definition: BBa_B0012
access: public

BBa_B0032
type: SequenceAnnotation
persistent identity: annotation1249213
display id: annotation1249213
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
component: BBa_B0032

https://synbiohub.org/public/igem/BBa_E0240/annotation1249213/range1249213/1
type: Range
persistent identity: range1249213
display id: range1249213
version: 1
top level: GFP report
direction: direction/forward
start: 1
end: 13
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation1249216
display id: annotation1249216
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_E0240/annotation1249216/range1249216/1
type: Range
persistent identity: range1249216
display id: range1249216
version: 1
top level: GFP report
direction: direction/forward
start: 20
end: 739
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1249221
display id: annotation1249221
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_E0240/annotation1249221/range1249221/1
type: Range
persistent identity: range1249221
display id: range1249221
version: 1
top level: GFP report
direction: direction/forward
start: 748
end: 827
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1249231
display id: annotation1249231
version: 1
top level: GFP report
location: https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_E0240/annotation1249231/range1249231/1
type: Range
persistent identity: range1249231
display id: range1249231
version: 1
top level: GFP report
direction: direction/forward
start: 836
end: 876
orientation: inline

BBa_J23101
type: ComponentDefinition
persistent identity: BBa J23101
display id: BBa_J23101
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-03T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: later
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_J23101_sequence/1

ecf16
type: ComponentDefinition
persistent identity: BBa K2070001
display id: BBa_K2070001
version: 1
was generated by: iGEM to SBOL conversion
description: ecf16
created: 2016-10-08T11:00:00Z
modified: 2016-10-20T05:45:54Z
mutable provenance: Pseudomonas entomophila L48
owned by: james
owned by: myers
mutable description: ecf16 is a sigma factor, one of transcription factors, which activates ecf16 promoter (BBa_K2070000).
discontinued: false
dominant: false
experience: experience/None
group u list: _2538_
m user id: 31975
owner id: 31975
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: For detail, see our wiki
star: false
creator: iGEM UT-Tokyo 2016
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K2070001_sequence/1

Psigma16
type: ComponentDefinition
persistent identity: BBa K2070000
display id: BBa_K2070000
version: 1
was generated by: iGEM to SBOL conversion
description: ecf16 promoter
created: 2016-10-08T11:00:00Z
modified: 2016-10-20T05:45:39Z
mutable provenance: Pseudomonas entomophila L48
owned by: james
owned by: myers
mutable description: ecf20 activates the expression of the downstream genes of this promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _2538_
m user id: 31975
owner id: 31975
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: For detail, see our wiki
star: false
creator: iGEM UT-Tokyo 2016
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_K2070000_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

https://synbiohub.org/public/igem/BBa_K2070026_sequence/1
type: Sequence
persistent identity: BBa K2070026 sequence
display id: BBa_K2070026_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagtcacacaggaaagtactagatgcagcgcaccaacagccaggatgtgctgagcacccgcgaaagccagcttcaggcgctgctgcttcagggcctggcgggcgatacctttgcgtatcgccagtttctgaccgcgctggcggcgcatattcgcggctttctgcgccgccgcctgccgcagcatccggcggaagtggaagatctgcttcaggaagtgctgctggcggtgcataacgcgcgccatacctatcaggcgcgccagccgctgaccgcgtgggtgcaggcgattgcgcgctataaactggcggatcatctgcgcagccatgcgcgccgcgaagcgcgccatgatctgctggatgatgatagcgaactgtttgcggcgagcgatgaacagccggcgcaggcgagccgcgatctgggcaaactgctgggccagctgccggatcgccagcgcctgccgattgtgcatgtgaaactggaaggcctgagcgtggaagaaaccgcgcagattaccggcctgagcagcagcgcggtgaaagtgggcattcatcgcggcctgaaagcgctgggcaaactgattcgcggcaaaggccatgatgaagatcgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcttggatgaaaagaaacccaccgacggtgtaaccctggcggccgatgcaacgaactaactcacaggacgtgctcagcacctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2070016_sequence/1
type: Sequence
persistent identity: BBa K2070016 sequence
display id: BBa_K2070016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagctactagagtcacacaggaaagtactagatgcagcgcaccaacagccaggatgtgctgagcacccgcgaaagccagcttcaggcgctgctgcttcagggcctggcgggcgatacctttgcgtatcgccagtttctgaccgcgctggcggcgcatattcgcggctttctgcgccgccgcctgccgcagcatccggcggaagtggaagatctgcttcaggaagtgctgctggcggtgcataacgcgcgccatacctatcaggcgcgccagccgctgaccgcgtgggtgcaggcgattgcgcgctataaactggcggatcatctgcgcagccatgcgcgccgcgaagcgcgccatgatctgctggatgatgatagcgaactgtttgcggcgagcgatgaacagccggcgcaggcgagccgcgatctgggcaaactgctgggccagctgccggatcgccagcgcctgccgattgtgcatgtgaaactggaaggcctgagcgtggaagaaaccgcgcagattaccggcctgagcagcagcgcggtgaaagtgggcattcatcgcggcctgaaagcgctgggcaaactgattcgcggcaaaggccatgatgaagatcgctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2070006_sequence/1
type: Sequence
persistent identity: BBa K2070006 sequence
display id: BBa_K2070006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttggatgaaaagaaacccaccgacggtgtaaccctggcggccgatgcaacgaactaactcacaggacgtgctcagcacctactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0032_sequence/1
type: Sequence
persistent identity: BBa B0032 sequence
display id: BBa_B0032_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0240_sequence/1
type: Sequence
persistent identity: BBa E0240 sequence
display id: BBa_E0240_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23101_sequence/1
type: Sequence
persistent identity: BBa J23101 sequence
display id: BBa_J23101_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacagctagctcagtcctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2070001_sequence/1
type: Sequence
persistent identity: BBa K2070001 sequence
display id: BBa_K2070001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcagcgcaccaacagccaggatgtgctgagcacccgcgaaagccagcttcaggcgctgctgcttcagggcctggcgggcgatacctttgcgtatcgccagtttctgaccgcgctggcggcgcatattcgcggctttctgcgccgccgcctgccgcagcatccggcggaagtggaagatctgcttcaggaagtgctgctggcggtgcataacgcgcgccatacctatcaggcgcgccagccgctgaccgcgtgggtgcaggcgattgcgcgctataaactggcggatcatctgcgcagccatgcgcgccgcgaagcgcgccatgatctgctggatgatgatagcgaactgtttgcggcgagcgatgaacagccggcgcaggcgagccgcgatctgggcaaactgctgggccagctgccggatcgccagcgcctgccgattgtgcatgtgaaactggaaggcctgagcgtggaagaaaccgcgcagattaccggcctgagcagcagcgcggtgaaagtgggcattcatcgcggcctgaaagcgctgggcaaactgattcgcggcaaaggccatgatgaagatcgctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K2070000_sequence/1
type: Sequence
persistent identity: BBa K2070000 sequence
display id: BBa_K2070000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: cttggatgaaaagaaacccaccgacggtgtaaccctggcggccgatgcaacgaactaactcacaggacgtgctcagcacc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z