LOCUS       BBa_K2073013             632 bp    DNA     linear       01-Jan-2026
DEFINITION  CrcB-La2 with IPTG promoter
ACCESSION   BBa_K2073013
VERSION     BBa_K2073013.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..583
                     /label=BBa_K2073005
     stem_loop       599..618
                     /label=T7 TE
     misc_feature    625..625
                     /label=stop
     polyA_site      619..632
                     /label=polya
     misc_feature    592..632
                     /label=BBa_B0012
     terminator      592..632
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga tcaccgttct
      241 gaccgctggt ttcggtgcta tctggggtgc tatcctgcgt tacggtatca ccaactacgg
      301 taaaaaacac tggtctgaaa aattcccgta cgctaccctg ctgatcaacc tgactggcgc
      361 gttcctgctg ggttttatct tctctcgtaa attctctccg ttcatctacg ctctgatcgg
      421 taccggtgtt ctgggtggtt acaccacctt ctctaccctg aacgttgaac tgctgtctca
      481 ctggcgtgac cgtaactact ctgttttcac cctgtacgct ctgctgtctt acggtggtgg
      541 tctgatcctg gttttcctgg gttacaaagt tggtaccctg atctactaga gtcacactgg
      601 ctcaccttcg ggtgggcctt tctgcgttta ta
//