LOCUS       BBa_K2073008            1187 bp    DNA     linear       25-Nov-2024
DEFINITION  fac-dex1 with IPTG promoter and ter
ACCESSION   BBa_K2073008
VERSION     BBa_K2073008.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..1138
                     /label=BBa_K2073000
     stem_loop       1154..1173
                     /label=T7 TE
     misc_feature    1180..1180
                     /label=stop
     polyA_site      1174..1187
                     /label=polya
     misc_feature    1147..1187
                     /label=BBa_B0012
     terminator      1147..1187
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgt tcgaaggttt
      241 cgaacgtcgt ctggttgacg ttggtgacgt taccatcaac tgcgttgttg gtggttctgg
      301 tccggctctg ctgctcctcc acggcttccc gcagaacctc cacatgtggg ctcgtgttgc
      361 tccgctgctg gctaacgaat acaccgttgt ttgcgctgac ctgcgtggtt acggtggttc
      421 ttctaaaccg gttggtgctc cggaccacgc taactactct ttccgtgcta tggcttctga
      481 ccagcgtgaa ctgatgcgta ccctgggttt cgaacgtttc cacctggttg gtcacgaccg
      541 tggtggtcgt accggtcacc gtatggctct ggaccacccg gactctgttc tgtctctggc
      601 tgttctggac atcatcccga cctacgttat gttcgaagaa gttgaccgtt tcgttgctcg
      661 tgcttactgg cactggtact tcctgcaaca gccggctccg tacccggaaa aagttatcgg
      721 tgctgacccg gacaccttct acgaaggttg cctgttcggt tggggtgcta ccggtgctga
      781 cggtttcgac ccggaacagc tggaagaata ccgtaaacag tggcgtgacc cggctgctat
      841 ccacggttct tgctgcgact accgtgctgg tggtaccatc gacttcgaac tggaccacgg
      901 tgacctgggt cgtcaggttc agtgtccagc tctggtattc agcggttctg ctggtctgat
      961 gcactctctg ttcgaaatgc aggttgtttg ggctccgcgt ctggctaaca tgcgtttcgc
     1021 ttctctgccg ggtggtcact tcttcgttga ccgtttcccg gacgacaccg ctcgtatcct
     1081 gcgtgaattt ctgtctgacg ctcgttctgg tatccaccag accgaacgtc gtgaatctta
     1141 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//