LOCUS       BBa_K2073007            2072 bp    DNA     linear       01-Jun-2024
DEFINITION  kit-3 facdex3+GFP
ACCESSION   BBa_K2073007
VERSION     BBa_K2073007.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1297
                     /label=BBa_K2073001
     CDS             1304..2023
                     /label=GFP protein
     CDS             1304..2023
                     /label=BBa_E0040
     stem_loop       2039..2058
                     /label=T7 TE
     misc_feature    2065..2065
                     /label=stop
     polyA_site      2059..2072
                     /label=polya
     misc_feature    2032..2072
                     /label=BBa_B0012
     terminator      2032..2072
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg acaccaccct
      241 gaaccacccg tctgctctgc gtcgtacccg tctgttcacc ccggcgatac tggtattcgc
      301 aatcatgcag ctgggtgctc tgggtttcgc ttcttctcag gctaacgctg ctgacgttcc
      361 ggcttctacc atcggtgcta tcaccccggg ttctcacacc tctttcggtc cgctgaaaca
      421 cgttaaagct ggcctcctgg acgtgagcta cgctgaagtt ggtccggctg acggtccggt
      481 tgttatcctg ctgcacggtt ggccgtacga catccactct tacgctgacg ttgctccggc
      541 tctggctgaa aaaggttacc gtgttctgat cccgtacgct cgtggttacg gtgacacccg
      601 tttcctgtct gctaaaaccc tgcgtaacgg tcagccgtct gctctggcta acgacctgat
      661 cgacttcatg gacgctctga aaatcaaaca ggctgttctg ggtggttacg actggggtgc
      721 tcgtaccgct gacatcgttg ctgctctgtg gccggaacgt gttaaagctc tggttgctgt
      781 ttctggttac ctgatcggtt ctcaggaagc tggtaaaacc ccgctgccgc cggctgctga
      841 actgcaatgg tggtaccagt tctacttcgc taccgaacgt ggtcgtctgg gttacgaaaa
      901 aaacacccac gacttcgcta aactgatctg gcagctggct tctccgaaat ggaacttcga
      961 cgacgctacc tacgaccgtt ctgctgctgc tctgcaaaac ccggaccacg ttgctgtttc
     1021 tatcttcaac taccgttggc gtctgggtct ggttaaaggt gaagctaaat acgacgctct
     1081 ggaaaaaaaa ctggctacct tcccgtctat ctctgttccg accatcaccc tggaaggtga
     1141 cgctaacggt gctccgcacc cgccggctga agcttacgct aaacgtttca ccggtcagta
     1201 cgaacaccgt ctgatcgacg gtggtgttgg tcacaacctg ccgcaggaag cgccacaggc
     1261 gttcgctcag gctgttatcg acgctgacca cctgcgttac tagatgcgta aaggagaaga
     1321 acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa
     1381 attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt
     1441 tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta ctttcggtta
     1501 tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact ttttcaagag
     1561 tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg acgggaacta
     1621 caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
     1681 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
     1741 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa
     1801 aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc aacaaaatac
     1861 tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca cacaatctgc
     1921 cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt ttgtaacagc
     1981 tgctgggatt acacatggca tggatgaact atacaaataa taatactaga gtcacactgg
     2041 ctcaccttcg ggtgggcctt tctgcgttta ta
//