LOCUS       BBa_K2073012             662 bp    DNA     linear       13-Jun-2024
DEFINITION  CrcB-La1 with IPTG promoter
ACCESSION   BBa_K2073012
VERSION     BBa_K2073012.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..613
                     /label=BBa_K2073004
     stem_loop       629..648
                     /label=T7 TE
     misc_feature    655..655
                     /label=stop
     polyA_site      649..662
                     /label=polya
     misc_feature    622..662
                     /label=BBa_B0012
     terminator      622..662
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga accgtcgtct
      241 gaaaaactac ctgtctgttg gtatcttcgc tttcttcggt ggtggtctgc gtgcttacct
      301 gaacctgatc tggtctcaga ccggtaccct gaccgctaac atcatcggtt gcttcctgct
      361 ggctttcttc acctacttct tcgttgaata ccgtgaaggt cgtgactggc tggttaccgg
      421 tctgtcgaca ggcttcgttg gttcttttac caccttctct tctttcaacc tggacaccct
      481 gaaacagctg gaatctggta tgaactctca ggctaccatc tacttcttct cttctatctt
      541 catcggtttc ctgttcgctt acctgggtat gctggttggt aaacgtaccg gtcgtaaact
      601 ggctgaaaaa gcttactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      661 ta
//