LOCUS       BBa_K2070027             952 bp    DNA     linear       25-Nov-2024
DEFINITION  Cell cycle dependent GFPlva reporter
ACCESSION   BBa_K2070027
VERSION     BBa_K2070027.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    63..65
                     /label=start
     CDS             66..776
                     /label=GFP (mut3b)
     misc_feature    777..809
                     /label=LVA
     misc_feature    810..812
                     /label=stop
     misc_feature    813..815
                     /label=stop
     CDS             63..815
                     /label=BBa_K1399004
     stem_loop       835..878
                     /label=stem_loop
     misc_feature    824..903
                     /label=BBa_B0010
     terminator      824..903
                     /label=BBa_B0010
     stem_loop       919..938
                     /label=T7 TE
     misc_feature    945..945
                     /label=stop
     polyA_site      939..952
                     /label=polya
     misc_feature    912..952
                     /label=BBa_B0012
     terminator      912..952
                     /label=BBa_B0012
     terminator      824..952
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      121 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      181 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      241 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
      301 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      361 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
      421 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      481 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      541 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
      601 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
      661 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
      721 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaagctg
      781 caaacgacga aaactacgct ttagtagctt aataatacta gagccaggca tcaaataaaa
      841 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      901 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//