LOCUS       BBa_K2073010             656 bp    DNA     linear       29-Nov-2024
DEFINITION  Crcb(flc) with IPTG promoter
ACCESSION   BBa_K2073010
VERSION     BBa_K2073010.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..607
                     /label=BBa_K2073002
     stem_loop       623..642
                     /label=T7 TE
     misc_feature    649..649
                     /label=stop
     polyA_site      643..656
                     /label=polya
     misc_feature    616..656
                     /label=BBa_B0012
     terminator      616..656
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc tgcaactcct
      241 cctggctgta ttcatcgggg gtggtaccgg ttctgttgct cgttggctgc tgtctatgcg
      301 tttcaacccg ctgcaccagg ctatcccgct gggtaccctg accgctaacc tgatcggtgc
      361 tttcatcatc ggtatcggtt tcgcttggtt ctctcgtatg accaacatcg acccggtttg
      421 gaaagttctg atcaccaccg gtttctgcgg tggtctgacc accttctcta ccttctctgc
      481 ggaggttgtc ttcctgctgc aagagggcag gttcggttgg gcgctcctga acgtgttcgt
      541 aaacctgctg ggttctttcg ctatgaccgc tctggctttc tggctgttct ctgcttctac
      601 cgctcactac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//