LOCUS BBa_K2072000 3145 bp DNA linear 22-Nov-2024 DEFINITION pBAD-driven synthetic beta-glucosidase gene with araC ACCESSION BBa_K2072000 VERSION BBa_K2072000.1 FEATURES Location/Qualifiers misc_feature 1..113 /label=terminator BBa_J61048 CDS 114..1799 /label=truncated glycoside hydrolase from Microbacterium esteraromaticum strain GS514 codon optimzed for E.coli expression CDS 2138..3016 /label=araC promoter 1800..2137 /label=pBAD/araC promoter promoter 1800..2137 /label=pBAD/araC promoter misc_feature 3017..3145 /label=terminator BBa_B0015 ORIGIN 1 tttcttttgg gtatagcgtc gtggacagtc attcatcttt ctgcccctcc aaaagcaaaa 61 acccgccgaa gcgggttttt acgtaaatca ggtgaaactg accgataagc cggttatcag 121 ccgcccgtag tgtaagataa cccatgtccg aatggataca gaacgtcctg gtctcttgca 181 tcgtaacccc tataaccgac aaagacacct tcgccatacc ggacacggct atgttcacca 241 gggaagtgca gataactggg cgcgtcttgc agacgcagag gtacagtttc agccagacgc 301 cctgaagggt cgaccttccc gaacaggaca tccgcaatgg cgctgccact cgcctggccc 361 agaaggccgc cgttcaagat cgctgttgct gtagcggcca ccggggctaa ccgcagcaca 421 cccccggaag ccaggaccac aactgtatgc ggttgcacgg cggcaattgc ctccaacaat 481 gccaattgct cagcaggaag ttctatatct tcgcgatcaa acccttcgct ttcctgatgt 541 tcggcaaggc cgagaaataa aacagcaaca tccgccgcat ctgcaatctc aacagcatcc 601 gcatgaagag cgtcttggtt acctgttcca ttcactgtaa aaccggccgc gtgagtcaca 661 tgagcgttgg gtgccgccgc gcgaatttga tccagtggga tgtccaggcg agttgggcga 721 acatggcttg acccaccacc ctggtatcgt ggctcagtcg caaaactacc gatcactgca 781 atacgtgccg ttggatcgag tggcagcaag gcggattcgt ttttcaggag cacgatcgat 841 ctccccgccg tctcacgtgc gagtgcatga tgggcgtcat aatctactgt tacttcgcgt 901 cggctccgct gcgcaaggag ggcggtacgt gcaactgccc gggccgcacg ggtgactgct 961 gcgcggggta aggtaccagc ttcaacagcc gcaactaccg cggcgtcaaa agcggatgta 1021 tcaccgggca tctggagatc aagccctgca tcgactgcgg caactctatc ggccacggcg 1081 ccccagtcag aaaccacagc gccggcaaag ccccattgat cacgcagaac gtcagttaac 1141 aaccagcgtg actgagatgc taaaactccg ttaattctat tataagagca catgacggtc 1201 cacggttggg catcgcgaat tactgtctcg aaagcacgca agtagatttc gtgtagaggc 1261 ctttcatcca cgtcgctgga aagccgcatc ctatcaaact cagcattgtt cgcggcaaag 1321 tgttttaacg aggctcctac cccaccgctc tgaaggccgt tcacccacga cgtggccagg 1381 actcccgtaa gggccgggtc ttcagagagg tattcaaaat tacgtccgca gcgtggatca 1441 cgtttgatat taatacccgg tcctaacaga acattgactc cttgtgcatg cgcttcttcg 1501 gccagggcag ccgccaccct tcgcgctagg gccgggtccc aactttgacc catgctcaca 1561 gcgggaggaa agcaagtggc gggcacactc gcgcccaaac ccagatgatc tgctgcatca 1621 ccttcctgtt tccttaggcc atgcggacca tccgacatgc taaaactggg aaccccaggc 1681 gccgttttac tctcccaaaa actcgcgcct ccaccaagag ccgccagttg cgtcaggttc 1741 aatctctcaa tagccgcgtc cagggctcct tctattggat ttgtcatttg aggttccatc 1801 gtttcactcc atccaaaaaa acgggtatgg agaaacagta gagagttgcg ataaaaagcg 1861 tcaggtagga tccgctaatc ttatggataa aaatgctatg gcatagcaaa gtgtgacgcc 1921 gtgcaaataa tcaatgtgga cttttctgcc gtgattatag acacttttgt tacgcgtttt 1981 tgtcatggct ttggtcccgc tttgttacag aatgctttta ataagcgggg ttaccggttg 2041 ggttagcgag aagagccagt aaaagacgca gtgacggcaa tgtctgatgc aatatggaca 2101 attggtttct tctctgaatg gtgggagtat gaaaagtatg gctgaagcgc aaaatgatcc 2161 cctgctgccg ggatactcgt ttaacgccca tctggtggcg ggtttaacgc cgattgaggc 2221 caacggttat ctcgattttt ttatcgaccg accgctggga atgaaaggtt atattctcaa 2281 tctcaccatt cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg tctgccgacc 2341 gggtgatatt ttgctgttcc cgccaggaga gattcatcac tacggtcgtc atccggaggc 2401 tcgcgaatgg tatcaccagt gggtttactt tcgtccgcgc gcctactggc atgaatggct 2461 taactggccg tcaatatttg ccaatacggg tttctttcgc ccggatgaag cgcaccagcc 2521 gcatttcagc gacctgtttg ggcaaatcat taacgccggg caaggggaag ggcgctattc 2581 ggagctgctg gcgataaatc tgcttgagca attgttactg cggcgcatgg aagcgattaa 2641 cgagtcgctc catccaccga tggataatcg ggtacgcgag gcttgtcagt acatcagcga 2701 tcacctggca gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt gcttgtcgcc 2761 gtcgcgtctg tcacatcttt tccgccagca gttagggatt agcgtcttaa gctggcgcga 2821 ggaccaacgc attagtcagg cgaagctgct tttgagcact acccggatgc ctatcgccac 2881 cgtcggtcgc aatgttggtt ttgacgatca actctatttc tcgcgagtat ttaaaaaatg 2941 caccggggcc agcccgagcg agtttcgtgc cggttgtgaa gaaaaagtga atgatgtagc 3001 cgtcaagttg tcataaccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 3061 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 3121 tcgggtgggc ctttctgcgt ttata //