BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K2073005
1
BBa_K2073005
CrcB-La2
2016-10-11T11:00:00Z
2016-10-12T07:38:08Z
Lactobacillus johnsonii NCC 533
this is fluoride ion transporter sequence form Lactobacillus johnsonii NCC 533. we reverse translate the Cecb form protein sequence to DNA sequence.
false
false
_2541_
30741
30741
9
false
we over express the Crcb. hope the bacteria can more resistance to fluoride
false
YU-CHUN LIN
BBa_K2073013
1
BBa_K2073013
CrcB-La2 with IPTG promoter
2016-10-13T11:00:00Z
2016-10-29T04:33:09Z
E.coli
Fluoride ion can damage bacteria.
Crcb(flc) is a kind of fluoride ion transporter, which could be transported to plasma membrane and expulse fluoride ion from bacteria .
Avoid bacteria die due to fluoride.
false
false
_2541_
30752
30741
9
false
Avoid bacteria die due to fluoride.
false
WEI-CHIA HSU
component2502995
1
BBa_J04500
component2502997
1
BBa_B0012
component2502996
1
BBa_K2073005
annotation2502996
1
BBa_K2073005
range2502996
1
227
583
annotation2502995
1
BBa_J04500
range2502995
1
1
220
annotation2502997
1
BBa_B0012
range2502997
1
592
632
BBa_J04500
1
BBa_J04500
IPTG inducible promoter with RBS
2005-06-08T11:00:00Z
2015-08-31T04:08:14Z
Davidson Synth-Aces
Released HQ 2013
R0010.B0034
false
true
_16_
0
326
16
In stock
false
false
Kristen DeCelle
component1508149
1
BBa_R0010
component1508159
1
BBa_B0034
annotation1508149
1
BBa_R0010
range1508149
1
1
200
annotation1508159
1
BBa_B0034
range1508159
1
209
220
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961227
1
start
range1961227
1
173
173
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961224
1
-35
range1961224
1
137
142
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K2073013_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgatcaccgttctgaccgctggtttcggtgctatctggggtgctatcctgcgttacggtatcaccaactacggtaaaaaacactggtctgaaaaattcccgtacgctaccctgctgatcaacctgactggcgcgttcctgctgggttttatcttctctcgtaaattctctccgttcatctacgctctgatcggtaccggtgttctgggtggttacaccaccttctctaccctgaacgttgaactgctgtctcactggcgtgaccgtaactactctgttttcaccctgtacgctctgctgtcttacggtggtggtctgatcctggttttcctgggttacaaagttggtaccctgatctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J04500_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K2073005_sequence
1
atgatcaccgttctgaccgctggtttcggtgctatctggggtgctatcctgcgttacggtatcaccaactacggtaaaaaacactggtctgaaaaattcccgtacgctaccctgctgatcaacctgactggcgcgttcctgctgggttttatcttctctcgtaaattctctccgttcatctacgctctgatcggtaccggtgttctgggtggttacaccaccttctctaccctgaacgttgaactgctgtctcactggcgtgaccgtaactactctgttttcaccctgtacgctctgctgtcttacggtggtggtctgatcctggttttcctgggttacaaagttggtaccctgatc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z