BBa_J04500 1 BBa_J04500 IPTG inducible promoter with RBS 2005-06-08T11:00:00Z 2015-08-31T04:08:14Z Davidson Synth-Aces Released HQ 2013 R0010.B0034 false true _16_ 0 326 16 In stock false false Kristen DeCelle component1508159 1 BBa_B0034 component1508149 1 BBa_R0010 annotation1508159 1 BBa_B0034 range1508159 1 209 220 annotation1508149 1 BBa_R0010 range1508149 1 1 200 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961224 1 -35 range1961224 1 137 142 annotation1961227 1 start range1961227 1 173 173 annotation1961225 1 -10 range1961225 1 161 166 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K2073001 1 BBa_K2073001 fac-dex3 2016-10-11T11:00:00Z 2016-10-29T04:34:37Z SOURCE Pseudomonas fluorescens ORGANISM Pseudomonas fluorescens Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. fac-dex3 is a kind of fluoroacetate dehalogenase. the enzyme can cleavage of a carbonfluorine bond in fluoroacetate to form glycolate. The reaction: a haloacetate + H2O &#8652; glycolate + a halide anion + H+ The enzyme ,fac-dex3 belongs to Haloalkane Dehalogenases family, which catalyzes the hydrolytic cleavage of carbon- halogen bonds. Sequence and Features false false _2541_ 30752 30741 9 false we use this enzyme to break down PFOA false WEI-CHIA HSU BBa_K2073009 1 BBa_K2073009 fac-dex3 with IPTG promoter 2016-10-13T11:00:00Z 2016-10-29T04:35:06Z BBa_J04500(IPTG promoter+RBS)+BBa_K2073001(fac-dex3)+BBa_B0012(ter) This part contain BBa_J04500(IPTG promoter+RBS)+BBa_K2073001(fac-dex3)+BBa_B0012(ter). This biobrick can be transform into E.coli, then the E.coli can express fac-dex3. fac-dex3 is a kind of fluoroacetate dehalogenase. The enzyme can cleavage of a carbonfluorine bond in fluoroacetate to form glycolate. The reaction: a haloacetate + H2O &#8652; glycolate + a halide anion + H+ false false _2541_ 30752 30741 9 false we can use IPTG to induce engineered bacteria expressing fac-dex1. false WEI-CHIA HSU component2502771 1 BBa_J04500 component2502773 1 BBa_B0012 component2502772 1 BBa_K2073001 annotation2502773 1 BBa_B0012 range2502773 1 1306 1346 annotation2502772 1 BBa_K2073001 range2502772 1 227 1297 annotation2502771 1 BBa_J04500 range2502771 1 1 220 BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_J04500_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2073009_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggacaccaccctgaaccacccgtctgctctgcgtcgtacccgtctgttcaccccggcgatactggtattcgcaatcatgcagctgggtgctctgggtttcgcttcttctcaggctaacgctgctgacgttccggcttctaccatcggtgctatcaccccgggttctcacacctctttcggtccgctgaaacacgttaaagctggcctcctggacgtgagctacgctgaagttggtccggctgacggtccggttgttatcctgctgcacggttggccgtacgacatccactcttacgctgacgttgctccggctctggctgaaaaaggttaccgtgttctgatcccgtacgctcgtggttacggtgacacccgtttcctgtctgctaaaaccctgcgtaacggtcagccgtctgctctggctaacgacctgatcgacttcatggacgctctgaaaatcaaacaggctgttctgggtggttacgactggggtgctcgtaccgctgacatcgttgctgctctgtggccggaacgtgttaaagctctggttgctgtttctggttacctgatcggttctcaggaagctggtaaaaccccgctgccgccggctgctgaactgcaatggtggtaccagttctacttcgctaccgaacgtggtcgtctgggttacgaaaaaaacacccacgacttcgctaaactgatctggcagctggcttctccgaaatggaacttcgacgacgctacctacgaccgttctgctgctgctctgcaaaacccggaccacgttgctgtttctatcttcaactaccgttggcgtctgggtctggttaaaggtgaagctaaatacgacgctctggaaaaaaaactggctaccttcccgtctatctctgttccgaccatcaccctggaaggtgacgctaacggtgctccgcacccgccggctgaagcttacgctaaacgtttcaccggtcagtacgaacaccgtctgatcgacggtggtgttggtcacaacctgccgcaggaagcgccacaggcgttcgctcaggctgttatcgacgctgaccacctgcgttactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K2073001_sequence 1 atggacaccaccctgaaccacccgtctgctctgcgtcgtacccgtctgttcaccccggcgatactggtattcgcaatcatgcagctgggtgctctgggtttcgcttcttctcaggctaacgctgctgacgttccggcttctaccatcggtgctatcaccccgggttctcacacctctttcggtccgctgaaacacgttaaagctggcctcctggacgtgagctacgctgaagttggtccggctgacggtccggttgttatcctgctgcacggttggccgtacgacatccactcttacgctgacgttgctccggctctggctgaaaaaggttaccgtgttctgatcccgtacgctcgtggttacggtgacacccgtttcctgtctgctaaaaccctgcgtaacggtcagccgtctgctctggctaacgacctgatcgacttcatggacgctctgaaaatcaaacaggctgttctgggtggttacgactggggtgctcgtaccgctgacatcgttgctgctctgtggccggaacgtgttaaagctctggttgctgtttctggttacctgatcggttctcaggaagctggtaaaaccccgctgccgccggctgctgaactgcaatggtggtaccagttctacttcgctaccgaacgtggtcgtctgggttacgaaaaaaacacccacgacttcgctaaactgatctggcagctggcttctccgaaatggaacttcgacgacgctacctacgaccgttctgctgctgctctgcaaaacccggaccacgttgctgtttctatcttcaactaccgttggcgtctgggtctggttaaaggtgaagctaaatacgacgctctggaaaaaaaactggctaccttcccgtctatctctgttccgaccatcaccctggaaggtgacgctaacggtgctccgcacccgccggctgaagcttacgctaaacgtttcaccggtcagtacgaacaccgtctgatcgacggtggtgttggtcacaacctgccgcaggaagcgccacaggcgttcgctcaggctgttatcgacgctgaccacctgcgt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z