LOCUS       BBa_K2073006            1913 bp    DNA     linear       13-Jun-2024
DEFINITION  kit1 fac-dex1+GFP
ACCESSION   BBa_K2073006
VERSION     BBa_K2073006.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1138
                     /label=BBa_K2073000
     CDS             1145..1864
                     /label=GFP protein
     CDS             1145..1864
                     /label=BBa_E0040
     stem_loop       1880..1899
                     /label=T7 TE
     misc_feature    1906..1906
                     /label=stop
     polyA_site      1900..1913
                     /label=polya
     misc_feature    1873..1913
                     /label=BBa_B0012
     terminator      1873..1913
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt tcgaaggttt
      241 cgaacgtcgt ctggttgacg ttggtgacgt taccatcaac tgcgttgttg gtggttctgg
      301 tccggctctg ctgctcctcc acggcttccc gcagaacctc cacatgtggg ctcgtgttgc
      361 tccgctgctg gctaacgaat acaccgttgt ttgcgctgac ctgcgtggtt acggtggttc
      421 ttctaaaccg gttggtgctc cggaccacgc taactactct ttccgtgcta tggcttctga
      481 ccagcgtgaa ctgatgcgta ccctgggttt cgaacgtttc cacctggttg gtcacgaccg
      541 tggtggtcgt accggtcacc gtatggctct ggaccacccg gactctgttc tgtctctggc
      601 tgttctggac atcatcccga cctacgttat gttcgaagaa gttgaccgtt tcgttgctcg
      661 tgcttactgg cactggtact tcctgcaaca gccggctccg tacccggaaa aagttatcgg
      721 tgctgacccg gacaccttct acgaaggttg cctgttcggt tggggtgcta ccggtgctga
      781 cggtttcgac ccggaacagc tggaagaata ccgtaaacag tggcgtgacc cggctgctat
      841 ccacggttct tgctgcgact accgtgctgg tggtaccatc gacttcgaac tggaccacgg
      901 tgacctgggt cgtcaggttc agtgtccagc tctggtattc agcggttctg ctggtctgat
      961 gcactctctg ttcgaaatgc aggttgtttg ggctccgcgt ctggctaaca tgcgtttcgc
     1021 ttctctgccg ggtggtcact tcttcgttga ccgtttcccg gacgacaccg ctcgtatcct
     1081 gcgtgaattt ctgtctgacg ctcgttctgg tatccaccag accgaacgtc gtgaatctta
     1141 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
     1201 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
     1261 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
     1321 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
     1381 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
     1441 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
     1501 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
     1561 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
     1621 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
     1681 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
     1741 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
     1801 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
     1861 ataatactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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