LOCUS       BBa_K2073011             659 bp    DNA     linear       23-Dec-2024
DEFINITION  CRCB(bpe) with IPTG promoter
ACCESSION   BBa_K2073011
VERSION     BBa_K2073011.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..610
                     /label=BBa_K2073003
     stem_loop       626..645
                     /label=T7 TE
     misc_feature    652..652
                     /label=stop
     polyA_site      646..659
                     /label=polya
     misc_feature    619..659
                     /label=BBa_B0012
     terminator      619..659
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc tgacctacgc
      241 tccgctgaac ttcatcgcta tcggtatcgg tgctaccctg ggtgcttggc tgcgttgggt
      301 tctgggtctg cgtctgaacg gtgctggttg gccgtggggt accctgaccg ctaacctggt
      361 tggtggttac ctgatcggtg ttatggttgc tctgatcgct tctcacccgg aatggccggc
      421 ttggatccgt ctggctgctg ttaccggttt cctgggtggt ctgaccacct tctctacctt
      481 ctctgctgaa accgttgaca tgctggaacg tggtgtttac gctaccgctg ctgcttacgc
      541 tggtgcttct ctggctggtt ctctggctat gaccggtctg ggtctggcta ccgttcgtct
      601 gctgctgcgt tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//