BBa_K2071969 1 BBa_K2071969 GLD1 is a general reductase, it is characterized in https://microbialcellfactories.biomedcentral.com 2016-10-13T11:00:00Z 2016-10-28T05:53:34Z This part was originally isolated from the genome of Trichoderma reesei as the gene GLD1. https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-016-0458-y This part codes for a reductase that can reduce erythrose to erythritol. It depends on NADPH as a redox co-factor. false false _2539_ 30728 30728 9 false We had two major design considerations when working with this sequence. Firstly we had to ensure that the sequence was codon optimized for eventual expression in Bacillus subtilis. We then also had to ensure that no RFC10 restriction sites were in the submitted sequence. We were able to correct both of these issues simultaneously using IDT???s codon optimization software. false Pavle Jeremic BBa_K2071969_sequence 1 atgagcagtggcagaacggtaactctcaatacgggctacaagattccacagataggatatggaacctggcaggcggctccaggagaggtgggcgcaggtgtctttgaggcacttaaagtgggctacagacatcttgatttggccaaggtttatggtaatcagaaagaagttggcgagggaatcaaaaaagcacttgcagaggttcctggattgaaacgggaggatatatttataacgagtaagctctggaataattctcacaagccggaggacgttgaaccagccttggatgatactctggccgaacttggattagattacttagacttatacctgatacattggccagtggccttcgcccctggtgccgacttattcccaaaatccgaggatggctcagaagttcaattaaatcagaatgtaagcattgtacaaacttggaaagcgatgacagagcttccgaagagcaaggtgcggagtgtcggagtatcaaatttcacaatcgaacaccttgatgccgtgattgaagcgacaggtgtggttcctgccgtgaatcagatagagcggcacccacgtttgccaaaccaacctcttatcgattactgtgcaaagaagggcatcatcataacggcctattcagcattcgggaacaataccaaagggctgcctcttttggtgtcttcagatgaggtaaaagctgtcgcagataatctgagtaaaaaacaaggcaaaactgtgacccctgcacaggtgattttagcgtggtcccaaatagggggccacacggtgattcctaagtccgtaacaaaagctcgcatcgcggaaaacttccaagaagttgaactcgacgatgaagctatcgcagcccttaacaaacttggtgagaagcctcagcgttttaacataccttacacatataagccgagatggaacattaatttgtttaacaccgaagaagagaaagcagccgctcacacagcagtgatcaaactttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z