BBa_J04500 1 BBa_J04500 IPTG inducible promoter with RBS 2005-06-08T11:00:00Z 2015-08-31T04:08:14Z Davidson Synth-Aces Released HQ 2013 R0010.B0034 false true _16_ 0 326 16 In stock false false Kristen DeCelle component1508159 1 BBa_B0034 component1508149 1 BBa_R0010 annotation1508159 1 BBa_B0034 range1508159 1 209 220 annotation1508149 1 BBa_R0010 range1508149 1 1 200 BBa_K2073007 1 BBa_K2073007 kit-3 facdex3+GFP 2016-10-13T11:00:00Z 2016-10-14T06:02:52Z BBa_J04500+BBa_K2073001+BBa_E0040+BBa_B0012 this part contain BBa_J04500(IPTG promoter+RBS)+BBa_K2073001(fac-dex3)+BBa_E0040(GFP)+BBa_B0012(ter) We transform this biobrick into E.coli strain BL21, and use IPTG promoter to control the fac-dex1 protein express. When this engineered bacteria be induced by IPTG overnight (at least 12 hours), it would show GFP fluorescence. Then we know the fac-dex1 is expressed by bacteria. false false _2541_ 30741 30741 9 false If bacteria show GFP fluorescence, we could know the fac-dex3 is successfully expressed false YU-CHUN LIN component2502631 1 BBa_B0012 component2502628 1 BBa_K2073001 component2502627 1 BBa_J04500 component2502630 1 BBa_E0040 annotation2502627 1 BBa_J04500 range2502627 1 1 220 annotation2502628 1 BBa_K2073001 range2502628 1 227 1297 annotation2502631 1 BBa_B0012 range2502631 1 2032 2072 annotation2502630 1 BBa_E0040 range2502630 1 1304 2023 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961224 1 -35 range1961224 1 137 142 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961225 1 -10 range1961225 1 161 166 annotation1961227 1 start range1961227 1 173 173 annotation1961222 1 BBa_R0010 range1961222 1 1 200 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K2073001 1 BBa_K2073001 fac-dex3 2016-10-11T11:00:00Z 2016-10-29T04:34:37Z SOURCE Pseudomonas fluorescens ORGANISM Pseudomonas fluorescens Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. fac-dex3 is a kind of fluoroacetate dehalogenase. the enzyme can cleavage of a carbonfluorine bond in fluoroacetate to form glycolate. The reaction: a haloacetate + H2O &#8652; glycolate + a halide anion + H+ The enzyme ,fac-dex3 belongs to Haloalkane Dehalogenases family, which catalyzes the hydrolytic cleavage of carbon- halogen bonds. Sequence and Features false false _2541_ 30752 30741 9 false we use this enzyme to break down PFOA false WEI-CHIA HSU BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_J04500_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2073001_sequence 1 atggacaccaccctgaaccacccgtctgctctgcgtcgtacccgtctgttcaccccggcgatactggtattcgcaatcatgcagctgggtgctctgggtttcgcttcttctcaggctaacgctgctgacgttccggcttctaccatcggtgctatcaccccgggttctcacacctctttcggtccgctgaaacacgttaaagctggcctcctggacgtgagctacgctgaagttggtccggctgacggtccggttgttatcctgctgcacggttggccgtacgacatccactcttacgctgacgttgctccggctctggctgaaaaaggttaccgtgttctgatcccgtacgctcgtggttacggtgacacccgtttcctgtctgctaaaaccctgcgtaacggtcagccgtctgctctggctaacgacctgatcgacttcatggacgctctgaaaatcaaacaggctgttctgggtggttacgactggggtgctcgtaccgctgacatcgttgctgctctgtggccggaacgtgttaaagctctggttgctgtttctggttacctgatcggttctcaggaagctggtaaaaccccgctgccgccggctgctgaactgcaatggtggtaccagttctacttcgctaccgaacgtggtcgtctgggttacgaaaaaaacacccacgacttcgctaaactgatctggcagctggcttctccgaaatggaacttcgacgacgctacctacgaccgttctgctgctgctctgcaaaacccggaccacgttgctgtttctatcttcaactaccgttggcgtctgggtctggttaaaggtgaagctaaatacgacgctctggaaaaaaaactggctaccttcccgtctatctctgttccgaccatcaccctggaaggtgacgctaacggtgctccgcacccgccggctgaagcttacgctaaacgtttcaccggtcagtacgaacaccgtctgatcgacggtggtgttggtcacaacctgccgcaggaagcgccacaggcgttcgctcaggctgttatcgacgctgaccacctgcgt BBa_K2073007_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggacaccaccctgaaccacccgtctgctctgcgtcgtacccgtctgttcaccccggcgatactggtattcgcaatcatgcagctgggtgctctgggtttcgcttcttctcaggctaacgctgctgacgttccggcttctaccatcggtgctatcaccccgggttctcacacctctttcggtccgctgaaacacgttaaagctggcctcctggacgtgagctacgctgaagttggtccggctgacggtccggttgttatcctgctgcacggttggccgtacgacatccactcttacgctgacgttgctccggctctggctgaaaaaggttaccgtgttctgatcccgtacgctcgtggttacggtgacacccgtttcctgtctgctaaaaccctgcgtaacggtcagccgtctgctctggctaacgacctgatcgacttcatggacgctctgaaaatcaaacaggctgttctgggtggttacgactggggtgctcgtaccgctgacatcgttgctgctctgtggccggaacgtgttaaagctctggttgctgtttctggttacctgatcggttctcaggaagctggtaaaaccccgctgccgccggctgctgaactgcaatggtggtaccagttctacttcgctaccgaacgtggtcgtctgggttacgaaaaaaacacccacgacttcgctaaactgatctggcagctggcttctccgaaatggaacttcgacgacgctacctacgaccgttctgctgctgctctgcaaaacccggaccacgttgctgtttctatcttcaactaccgttggcgtctgggtctggttaaaggtgaagctaaatacgacgctctggaaaaaaaactggctaccttcccgtctatctctgttccgaccatcaccctggaaggtgacgctaacggtgctccgcacccgccggctgaagcttacgctaaacgtttcaccggtcagtacgaacaccgtctgatcgacggtggtgttggtcacaacctgccgcaggaagcgccacaggcgttcgctcaggctgttatcgacgctgaccacctgcgttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z